结缔组织生长因子单克隆抗体超微超顺磁氧化铁粒子的制备及其生物活性的研究

单位：东南大学临床医学院心血管病研究所; 东南大学附属中大医院临床检验中心; 东南大学附属中大医院心内科; 东南大学生物科学与医学工程系;

分类号：

出版年·卷·期（页码）：2009·28·第四期（321-324）

摘要：

目的:通过化学合成方法制作结缔组织生长因子(connective tissue growth factor,CTGF)单克隆抗体超微超顺磁氧化铁(ultrasmall superparamagnetic iron oxide,USPIO)粒子分子探针,并检测其生物活性。方法:采用EDC(1-ethyl-3-[3-dimethylamin-opropyl]carbodiimide hydrochloride)化学连接剂将CTGF单克隆抗体与DMSA[meso-2,3-dimercaptosuccinic acid(HOOCCH(SH)-CH(SH)-COOH)]修饰的USPIO相结合,形成具有免疫活性的分子探针。通过间接酶联免疫吸附试验(ELISA)及Western blot-ting方法检测其生物稳定性及免疫活性。结果:随着加入不同质量的单克隆抗体,ELISA试验所得的吸光度值随抗体含量增加而增大,与阴性对照USPIO组相比较差异显著(P<0.05),分别间隔30 d及60 d后再次测量,只有200μg单抗组吸光度值降低(P<0.05)。Western blotting试验显示,结合单抗的USPIO及单纯单抗...

参考文献：

[1]	Fan WH, Pech M, Karnovsky MJ. Connective tissue growth factor (CTGF) stimulates vascular smooth muscle cell growth and migration in vitro .Eur J Cell Biol, 2000, 79(12) :915-923 .
[2]	Chen MM, Lan AA, Abraham SA, et al. CTGF expression is induced by TGFβin cardiac fibroblasts and cardiac myocytes: a potential role in heart fibrosis .J Mol Cell Cardiol, 2000, 32 :18051819 .
[3]	Finckenberg P, Lassila M, Inkinen K, et al. Cyclosprine induces myocardial connective tissue growth factor in spontaneously hypertensive rats on hig-sodium diet .Transplantation, 2001, 71(7) :951-958 .
[4]	Zhang S,Bian Z,Gu C,et al. Preparation of anti-human cardiac troponin iimmunomagnetic nanoparticles and biological activity as- says .Colloids and Surfaces B Biointerfaces, 2007, 55 (2) :143-148 .
[5]	Y. Y. Liang,L. M. Zhang. Bioconjugation of Papain on Superparamagnetic Nanoparticles Decorated with Carboxymethylated Chitosan[J] .Biomacromolecules, 2007, 8 (5) :1480-1486 .
[6]	Pan J,Yang QW. Antibody-functionalized magnetic nanoparticles for the detection of carcinoembryonic antigen using a flow-injection electrochemical device .Anal Bioanal Chem, 2007, 388 :279-286 .
[7]	Jaffer FA, Weissleder R. Seeing within: molecular imaging of the cardiovascular system .Circ Res, 2004, 94 :433-445 .
[8]	Kolodgie,FD,Petrov,A,Virmani,R,Narula,N,Verjans,JW,Weber,DK. Targeting of apoptotic macrophages and experimental atheroma with radiolabelled annexin V .Circulation, 2003, 108 :3134~3139 .
[9]	Veiseh,O,Sun,C,Gunn,J,Kohler,N,Gabikian,P,Lee,D,Bhattarai,N,Ellenbogen,R,Sze,R,Hallahan,A,Olson,J,Zhang,M. Optical and MRI multifunctional nanoprobe for targeting gliomas .Nano Lett, 2005, 5 :1003~1008 .
[10]	Weissleder,R,Mahmood,U. Molecular imaging .Radiology, 2001, 219 :316~333 .
[11]	KOOI M E,CAPPENDIJK V C,CLEUTIGENS K B,et al. Ac-cumlation of ultrasmall superparamagnetic iron oxide in humanatherosclerotic plaques can be detected byin vivomagnetic reso-nance imaging[J] .Circulation, 2003, 107 (19) :2453-2458 .

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