>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
垂盆草醇提物对人肝癌细胞HepG2的抑制作用及其机制初探
作者:黄丹丹  张伟云 
单位:南京大学医学院江苏省分子医学重点实验室
关键词:垂盆草  肝癌  抗增殖  细胞周期  抗血管生成 
分类号:
出版年·卷·期(页码):2009·28·第四期(302-306)
摘要:

目的:观察垂盆草醇提物(CCW)对人肝癌细胞HepG2生长及其肿瘤相关分子指标的影响。方法:MTT法测定CCW对HepG2细胞增殖的作用;流式细胞术观察细胞周期的改变;分别用RT-PCR及免疫细胞化学法检测CCW对VEGF、c-Myc基因及蛋白水平的影响。结果:HepG2细胞的增殖在CCW作用的第24、48和72 h均被显著地抑制;CCW(100、200μg.m l-1)可使HepG2细胞阻滞于G0/G1期;CCW可显著下调VEGF、c-Myc的mRNA及蛋白水平的表达。结论:CCW对HepG2细胞增殖具有明显的抑制作用,并且能阻止细胞进入G2/M期,这可能与c-Myc基因表达的下调密切相关;CCW能抑制HepG2细胞的VEGF分泌,初步推测CCW具有抗血管生成作用。

参考文献:

[1] Perz JF,Armstrong GL,Farrington LA,Hutin YJ, Bell BP. The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide .J Hepatol, 2006, 45 :529-538 .
 
[2] Kim MH. Flavonoids inhibit VEGF/bFGF-induced angiogenesis in vitro by inhib-iting the matrix-degrading proteases .J Cell Biochem, 2003, 89 :529-538 .
 
[3] Shukla S,MacLennan G T,Flask C A,Fu P,Mishra A,Resnick MIet al. Blockade of beta-catenin signaling by plant flavonoid apigenin suppresses prostate carcinogenesis in TRAMP mice[J] .Cancer Res, 2007, 67 :6925-6935 .
 
[4] Zhuang,WJ,Fong,CC,Cao,J,Ao,L,Leung,CH,Cheung,HY,Xiao,PG,Fong,WF,Yang,MS. Involvement of NF-κB and c-myc signaling pathways in the apoptosis of HL-60 cells induced by alkaloids of Tripterygium hypoglaucum (Levl.) Hutch .Phytochemistry, 2004, 11 :295~302 .
 
[5] Cao,QZ,Lin,ZB. Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell .Life Sci, 2006, 78 :1457~1463 .
 
[6] Cui J, Dong BW, Liang P, et al. Effect of C-Myc, Ki-67, MMP-2 and VEGF expression on prognosis of hepatocellular carcinoma patients undergoing tumor resection .World J Gastroenterol, 2004, 10 (10) :1533-1536 .
 
[7] Marina Hermes , Hartmut Osswald , Julia Mattar and Doris Kloor. Influence of an altered methylation potential on mRNA methylation and gene expression in HepG2 cells .Experimental Cell Research, 2004, 294 (2) :325-334 .
 
[8] Zhang WY,Li J,Qiu SQ,et al. Effects of the exopolysaccha-ride fraction(EPSF)froma cultivated Cordyceps sinensis onim-munocytes of H22 tumor bearing mice[J] .Fitoterapia, 2008, 79 (3) :168-173 .
 
[9] Kim WH,Park YJ,Park MR,et al. Estrogenic effects of Sedum sarmentosum Bunge in ovarie-ectomized rats .J Nutr Sci Vitaminol, 2004, 50(2) :100-105 .
 
[10] Bialecki E.S,Di Bisceglie A.M. Clinical presentation and nat-ural course of hepatocellular carcinoma .Eur J GastroenterolHepatol, 2005, (17) :485-489 .
 
[11] Adhikary S, Eilers M. Transcriptional regulation and transformation by Myc proteins [J] .Nat Rev Mol Cell Biol, 2005, 6 (8) :635-645 .
 
[12] Pelengaris S, Khan M, Evan G. c-myc: more than just a matter of life and death .Nat Rev Cancer, 2002, 2(10) :764-776 .
 
[13] Carmeliet P, Jain R K. Angiogenesis in cancer and other diseases .Nature, 2000, 407 :249-257 .
 
[14] Folkman J. What is the evidence that tumors are angiogenesis dependent .J Natl Cancer Inst, 1990, 82(1) :4-7 .
 
[15] GUO W J,DATTA S,BAND V,et al. Mel-18,a polycombgroup protein,regulates cell proliferation and senescence viatranscriptional repression of Bmi-1 and c-Myc oncoproteins[J] .Mol Biol Cell, 2007, 18 (2) :536-546 .
 
[16] OHH,KANG D G,KWONJ W,et al. Isolation of angiotens inconverting enzyme (ACE)inhibitory flavonoids from Sedumsarmentosum bunge[J] .Biol Pharm Bull, 2004, 27 :2035-2037 .
 
[17] FATTOVICHG,STROFFOLINI T,ZAGNI I,et al. Hepatocel-lular carcinoma in cirrhosis:incidence and risk factors[J] .Gastroenterol, 2004, 127 (5) :35-50 .
 
[18] COWLING V H,COLE M D. Turning the tables:Myc acti-vates Wnt in breast cancer[J] .Cell Cycle, 2007, 6 (21) :2625-2627 .
 

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 186130 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-87232481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364