血小板制剂对胃癌细胞体外培养的增殖作用-东南大学学报医学版

血小板制剂对胃癌细胞体外培养的增殖作用

单位：1. 安徽医科大学附属六安医院输血科, 安徽六安 237005;
2. 安徽医科大学附属六安医院检验科, 安徽六安 237005

分类号：R457.14;R735.2

出版年·卷·期（页码）：2025·44·第三期（363-369）

摘要：

目的: 评估血小板制剂对体外培养的胃癌细胞的增殖作用,探索可以促进胃癌细胞增殖的方法,为今后细胞治疗、扩增高质量胃癌细胞提供研究基础。方法: 采用生物信息学方法开展血小板制剂与胃癌细胞关联性研究;通过不同方法获取富血小板血浆(PRP)、正常浓度血小板血浆(PNP)、贫血小板血浆(PPP)、血小板裂解液(PL)、血小板凝胶上清液(PGS)与胎牛血清(FBS),按10%浓度分别添加至RPMI 1640培养基中并与胃癌细胞共培养48 h,于倒置相差显微镜下观察胃癌细胞形态,对传代胃癌细胞进行CCK-8实验,比较胃癌细胞增殖效率。结果: 交集基因在信号受体激活活性和受体配体活性分子功能类别中显著富集;共培养结果显示FBS组和PL组胃癌细胞生长状态良好,形态典型;而PRP组部分细胞过度分化;PNP、PPP、PGS组效果一般;CCK-8结果显示胃癌细胞在不同时间及组别测量结果差异有统计学意义;生长曲线图显示FBS组增殖速率最为迅速;进一步双因素方差分析结果表明,以FBS为参照,PL的OD值测量结果与之最为接近。结论: FBS组在6组中表现最佳,连续时间点细胞增殖活力均优于其他组,PL组显示出次优的细胞生长性能,而PRP组和PNP组效果均不显著。PL可能具有替代FBS的潜力,这为不同血小板制剂在胃癌细胞培养中的应用提供了实验依据和未来方向。

Objective: To evaluate the effect of platelet preparations on the proliferation of gastric cancer cells cultured in vitro, explore the methods that can promote the proliferation of gastric cancer cells, and provide a research basis for cell therapy and the expansion of high-quality gastric cancer cells in the future. Methods: Bioinformatics approaches were utilized to assess the association between platelet preparations and gastric cancer cell proliferation; platelet-rich plasma(PRP), platelet-normal plasma(PNP) and platelet-poor plasma(PPP), platelet lysate(PL), platelet gel supernatant(PGS) and fetal bovine serum(FBS) were obtained by different methods. Dilute RPMI 1640 medium with 10% PRP, PNP, PPP, PL, PGS, FBS, and plasma, and co-culture with gastric cancer cells for 48 hours, then cell growth was observed under an inverted microscope. The cell proliferation efficacy in passage gastric cancer cells was evaluated using CCK-8 assays. Results: Significant enrichment of intersection genes was observed in signal receptor activator activity and receptor ligand activity categories; Co-culture findings showed optimal growth and typical morphology in FBS and PL groups, with whereas PRP induced partial cells over-differentiation, and average outcomes in PNP, PPP, and PGS groups. CCK-8 assays showed statistical differences in proliferation across groups and times, with FBS having the fastest growth rate, and PL closely matching FBS in OD values upon two-way ANOVA analysis. Conclusion: FBS demonstrates optimal performance in six media, with cell proliferation consistently superior across all time points compared to other groups, followed by PL, while PRP and PNP show negligible effects. The study indicates that PL may replace FBS in gastric cancer cell culture, offering experimental support and future perspectives for platelet-based media.

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