鼻咽癌组织F-box/WD重复结构域7、细胞因子信号抑制因子1表达及其临床意义-东南大学学报医学版

鼻咽癌组织F-box/WD重复结构域7、细胞因子信号抑制因子1表达及其临床意义

单位：南方医科大学中西医结合医院放疗科, 广东广州 510000

分类号：R739.63;R446

出版年·卷·期（页码）：2024·43·第六期（821-828）

摘要：

目的:探讨鼻咽癌(NPC)组织中F-box/WD重复结构域7(FBXW7)、细胞因子信号抑制因子1(SOCS1)的表达水平及其临床意义。方法: 选取本院2018年5月至2020年5月期间住院的100例NPC患者作为研究对象,采集病理活检癌组织标本,同时收集患者临床特征资料;另选取32例慢性鼻咽炎患者作为对照组。采用免疫组织化学染色和蛋白质印迹法检测组织中FBXW7、SOCS1的表达情况,并进一步分析FBXW7、SOCS1表达水平与临床病理特征的关系;绘制乘积极限法(Kaplan-Meier)曲线分析FBXW7、SOCS1表达对患者预后生存率的影响;比例风险回归模型(Cox)回归分析影响NPC患者预后死亡的风险因素。结果: NPC组FBXW7、SOCS1阳性表达率明显低于对照组(χ²值分别为23.871、28.969,P＜0.001);FBXW7阳性表达患者3年生存率明显高于阴性表达患者(84.38% vs 54.41%,P=0.002),SOCS1阳性表达患者3年生存率明显高于阴性表达患者(93.94% vs 49.25%,P＜0.001);FBXW7和SOCS1阴性表达是NPC患者死亡的危险因素(P＜0.05)。结论: 在NPC患者组织中FBXW7和SOCS1阳性表达率较低,二者表达与NPC患者预后密切相关。

Objective: To investigate the expression levels and clinical significance of F-box and WD repeat domain containing 7(FBXW7) and suppressor of cytokine signaling 1(SOCS1) in nasopharyngeal carcinoma(NPC) tissue. Methods: One hundred NPC patients who were hospitalized in our hospital during the period from May 2018 to May 2020 were selected as the study subjects, and pathological biopsy cancer tissue specimens were collected, as well as data on the clinical characteristics of the patients; another 32 patients with chronic nasopharyngitis were selected as the control group. Immunohistochemical staining and protein immunoblotting were applied to detect the expression of FBXW7 and SOCS1 in tissues, the relationship between the expression levels of FBXW7, SOCS1 and clinical pathological features was further analyzed; Kaplan-Meier curves were plotted to analyse the effect of FBXW7 and SOCS1 expression on the prognostic survival of patients; proportional hazard regression model(Cox) regression analysis of risk factors affecting prognostic mortality in NPC patients was done. Results: The positive expression rates of FBXW7 and SOCS1 in the NPC group were significantly lower than those in the control group(χ²:23.871, 28.969, P<0.001); the 3-year survival rate was significantly higher in patients with positive expression of FBXW7 than that in patients with negative expression(84.38% vs 54.41%, P=0.002), and the 3-year survival rate was significantly higher in patients with positive expression of SOCS1 than that in patients with negative expression(93.94% vs 49.25%, P<0.001); negative expression of FBXW7 and SOCS1 was a risk factor for death in NPC patients(P<0.05). Conclusion: In the tissues of NPC patients, the positive expression rates of FBXW7 and SOCS1 are relatively low, and the expression of both is closely associated with the prognosis of NPC patients.

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