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多发性骨髓瘤患者血清lncRNA PITPNA-AS1、lncRNA NORAD水平及其与患者预后的关系
作者：李红伟  司松环  杨靖  刘艳杰 
单位：郑州大学第一附属医院 血液科, 河南 郑州 450000
关键词：多发性骨髓瘤 长链非编码RNA PITPNA反义RNA1 长链非编码RNA NORAD 预后 
分类号：R733.3
出版年·卷·期（页码）：2024·43·第三期（450-455）
摘要：

目的: 分析多发性骨髓瘤(MM)患者血清长链非编码RNA(lncRNA) PITPNA反义RNA1(PITPNA-AS1)、lncRNA NORAD水平及其与患者预后的关系。方法:分别选择本院收治的96例MM患者和96例来本院健康查体的志愿者作为试验组和对照组,时间在2018年1月至2020年12月期间;采用实时荧光定量PCR(RT-qPCR)法检测两组血清中lncRNA PITPNA-AS1、lncRNA NORAD的相对表达量;采用Pearson相关分析分析血清lncRNA PITPNA-AS1与lncRNA NORAD水平的相关性;采用Kaplan-Meier生存分析分析血清lncRNA PITPNA-AS1、lncRNA NORAD水平与MM预后的关系;采用多元Cox回归分析MM患者预后的影响因素。结果:试验组血清lncRNA PITPNA-AS1、lncRNA NORAD水平显著高于对照组(P＜0.05)。Ⅲ期MM患者血清lncRNA PITPNA-AS1、lncRNA NORAD水平显著高于Ⅰ期和Ⅱ期,Ⅱ期显著高于Ⅰ期(P＜0.05)。MM患者血清lncRNA PITPNA-AS1与lncRNA NORAD水平呈正相关(r=0.636,P＜0.05)。lncRNA PITPNA-AS1和lncRNA NORAD高表达患者3年总生存率均低于低表达患者(χ2值分别为8.065、11.937,P值分别为0.005、0.001)。ISS分期较高、lncRNA PITPNA-AS1高水平、lncRNA NORAD高水平是MM患者预后的危险因素(P＜0.05)。结论:MM患者血清lncRNA PITPNA-AS1、lncRNA NORAD水平异常升高,且与患者预后关系密切。

Objective: To analyze the serum levels of long non-coding RNA(lncRNA) PITPNA antisense RNA1(PITPNA-AS1), lncRNA NORAD in patients with multiple myeloma(MM), and their relationship with prognosis. Methods: 96 patients with MM admitted to our hospital and 96 volunteers who came to our hospital for health checkups were selected as the experimental and control groups during January 2018 to December 2020, respectively; real-time fluorescence quantitative PCR(RT-qPCR) method was applied to detect the relative expression levels of serum lncRNA PITPNA-AS1 and lncRNA NORAD in two groups; Pearson correlation was applied to analyze the correlation between serum lncRNA PITPNA-AS1 and lncRNA NORAD levels; Kaplan-Meier survival was utilized to analyze the association between serum lncRNA PITPNA-AS1, lncRNA NORAD levels and MM prognosis; multiple Cox regression was utilized to analyze the factors influencing the prognosis of MM patients. Results: The serum levels of lncRNA PITPNA-AS1 and lncRNA NORAD in the experimental group were greatly higher than those in the control group(P＜0.05). The serum levels of lncRNA PITPNA-AS1 and lncRNA NORAD in patients with stage Ⅲ MM were greatly higher than those in stage Ⅰand Ⅱ, while the serum levels of lncRNA PITPNA-AS1 and lncRNA NORAD in patients with stage Ⅱ MM were greatly higher than those in stage Ⅰ(P＜0.05). There was a positive correlation between serum lncRNA PITPNA-AS1 and lncRNA NORAD levels in patients with MM(r=0.636, P＜0.05). Patients with high expression of both lncRNA PITPNA-AS1 and lncRNA NORAD had lower 3-year overall survival than patients with low expression(χ2:8.065, 11.937; P:0.005, 0.001). Higher ISS staging, high levels of lncRNA PITPNA-AS1, and high levels of lncRNA NORAD were prognostic risk factors for MM patients(P＜0.05). Conclusion: The serum levels of lncRNA PITPNA-AS1 and lncRNA NORAD are abnormally elevated in patients with MM, and are closely related to prognosis.
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