超声联合血清miR-106a-5p、miR-20b-5p对糖尿病足下肢血管病变的诊断价值-东南大学学报医学版

超声联合血清miR-106a-5p、miR-20b-5p对糖尿病足下肢血管病变的诊断价值

单位：监利市人民医院超声影像科, 湖北监利 433300

关键词：糖尿病足下肢血管病变超声微小RNA-106a-5p 微小RNA-20b-5p 诊断价值

分类号：R587.2

出版年·卷·期（页码）：2024·43·第三期（439-444）

摘要：

目的:探究超声联合血清miR-106a-5p、miR-20b-5p对糖尿病足(DF)下肢血管病变(LEVD)的诊断价值。方法:选取2021年3月至2023年6月在我院就诊的90例DF患者为研究对象,根据是否合并LEVD分为LEVD组42例和非LEVD组48例。采用实时荧光定量PCR检测DF患者血清miR-106a-5p、miR-20b-5p表达水平,并对所有患者进行下肢血管超声检查。采用受试者工作特征(ROC)曲线分析血清miR-106a-5p、miR-20b-5p对DF患者合并LEVD的诊断价值;采用一致性Kappa检验分析超声单独及联合血清miR-106a-5p、miR-20b-5p与金标准的一致性。结果:超声检查结果与金标准诊断结果一致性较高,Kappa值为0.642(P＜0.05)。LEVD组血清miR-106a-5p、miR-20b-5p水平显著高于非LEVD组(P＜0.05)。血清miR-106a-5p、miR-20b-5p诊断DF患者发生LEVD的曲线下面积(AUC)分别为0.803、0.757。血清miR-106a-5p、miR-20b-5p检查结果与金标准诊断结果一致性均为中度,Kappa值分别为0.503、0.492(P＜0.05)。超声联合血清miR-106a-5p、miR-20b-5p水平检查结果与金标准诊断结果一致性极高,Kappa值为0.866(P＜0.05)。超声联合血清miR-106a-5p、miR-20b-5p诊断DF患者LEVD的准确度、阴性预测值显著高于三者单独诊断(P＜0.05)。结论:超声联合血清miR-106a-5p、miR-20b-5p诊断DF患者LEVD的价值较高,值得推广。

Objective: To explore the diagnostic value of ultrasound combined with serum miR-106a-5p, miR-20b-5p for lower extremity vascular disease(LEVD) of diabetic foot(DF). Methods: Ninety patients with DF who visited our hospital from March 2021 to June 2023 were selected as the study subjects. They were grouped into a LEVD group of 42 cases and a non LEVD group of 48 cases based on whether they were complicated with LEVD. Real-time fluorescence quantitative PCR was applied to detect the expression levels of miR-106a-5p and miR-20b-5p in serum of DF patients, and lower extremity vascular ultrasound examination was performed on all patients. The diagnostic value of serum miR-106a-5p and miR-20b-5p in DF patients with LEVD was analyzed using the receiver operating characteristic(ROC) curve; the consistency Kappa test was applied to analyze the consistency between ultrasound alone and in combination of serum miR-106a-5p, miR-20b-5p, with the gold standard. Results: The consistency between the ultrasound examination results and the gold standard diagnostic results was high, with a Kappa value of 0.642(P＜0.05). The serum levels of miR-106a-5p and miR-20b-5p in the LEVD group were obviously higher than those in the non LEVD group(P＜0.05). The area under the curve(AUC) of serum miR-106a-5p and miR-20b-5p for diagnosing LEVD in DF patients was 0.803 and 0.757, respectively. The consistency between the serum miR-106a-5p, miR-20b-5p test results and the gold standard diagnostic results was moderate, with Kappa values of 0.503 and 0.492, respectively(P＜0.05). The results of ultrasound combined with serum miR-106a-5p, miR-20b-5p levels were highly consistent with the gold standard diagnostic results, with a Kappa value of 0.866(P＜0.05). The accuracy, and negative predictive value of ultrasound combined with serum miR-106a-5p and miR-20b-5p in diagnosing LEVD in DF patients were obviously higher than those of the three alone(P＜0.05). Conclusion: Ultrasound combined with serum miR-106a-5p and miR-20b-5p have high diagnostic value for LEVD in DF patients and are worth promoting.

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