Objective: To investigate the correlation between peripheral blood microrna(miR)-106a and miR-20a expression and chest X-ray(CXR) manifestations and inflammation in children with Mycoplasma pneumoniae pneumonia(MPP). Methods: 393 children with MPP admitted to our hospital from January 2020 to December 2021 were selected as research objects. White blood cell count,red blood cell count,platelet count and neutral lobed nucleus leukocyte were measured by automatic blood cell analyzer,and C-reactive protein(CRP) was detected by immunoturbidimetry. The expression of miR-106a and miR-20a in peripheral blood was detected by real-time quantitative polymerase chain reaction. All patients underwent CXR examination after enrollment. Results: The expression levels of miR-106a and miR-20a in peripheral blood of MPP patients were 1.00(0.61,2.11) and 1.06(0.73,1.67),respectively. After grouping based on median values,compared with miR-106a and miR-20a low expression groups,the level of CRP and the proportion of lung consolidation patients in the high expression group were significantly higher(P<0.05). Moreover,the proportion of children with pleural effusion in the high expression group of miR-20a was significantly higher than that in the low expression group(P<0.05). There was a significant positive correlation between peripheral blood miR-106a and miR-20a in MPP patients and CRP(r=0.212,0.230,P<0.001). The expression levels of miR-106a and miR-20a in peripheral blood of the lung consolidation group were significantly higher than those of the non lung consolidation group(P<0.05). According to ROC curve analysis,the area under the curve(AUC) of peripheral blood miR-106a and miR-20a for predicting MPP lung consolidation were 0.811(95%CI:0.767-0.855) and 0.807(95%CI:0.762-0.852),with a sensitivity of 75.3% and 77.4%,a specificity of 76.5% and 76.4%,and corresponding cutoff values of 1.20 and 1.18,respectively. Logistic regression analysis showed that high expression of miR-106a and miR-20a in peripheral blood was an independent influencing factor for MPP pulmonary consolidation(P<0.05).Conclusion: The overexpression of miR-106a and miR-20a in peripheral blood of children with MPP is associated with severe CXR manifestations and increased inflammation,which may be used as markers to indicate the severity of MPP and the progression of inflammation. |
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