盆腔炎性疾病患者血清miR-140-5p、TLR4的表达水平及其临床意义-东南大学学报医学版

盆腔炎性疾病患者血清miR-140-5p、TLR4的表达水平及其临床意义

单位：成都市中西医结合医院·成都市第一人民医院妇产科, 四川成都 610095

分类号：R711.33

出版年·卷·期（页码）：2023·42·第六期（909-914）

摘要：

目的:探讨盆腔炎性疾病(PID)患者血清中miR-140-5p、Toll样受体4(TLR4)的表达水平及其临床意义,旨在为诊断和治疗PID患者提供相关依据。方法:选取2020年10月至2021年10月本院收治的132例PID患者为PID组,100例体检健康人群作为对照组;采用实时荧光定量PCR(RT-qPCR)技术检测miR-140-5p、TLR4表达水平;采用酶联免疫吸附法(ELISA)检测TNF-α、IL-6、IL-4和IL-1β的含量;Pearson法分析miR-140-5p、TLR4的相关性以及二者与TNF-α、IL-6、IL-4、IL-1β的相关性;绘制ROC曲线分析miR-140-5p、TLR4对PID的诊断价值。结果:PID组血清miR-140-5p表达水平显著低于对照组,TLR4的表达水平显著高于对照组(P＜0.05),二者表达水平具有负相关关系(P＜0.05);随着PID严重程度增加miR-140-5p的表达水平降低,TLR4的表达水平升高(P＜0.05);PID组患者血清TNF-α、IL-6、IL-1β水平较对照组显著升高,IL-4水平显著降低(P＜0.05);miR-140-5p与TNF-α、IL-6、IL-1β水平均呈负相关,与IL-4水平呈正相关(P＜0.05);TLR4与TNF-α、IL-6、IL-1β水平均呈正相关,与IL-4水平呈负相关(P＜0.05);二者联合诊断的AUC高于miR-140-5p、TLR4单独诊断的AUC(Z=2.214,P＜0.001;Z=2.012,P=0.031)。结论:PID患者血清中miR-140-5p表达水平显著降低,TLR4表达水平显著升高,二者联合对PID具有一定的诊断价值。

Objective: To investigate the expression level of miR-140-5p and Toll-like receptor 4(TLR4) in serum of patients with pelvic inflammatory disease(PID) and their clinical significance, in order to provide relevant basis for diagnosis and treatment of patients with PID. Methods: From October 2020 to October 2021, 132 PID patients admitted to our hospital were included in the PID group, and 100 healthy people with physical examination were included in the control group; real-time quantitative PCR(RT-qPCR) technology was applied to detect the expression levels of miR-140-5p and TLR4; enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of TNF-α, IL-6, IL-4 and IL-1β; Pearson method was applied to analyze the correlation between miR-140-5p and TLR4 and their correlation with TNF-α, IL-6, IL-4 and IL-1β; ROC curve was drawn to analyze the diagnostic value of miR-140-5p and TLR4 for PID. Results: The expression level of serum miR-140-5p in the PID group was greatly lower than that in the control group, and the expression level of TLR4 was greatly higher than that in the control group(P<0.05), and the two expression levels were negatively correlated(P<0.05); with the increase of PID severity, the expression level of miR-140-5p decreased, and the expression level of TLR4 increased(P<0.05); the serum levels of TNF-α, IL-6 and IL-1β in the PID group were greatly higher than those in the control group, and the level of IL-4 was greatly lower(P<0.05); miR-140-5p was negatively correlated with the levels of TNF-α, IL-6 and IL-1β, and positively correlated with the level of IL-4(P<0.05); TLR4 was positively correlated with the levels of TNF-α, IL-6, IL-1β, and negatively correlated with the level of IL-4(P<0.05); the AUC of the combined diagnosis was higher than that of miR-140-5p and TLR4 alone(Z=2.214,P<0.001;Z=2.012,P=0.031). Conclusion: The expression level of miR-140-5p in serum of PID patients was greatly decreased, and the expression level of TLR4 was greatly increased. The combination of the two has certain diagnostic value for PID.

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