非小细胞肺癌组织中LINC00707、miR-382-5p表达及其与患者预后的关系-东南大学学报医学版

非小细胞肺癌组织中LINC00707、miR-382-5p表达及其与患者预后的关系

单位：武汉市第三医院/武汉大学附属同仁医院呼吸与危重症医学科, 湖北武汉 430074

关键词：非小细胞肺癌非编码RNA 00707 微小RNA-382-5p 临床病理特征预后

分类号：R734

出版年·卷·期（页码）：2023·42·第五期（724-729）

摘要：

目的:探讨非小细胞肺癌(NSCLC)组织中非编码RNA 00707(LINC00707)、微小RNA-382-5p(miR-382-5p)表达及其与患者预后的关系。方法:选取本院2018年2月至2020年2月收治的127例NSCLC患者术中留取的NSCLC组织和癌旁组织标本。采用Pearson法分析NSCLC组织LINC00707与miR-382-5p表达的相关性;采用Kaplan-Meier法分析NSCLC组织LINC00707、miR-382-5p表达与患者预后关系;采用多因素Cox回归分析NSCLC患者预后的影响因素。结果:与癌旁组织相比,NSCLC组织中LINC00707表达水平较高,miR-382-5p表达水平较低(P<0.05);NSCLC组织中LINC00707与miR-382-5p表达水平呈负相关(r=-0.692,P<0.05);不同年龄、性别、肿瘤直径、浸润深度、组织学分类的NSCLC患者LINC00707、miR-382-5p表达水平比较,差异均无统计学意义(P>0.05),而TNM分期为Ⅲ+Ⅳ期、淋巴结发生转移、分化程度为低分化的患者NSCLC组织中LINC00707呈现高表达水平,miR-382-5p则呈现出低表达水平(P<0.05);LINC00707低表达患者3年生存率(27/63,42.86%)高于LINC00707高表达患者(18/64,28.13%)(χ²=5.123,P=0.024),miR-382-5p高表达患者3年生存率(28/64,43.75%)高于miR-382-5p低表达患者(17/63,26.98%)(χ²=4.089,P=0.043);TNM分期、淋巴结转移、分化程度、LINC00707、miR-382-5p是NSCLC患者死亡的影响因素(P<0.05)。结论:NSCLC组织中LINC00707表达较高,miR-382-5p表达较低,二者与NSCLC患者TNM分期、淋巴结转移、分化程度等临床病理特征密切相关,同时也是影响NSCLC患者预后的敏感指标。

Objective: To investigate the expression of LINC00707 and miR-382-5p in non-small cell lung cancer(NSCLC) tissue and their relationship with prognosis. Methods: A total of 127 NSCLC patients admitted to our hospital from February 2018 to February 2020 were selected, NSCLC tissue and adjacent tissue samples were collected during surgery. Pearson method was applied to analyze the correlation between LINC00707 and miR-382-5p expression in NSCLC tissue; Kaplan-Meier method was applied to analyze the relationship between the expression of LINC00707 and miR-382-5p in NSCLC tissue prognosis; and multivariate Cox regression analysis was applied to analyze the prognostic factors affecting NSCLC patients. Results: Compared with adjacent tissue, the expression level of LINC00707 in NSCLC tissue was higher, while the expression level of miR-382-5p was lower(P<0.05); the expression level of LINC00707 in NSCLC tissue was negatively correlated with miR-382-5p(r=-0.692, P<0.05). There was no statistically significant difference in the expression levels of LINC00707 and miR-382-5p among NSCLC patients of different ages, genders, tumor diameter, invasion depth, and histological classification(P>0.05). However, LINC00707 showed high expression level in patients with TNM stage Ⅲ+Ⅳ, lymph node metastasis, and low differentiation, while miR-382-5p showed low expression level(P<0.05).The 3-year survival rate of patients with low expression LINC00707(27/63, 42.86%) was higher than that of patients with high expression LINC00707(18/64, 28.13%)(χ²=5.123, P=0.024), the 3-year survival rate of patients with high expression of miR-382-5p(28/64, 43.75%) was higher than that of patients with low expression of miR-382-5p(17/63, 26.98%)(χ²=4.089, P=0.043). TNM staging, lymph node metastasis, degree of differentiation, LINC00707, and miR-382-5p were influencing factors for death in NSCLC patients(P<0.05). Conclusion: The expression of LINC00707 is higher and miR-382-5p is lower in NSCLC tissue, the two are closely related to clinical pathological features such as TNM staging, lymph node metastasis, and degree of differentiation in NSCLC patients, and are also sensitive indicators that affect the prognosis of NSCLC patients.

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