目的: 以微量中和试验为金标准,评价胶体金法和酶联免疫吸附法这两种高通量检测方法的检测能力。方法: 以江苏省南京市某社区招募的250名健康成年人为研究对象,采集血样开展中和抗体水平分析,用微量中和试验、胶体金法和酶联免疫吸附法分别对血样进行测试,评价这两种高通量中和抗体检测方法的灵敏度、特异度及与金标准的相关系数等。结果: 当金标准的判定界值取为1∶4时(<4为阴性),胶体金法的灵敏度为97.50%,特异度为98.82%,正确指数为0.963,与金标准之间的Kappa值为0.963 2;酶联免疫吸附法的灵敏度为95.00%,特异度为100.00%,正确指数为0.950,Kappa值为0.962 7。这两种方法相对于微量中和试验的相关系数为0.963。结论: 建立的新型冠状病毒中和抗体高通量检测方法具有科学性和可行性。 |
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