目的：探讨贫血小板血浆（PPP）、富血小板血浆（PRP）及血小板凝胶上清液（PGS）对大肠埃希菌的体外抑菌作用及其与抗菌肽血小板因子4（PF4）含量的关系。方法：从本市中心血站获取健康献血者的单采血小板作为PRP来源，采用平板培养细菌法观察PPP、PRP及PGS与大肠埃希菌共培养不同时间细菌生长情况，ELISA检测PPP、PRP及PGS中PF4的含量。结果：（1） PPP、PRP及PGS均可以抑制大肠埃希菌的生长。PPP、PRP在18 h内具有很强的抑菌作用，随后抑菌作用逐渐减弱，24 h后逐渐失去抑菌作用；PGS在9 h内具有抑菌作用，3 h时抑菌作用最佳，随后抑菌作用逐渐减弱，12 h时无抑菌作用。（2）凝胶缓释液也具有抑菌作用，抑菌时效同PGS。（3） PPP、PRP和PGS中PF4质量浓度分别为（300.65±75.46）、（23 206.74±9 753.90）、（5 659.23±2 847.99） ng·ml^-1，差异有统计学意义。（4） PRP 24 h抑菌作用强弱与PF4含量无关，差异无统计学意义（P>0.05）。结论：PPP、PRP及PGS对大肠埃希菌均有抑菌作用，但有时效性，PPP与PRP抑菌效果一致，均优于PGS。同体积PRP中PF4含量高于PGS，PPP中PF4含量最低，但PRP在24 h的抑菌作用强弱与PF4含量无关。

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