复发性流产患者绒毛组织HIF-1α、VHL蛋白的表达及其临床意义-东南大学学报医学版

复发性流产患者绒毛组织HIF-1α、VHL蛋白的表达及其临床意义

单位：郑州大学附属郑州中心医院妇产科, 河南郑州 450000

分类号：R714.21

出版年·卷·期（页码）：2023·42·第一期（98-103）

摘要：

目的：检测复发性流产患者绒毛组织缺氧诱导因子-1α（HIF-1α）、希佩尔林道抑癌基因（VHL）蛋白表达水平并分析其临床意义。方法：选取2019年5月至2021年11月本院收治的83例复发性流产患者作为观察组，另选取80例行人工流产的正常早孕者设为对照组。收集两组绒毛组织，采用免疫组化法检测绒毛组织HIF-1α、VHL蛋白表达水平，采用Spearman法分析复发性流产患者HIF-1α与VHL蛋白表达的关系，并采用Logistic回归分析复发性流产的影响因素。结果：观察组绒毛组织HIF-1α蛋白阳性表达率高于对照组（P<0.05），VHL蛋白阳性表达率低于对照组（P<0.05）；观察组HIF-1α蛋白表达与VHL蛋白表达呈负相关（r=-0.504，P<0.05）；观察组孕妇被动吸烟、人工流产≥3次、作息不规律占比均高于对照组（P<0.05）；经Logistic回归分析可知，人工流产≥3次、作息不规律及HIF-1α蛋白阳性表达率升高均是发生复发性流产的危险因素（P<0.05），VHL蛋白阳性表达是其保护因素（P<0.05）。结论：复发性流产患者绒毛组织中HIF-1α蛋白表达水平异常升高，VHL蛋白表达水平异常降低，且两者呈负相关，均可能与复发性流产的发生有关。

Objective: To detect the expressions of hypoxia inducible factor-1 α(HIF-1α) and Von Hippel Lindau tumor suppressor gene(VHL) protein in villi of patients with recurrent abortion and analyze their clinical significance. Methods: 83 patients with recurrent abortion treated in our hospital from May 2019 to November 2021 were selected into observation group, and 80 normal early pregnant patients with induced abortion were selected into control group. The villi tissues of the two groups were collected, and the expressions of HIF-1α and VHL protein in villi were detected by immunohistochemical method. The relationship between HIF-1α protein expression and VHL protein expression in patients with recurrent abortion was analyzed by Spearman method, and the influencing factors of recurrent abortion were analyzed by Logistic regression. Results: The positive expression rate of HIF-1α protein in villi of the observation group was higher than that of the control group(P<0.05), and the positive expression rate of VHL protein was lower than that of the control group(P<0.05). The expression of HIF-1α protein in villi of patients with recurrent abortion was negatively correlated with that of VHL protein(r=-0.504, P<0.05). The proportion of passive smoking of pregnant women, induced abortion ≥ 3 times, irregular work in the observation group were higher than those in the control group(P<0.05). Logistic regression analysis showed that induced abortion ≥ 3 times, irregular schedule and high positive expression rate of HIF-1α protein were the risk factors for recurrent abortion(P<0.05), and the positive expression of VHL protein was the protective factor(P<0.05). Conclusion: The expression of HIF-1α protein in villi of patients with recurrent abortion is abnormally increased, and the expression of VHL is abnormally decreased. The expression of the two proteins is correlated, which may be related to the occurrence of recurrent abortion.

参考文献：

[1] AKBARI S, SHAHSAVAR F, KARAMI R, et al.Recurrent spontaneous abortion(RSA) and maternal KIR genes:a comprehensive meta-analysis[J].JBRA Assist Reprod, 2020, 24(2):197-213.
[2] 袁惠芝, 郑玉华, 冯敏清, 等.复发性流产与调节性T细胞及IL-10的相关性分析[J].现代医学, 2019, 47(12):1487-1490.
[3] DEVALL A J, COOMARASAMY A.Sporadic pregnancy loss and recurrent miscarriage[J].Best Pract Res Clin Obstet Gynaecol, 2020, 69(2):30-39.
[4] 徐英姬, 周红燕.补肾化瘀固胎方辅助治疗复发性流产血栓前状态的临床观察[J].中国中医药科技, 2021, 28(4):669-671.
[5] 李光苗, 陈飞, 王燕.丹参川芎嗪与硫酸镁对妊娠期高血压疾病大鼠的治疗效果及对胎盘组织缺氧诱导因子-1α、可溶性血管内皮生长因子受体-1表达的影响[J].解剖学杂志, 2021, 44(5):389-393.
[6] HOEFFLIN R, HARLANDER S, SCHÄFER S, et al.HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice[J].Nat Commun, 2020, 11(1):4111.
[7] LIU J, XU B, ZHENG C, et al.An end-to-end deep learning histochemical scoring system for breast cancer TMA[J].IEEE Trans Med Imag, 2019, 38(2):617-628.
[8] 陈水莲, 梁志江, 黄冠豪, 等.广东省育龄妇女自然流产发生情况及其影响因素分析[J].中国生育健康杂志, 2020, 31(2):119-122, 126.
[9] 蔡丹, 唐新桥, 刘霞, 等.不同流产次数的复发性流产患者血栓弹力图比较研究[J].中国计划生育和妇产科, 2020, 12(1):34-37, 47.
[10] 王悦菲, 虞丰, 秦岭, 等.稽留流产患者VEGF、Hcy及HIF-1α水平及危险因素分析[J].中国计划生育学杂志, 2020, 28(7):1078-1081.
[11] 余艳茹, 许雅娟, 翟闪闪, 等.胎盘绒毛CD81、HIF-1α、VEGF在早孕期复发性流产中的表达[J].安徽医科大学学报, 2019, 54(4):611-616.
[12] CHEN X, JIANG L, WANG C C, et al.Hypoxia inducible factor and microvessels in peri-implantation endometrium of women with recurrent miscarriage[J].Fertil Steril, 2016, 105(6):1496-1502.
[13] 苏倩, 凌琳, 虞红珍, 等.复发性流产患者微小RNA155与子宫内膜HIF-1αVEGF微血管密度的关系[J].安徽医学, 2022, 43(3):314-317.
[14] VAN ROOIJEN E, VAN DE HOEK G, LOGISTER I, et al.The Von Hippel-Lindau gene is required to maintain renal proximal tubule and glomerulus integrity in zebrafish larvae[J].Nephron, 2018, 138(4):310-323.
[15] CARGILL K, HEMKER S L, CLUGSTON A, et al.Von Hippel-Lindau acts as a metabolic switch controlling nephron progenitor differentiation[J].J Am Soc Nephrol, 2019, 30(7):1192-1205.
[16] 徐峰, 王莹, 鲁晓燕, 等.HIF-1α、TLR4与稽留流产妊娠滋养细胞凋亡的关系研究[J].中国计划生育和妇产科, 2020, 12(9):73-77, 前插1.
[17] MCCRACKEN S A, SEEHO S K M, CARRODUS T, et al.Dysregulation of oxygen sensing/response pathways in pregnancies complicated by idiopathic intrauterine growth restriction and early-onset preeclampsia[J].Int J Mol Sci, 2022, 23(5):2772-2774.
[18] 郝乐乐, 李伟莉, 吴花, 等.补肾安胎冲剂调控VHL/HIF-1α信号通路改善复发性流产小鼠母胎界面血管生成的实验研究[J].安徽中医药大学学报, 2020, 39(1):65-70.
[19] 钟丽荣.不明原因复发性流产的影响因素分析[J].现代诊断与治疗, 2020, 31(21):3431-3433.
[20] 唐固平, 李昭会, 陈丽融, 等.四川省达州市育龄女性复发性流产影响因素分析[J].华南预防医学, 2020, 46(5):481-485.

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