Objective: To investigate the expression of casein kinase 1 alpha1(CSNK1α1) in intrahepatic cholangiocarcinoma(ICC) tissues and its effect on tumor cell metastasis and angiogenesis. Methods: Fifty ICC tissue samples and adjacent tissue samples from Affiliated Tumor Hospital of Xinjiang Medical University were collected, and the expression level of CSNK1α1 was detected by real-time quantitative PCR(qRT-PCR) and immunohistochemical staining. The ICC cell line HUCCT-1 was cultured and randomly divided into control group, si-NC group and si-CSNK1α1 group. The short hairpin RNA sequence targeting CSNK1α1 gene was transfected into HUCCT-1 cells by liposome-mediated method, the expression changes of CSNK1α1 mRNA and protein in cells were measured by qRT-PCR and Western blotting to identify the transfection effect. Cell scratch assay and Transwell assay were used to detect changes in cell migration and invasion abilities, cell immunofluorescence staining was used to observe the expression intensity of intracellular epithelial-mesenchymal transition markers E-cadherin and Vimentin, angiogenesis assays was used to detect tumor angiogenesis, the protein expression changes of vascular endothelial growth factor(VEGF), matrix metallopeptidase(MMP) 2(MMP-2) and MMP-9 in cells were detected by Western blotting. Results: The relative expression of CSNK1α1 mRNA in ICC tissue was significantly higher than that in adjacent tissue, and the positive rate of CSNK1α1 in ICC tissue was also significantly higher than that in adjacent tissue(P<0.01). Compared with the control group and si-NC group, the relative expression of CSNK1α1 mRNA and protein in HUCCT-1 cells of si-CSNK1α1 group were down-regulated, the wound healing rate of cells decreased, the number of cell migration and invasion decreased, the fluorescence density value of cadherin decreased while the fluorescence density value of Vimentin increased, and the number of cell tube-like formation decreased, at the same time, the relative proteins expressions of VEGF, MMP-2 and MMP-9 in cells were significantly down-regulated, the differences were statistically significant(P<0.05). Conclusion: CSNK1α1 is highly expressed in ICC tissues, targeted down-regulation of CSNK1α1 expression in ICC cells can inhibit cell migration, invasion and epithelial-mesenchymal transition, and reduce angiogenesis, thereby inhibiting tumor progression. |
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