血清miR-30c-2-3p与卵巢癌组织内质网应激相关蛋白表达及患者预后的相关性-东南大学学报医学版

血清miR-30c-2-3p与卵巢癌组织内质网应激相关蛋白表达及患者预后的相关性

单位：沧州市中心医院妇科, 河北沧州 061000

分类号：R737.31

出版年·卷·期（页码）：2022·41·第五期（681-687）

摘要：

目的：分析血清miR-30c-2-3p与卵巢癌（OC）组织内质网应激（ERS）相关蛋白表达、患者临床特征及预后的关系。方法：招募2018年1月至2020年12月期间70例于我院接受治疗的OC患者（OC组），并纳入同期100例健康志愿者作为对照组。采用蛋白质印迹法分析OC组织C/EBP同源蛋白（CHOP）、葡萄糖调节蛋白78（BIP）的表达情况。使用定量实时聚合酶链反应检测血清miR-30c-2-3p表达。随访记录患者的无病生存期（DFS）。结果：OC组患者基线血清miR-30c-2-3p表达显著低于对照组受试者（0.77±0.25 vs. 1.15±0.39，t=7.869，P<0.001）。经受试者工作特征曲线分析，基线血清miR-30c-2-3p表达诊断OC的曲线下面积为0.785（95%CI 0.718~0.852）。基线血清miR-30c-2-3p表达≤0.703的患者FIGO分期Ⅲ~Ⅳ期概率更大，肿瘤直径也更大（P<0.05）。基线血清miR-30c-2-3p表达水平与OC组织CHOP表达呈正相关（r=0.483，P<0.001），与BIP表达呈显著负相关（r=-0.240，P=0.045）。经多元线性回归分析，OC组织中CHOP （t=4.080，P<0.001）、BIP （t=-2.048，P=0.045）都是影响基线血清miR-30c-2-3p表达水平的独立因子。绘制Kaplan-Meier生存曲线，基线血清miR-30c-2-3p低表达OC患者中位DFS显著短于高表达者（χ²=7.515，P=0.006）。经多因素COX回归分析显示，基线血清miR-30c-2-3p低表达为OC患者术后复发的独立临床因素（OR=0.399，95%CI 0.201~0.791，P=0.009）。结论：血清miR-30c-2-3p在OC患者中多呈低表达，并可能通过调节ERS影响患者预后。因此，血清miR-30c-2-3p表达对OC患者术后复发具有预测价值。

Objective: To analyze the relationship between serum miR-30c-2-3p and tissue expressions of endoplasmic reticulum stress(ERS) related protein, clinical features and prognosis of ovarian cancer(OC). Methods: 70 OC patients(OC group) who underwent treatment in our hospital from January 2018 to December 2020 were recruited, and a total 100 healthy volunteers during the same period were included as the control group. Western blot was used to analyze the expressions of C/EBP homologous protein(CHOP) and glucose regulated protein 78(BIP) in OC tissues. Serum miR-30c-2-3p expression was detected by quantitative real-time polymerase chain reaction. Patients' disease-free survival(DFS) was recorded during follow-up. Results: The baseline serum miR-30c-2-3p expression of OC group was significantly lower than that of the control group(0.77±0.25 vs. 1.15±0.39, t=7.869, P<0.001). According to the analysis of the receiver operating characteristic curve, the area under the curve of baseline serum miR-30C-2-3p expression for OC diagnosis was 0.785(95%CI 0.718-0.852). Patients with baseline serum miR-30c-2-3p ≤ 0.703 had higher probability of FIGO Ⅲ to Ⅳ staging and larger tumor diameter(P<0.05). The expression level of miR-30c-2-3p in baseline serum was positively correlated with the expression of CHOP in OC tissue(r=0.483, P<0.001), and significantly negatively correlated with the expression of BIP(r=-0.240, P=0.045). By multiple linear regression analysis, CHOP(t=4.080, P<0.001) and BIP(t=-2.048, P=0.045) in OC tissues were independent factors that affected the expression level of miR-30c-2-3p in baseline serum. Kaplan-Meier survival curve was plotted, and the median DFS of OC patients with low baseline serum miR-30c-2-3p expression was significantly shorter than that of OC patients with high expressionχ²=7.515, P=0.006). Multivariate COX regression analysis showed that the low baseline serum miR-30C-2-3p expression was an independent clinical factor for postoperative recurrence in OC patients(OR=0.399, 95%CI 0.201-0.791, P=0.009). Conclusion: Serum miR-30c-2-3p is low expressed in OC patients and influences the prognosis of patients by regulating ERS. Therefore, serum miR-30c-2-3p expression has predictive value for postoperative recurrence of OC patients.

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