VAPB在肝癌中的表达与促增殖作用研究-东南大学学报医学版

VAPB在肝癌中的表达与促增殖作用研究

单位：空军军医大学第二附属医院疼痛科, 陕西西安 710038

分类号：R735.7

出版年·卷·期（页码）：2022·41·第五期（637-643）

摘要：

目的：研究囊泡相关膜蛋白相关蛋白B （VAPB）在肝癌中的表达及其在肝癌细胞增殖与凋亡中的调控作用。方法：（1）分别用生物信息学分析与免疫组化实验，分析VAPB在肝癌癌组织与癌旁组织中的表达。（2）用实时荧光定量PCR （RT-PCR）与蛋白质印迹实验，检测肝癌细胞株与正常肝细胞株中VAPB的表达。（3）分别用MTS细胞活力实验与EdU细胞增殖实验，检测通过转染siRNA下调VAPB表达对肝癌细胞生长的影响。（4）用流式细胞仪检测通过转染siRNA下调VAPB对肝癌细胞凋亡的影响。结果：（1）与癌旁组织相比，VAPB在肝癌组织中的表达显著升高。（2）与正常肝细胞株相比，VAPB在4株肝癌细胞株中的表达均显著上调。（3）下调VAPB表达后，肝癌细胞的活力与增殖能力均被显著抑制。（4）下调VAPB表达诱导了肝癌细胞的凋亡。结论：肝癌组织与细胞中VAPB表达异常升高，VAPB可促进体外肝癌细胞的活力与增殖能力，同时抑制肝癌细胞的凋亡。

Objective: To explore the expression and biological roles of vesicle-associated membrane protein-associated protein B(VAPB) in the regulation of liver cancer growth. Methods: (1) Bioinformatic analysis and immunohistochemistry staining assay were applied to evaluate the expression of VAPB in tumor and non-tumor tissues of liver. (2) VAPB expression was detected in tumor and non-tumor cell lines of liver by RT-PCR and Western blotting. (3) Cell viability and proliferation were determined by MTS cell viability and EdU (5-Ethynyl-2'-deoxyuridine) incorporation assays upon VAPB down-regulated expression by siRNA. (4) Cell apoptosis was also determined by flow cytometry upon VAPB down-regulated expression by siRNA. Results: (1) VAPB expression was significantly higher in liver tumor tissues than that in normal liver tissues. (2) VAPB expressions were also significantly higher in liver cancer cell lines than those in normal liver cell lines. (3) Cell viability and proliferation were markedly decreased upon VAPB down-regulated expression in liver cancer cells. (4) VAPB down-regulated expression induced cell apoptosis in liver cancer cells. Conclusion: VAPB overexpression promotes cell viability and proliferation, while suppresses cell apoptosis in liver cancer tissues and cells.

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