金荞麦对急性溃疡性肠炎小鼠肠黏膜的保护作用及其机制的研究-东南大学学报医学版

金荞麦对急性溃疡性肠炎小鼠肠黏膜的保护作用及其机制的研究

单位：湖北省妇幼保健院急诊科, 湖北武汉 430000

分类号：R574.1;R282.71

出版年·卷·期（页码）：2022·41·第三期（421-426）

摘要：

目的：探金荞麦对溃疡性结肠炎（ulcerative colitis，UC）动物模型肠黏膜的保护作用及对IL-6/STAT3转导途径的调控作用。方法：将45只小鼠分为对照组、UC组、UC+金荞麦片组（每组15只）。利用3%的葡聚糖硫酸钠诱导UC模型。UC+金荞麦片组通过灌胃行金荞麦片干预。检测各组小鼠结肠长度、疾病活动指数、肠黏膜通透性、组织中炎症细胞因子水平、紧密连接蛋白表达水平以及IL-6、STAT3的转录和蛋白表达水平。结果：UC组疾病活动指数，IL-1β水平，TNF-α水平，肠黏膜通透性，IL-6、STAT3 mRNA和蛋白表达水平显著高于对照组（P<0.05），ZO-1、Occludin蛋白表达水平低于对照组（P<0.005），结肠长度显著短于对照组（P<0.05）。UC+金荞麦片组疾病活动指数，IL-1β水平，TNF-α水平、肠黏膜通透性，IL-6、STAT3 mRNA和蛋白表达水平显著低于UC组（P<0.05），ZO-1、Occludin蛋白表达水平高于UC组（P<0.05），结肠长度显著长于UC组（P<0.05）。结论：金荞麦可通过抑制IL-6/STAT3通路抑制炎症反应，保护肠黏膜屏障，缓解UC。

Objective: To explore the protective effect of Fagopyrum dibotrys on the animal model of ulcerative colitis(UC) and the regulation of IL-6/STAT3 transduction pathway. Methods: Forty-five mice were divided into control group, UC group, and UC+Fagopyrum dibotrys group(n=15). Use 3% dextransulfate sodium salt(DSS) was applied to induce UC model. Fagopyrum dibotrys was intervened by gavage. The colon length, disease activity index, intestinal mucosal permeability, inflammatory cytokine levels in tissues, tight junction protein expression levels, IL-6 and STAT3 transcription and protein expression levels were detected. Results: The disease activity index, IL-1β, TNF-α, intestinal mucosal permeability, IL-6 and STAT3 mRNA and protein levels in the UC group were significantly higher than those in the control group, ZO-1 and Occludin protein, and the colon length were significantly lower than those in the control Group(P<0.05). The disease activity index, IL-1β, TNF-α, intestinal mucosal permeability, IL-6 and STAT3 mRNA and protein levels in the UC+Fagopyrum dibotrys group were significantly lower than those in the UC group, ZO-1 and Occludin protein, and the colon length were significantly higher than those in the UC group(P<0.05). Conclusion: Fagopyrum dibotrys can inhibit IL-6/STAT3 pathway to inhibit inflammatory response, protect the intestinal mucosal barrier and relieve UC.

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