肺炎支原体肺炎儿童血清miR-16的表达水平及其对肺炎支原体免疫的作用-东南大学学报医学版

肺炎支原体肺炎儿童血清miR-16的表达水平及其对肺炎支原体免疫的作用

单位：东方市人民医院儿科, 海南东方 572600

分类号：R725.6

出版年·卷·期（页码）：2022·41·第三期（345-351）

摘要：

目的：探究血清miR-16在肺炎支原体肺炎（MPP）患儿中的表达水平及其对肺炎支原体免疫的作用。方法：2020年1月至2021年3月选取本院收治的200例MPP患儿为研究组，其中急性期组90例，恢复期组110例。此外，同期招募100例健康儿童作为对照组。检测血清miR-16、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-8、IL-10、IL-13、免疫球蛋白（Ig）G、IgA、补体（C3、CH50）和T淋巴细胞亚群（CD3⁺、CD4⁺、CD8⁺、CD4⁺/CD8⁺）的表达水平。采用受试者工作特征（ROC）曲线确定miR-16对MPP的诊断价值。结果：MPP患儿血清miR-16水平较对照组降低（P<0.05），且急性期组低于恢复期组（P<0.05）。经Spearman秩相关分析，MPP患儿或急性期组MPP患儿血清miR-16水平与TNF-α、IL-8、补体C3和CH50水平呈负相关（均r<0，P<0.05），且与IL-10、IgG、IgA水平及CD4⁺/CD8⁺呈正相关（均r>0，P<0.05）。但是对于恢复期组患儿，只有血清IL-8、IgG、补体C3水平与血清miR-16水平有关（P<0.05）。经ROC曲线分析，血清miR-16用于MPP诊断的曲线下面积（AUC）为0.758（95%CI：0.687~0.829，P<0.05），用于区分MPP急性期和恢复期患儿的AUC为0.784（95%CI：0.732~0.837，P<0.05）。结论：MPP患儿血清miR-16水平普遍降低，且低水平血清miR-16在一定程度上反映了急性期患儿炎症和免疫紊乱状态。

Objective: To investigate the expression of serum miR-16 in children with Mycoplasma pneumoniae pneumonia(MPP) and its effect on the immunity of Mycoplasma pneumoniae. Methods: From January 2020 to March 2021, children with MPP admitted to our hospital were selected as study group, including 90 cases in the acute stage and 110 cases in recovery. In addition, 100 healthy children were recruited as control group during the same period. The expression levels of miR-16, tumor necrosis factor-α(TNF-α), interleukin(IL)-8, IL-10, IL-13, immunoglobulin(Ig) G, IgA, complement(C3, CH50) and T lymphocyte subsets(CD3⁺, CD4⁺, CD8⁺, CD4⁺/CD8⁺) were detected. Receiver operating characteristic(ROC) curve was used to determine the diagnostic value of miR-16 for MPP. Results: The level of serum miR-16 decreased in MPP children compared with the control group(P<0.05). The serum level of miR-16 in acute MPP patients was also lower than that in recovery(P<0.05). Spearman rank correlation analysis showed that serum miR-16 was negatively correlated with the levels of TNF-α, IL-8, complement C3 and CH50 in all children with MPP or in the acute stage group(all r<0, P<0.05), and positively correlated with IL-10, IgG, IgA, CD4⁺/CD8⁺(all r>0, P<0.05). However, only serum IL-8, IgG and complement C3 levels were related to serum miR-16 levels in those in recovery(P<0.05). According to ROC curve analysis, the area under the curve(AUC) of serum miR-16 for MPP diagnosis was 0.758(95%CI:0.687-0.829, P<0.05), and the AUC of serum miR-16 for distinguishing between acute and recovery stage MPP was 0.784(95%CI:0.732-0.837, P<0.05). Conclusion: Serum miR-16 in children with MPP generally decreases, and the low level of serum miR-16 may reflect the state of inflammation and immune disorder in children with acute stage.

参考文献：

[1] TSAI T A,TSAI C K,KUO K C,et al.Rational stepwise approach for Mycoplasma pneumoniae pneumonia in children[J].J Microbiol Immunol Infect,2021,54(4):557-565.
[2] 梁伟,管世鹤,周强,等.儿童社区获得性肺炎支原体感染的流行病学分析[J].国际检验医学杂志,2018,39(21):2708-2711.
[3] 谢明,邓葵珍,谭晴心,等.外周血血清sTREM-1、Treg/Th17、miR-99b与耐多药结核病相关性及联合预测疗效的ROC分析[J].现代医学,2021,49(10):1176-1182.
[4] CHEN Y,SHAN T,QU H,et al.Inhibition of miR-16 ameliorates inflammatory bowel disease by modulating Bcl-2 in mouse models[J].J Surg Res,2020,253:185-192.
[5] WANG M,LI J,CAI J,et al.Overexpression of microRNA-16 alleviates atherosclerosis by inhibition of inflammatory pathways[J].Biomed Res Int,2020,2020:8504238.
[6] ZHANG K,HAN Y,ZHAO Y,et al.Upregulated gga-miR-16-5p inhibits the proliferation cycle and promotes the apoptosis of MG-infected DF-1 cells by repressing PIK3R1-mediated the PI3K/Akt/NF-kappaB pathway to exert anti-inflammatory effect[J].Int J Mol Sci,2019,20(5):1036-1051.
[7] 中华中医药学会儿童肺炎联盟.儿童肺炎支原体肺炎中西医结合诊治专家共识(2017年制定)[J].中国实用儿科杂志,2017,32(12):881-885.
[8] GALVÁN-ROMÁN J M,LANCHO-SANCHEZ Á,LUQUERO-BUENO S,et al.Usefulness of circulating microRNAs miR-146a and miR-16-5p as prognostic biomarkers in community-acquired pneumonia[J].PLoS One,2020,15(10):e0240926.
[9] FAN H,LU B,YANG D,et al.Distribution and expression of IL-17 and related cytokines in children with Mycoplasma pneumoniae pneumonia[J].Jpn J Infect Dis,2019,72(6):387-393.
[10] CHAI Q L.Effect of azithromycin combined with licorzinc therapy on inflammatory response and immune response in children with Mycoplasma pneumonia[J].J Hainan Med Univ,2017,23(14):62-65.
[11] WANG T,DING Y,SHI Y,et al.Changes on serum myocardial enzyme of Mycoplasma pneumoniae pneumonia and clinical significance[J].Biomed Res,2017,28:9593-9595.
[12] JIANG Z,LI S,ZHU C,et al.Mycoplasma pneumoniae infections:pathogenesis and vaccine development[J].Pathogens,2021,10(2):119.
[13] 代树栋,李金涛.支原体肺炎合并哮喘患儿血清维生素D水平与T淋巴细胞亚群及肺功能的相关性研究[J].现代医学,2019,47(6):640-645.
[14] ZHU T,LIN Z,HAN S,et al.Low miR-16 expression induces regulatory CD4⁺ NKG2D⁺ T cells involved in colorectal cancer progression[J].Am J Cancer Res,2021,11(4):1540-1556.
[15] JIA X,LIU H,XU C,et al.MiR-15a/16-1 deficiency induces IL-10-producing CD19⁺ TIM-1⁺ cells in tumor microenvironment[J].J Cell Mol Med,2019,23(2):1343-1353.
[16] LI Z,JIANG W,WU G,et al.miR-16 inhibits hyperoxia-induced cell apoptosis in human alveolar epithelial cells[J].Mol Med Rep,2018,17(4):5950-5957.

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