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窒息新生儿生后一周内肠道菌群产气差异分析
作者:张华婷  尹迪  王雪芳  管坤玉  龚小慧  胡勇 
单位:上海交通大学附属儿童医院, 上海市儿童医院 新生儿科, 上海 200062
关键词:新生儿窒息 肠道菌群 体外发酵 产气 
分类号:R722.12
出版年·卷·期(页码):2022·41·第二期(208-214)
摘要:

目的:比较窒息新生儿与非窒息新生儿粪便肠道菌群对不同碳水化合物产气的差异,探讨其临床意义。方法:选取窒息新生儿20例,同时选取20例非窒息新生儿作为对照,收集入组患儿出生后第1、第7天粪便样本,用以乳糖(LAT)、低聚果糖(FOS)、母乳低聚糖(FL-2)和低聚半乳糖(GOS)为主要碳源的培养基进行体外发酵,采用肠道体外气体分析仪测定发酵后产气总量及主要成分二氧化碳(CO2)、氢气(H2)、甲烷(CH4)、硫化氢(H2S)的含量,比较各组差异。结果:窒息组患儿生后第1、第7天粪便菌群发酵GOS产气总量均显著高于对照组,粪便发酵 FOS、GOS、LAT后产CO2量均显著高于对照组(P<0.05),生后第 1 天粪便发酵4种底物后产CH4量均显著高于对照组(P<0.05)。结论:窒息新生儿肠道产气量显著高于对照组婴儿,其中CO2含量明显高于对照组。受不同碳源影响,增加母乳低聚糖喂养或许能减少气体产生。肠道CH4产生量明显高于对照组,提示早期CH4菌定植可能,参与新生儿早期胃肠功能紊乱的发生发展。

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