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安石榴甙对骨肉瘤细胞增殖、迁移和侵袭的影响及其机制研究
作者:苏乙花  汪云鑫  陈学武 
单位:海南省中医院 肿瘤科, 海南 海口 570203
关键词:安石榴甙 骨肉瘤 增殖 侵袭 迁移 
分类号:R738
出版年·卷·期(页码):2022·41·第二期(170-175)
摘要:

目的:探讨安石榴甙(PUN)对骨肉瘤(OS)细胞增殖、迁移和侵袭的影响及其可能机制。方法:选取OS的U2OS和MG63细胞,用不同浓度PUN处理,分别设置为不加PUN的对照组和50、75、100 μmol·L-1 PUN组。用结晶紫法检测细胞增殖能力,细胞划痕愈合实验检测细胞迁移,Transwell小室法检测细胞侵袭和迁移,蛋白质印迹法检测分泌型糖蛋白/β-连锁蛋白通路相关蛋白(β-连环蛋白、c-Myc蛋白、细胞周期蛋白D1)和上皮间质转化(EMT)相关蛋白[E钙黏蛋白、N钙黏蛋白、波形纤维蛋白、锌指蛋白转录因子(ZEB1、Snial、Twist)]表达水平。结果:不同剂量PUN均可抑制U2OS和MG63细胞的增殖、侵袭和迁移,并且存在剂量依赖性,剂量越大,抑制作用越强(P<0.05)。与对照组及50 μmol·L-1组比较,75 μmol·L-1及100 μmol·L-1组N钙黏蛋白、波形纤维蛋白及ZEB1、Snial、Twist蛋白表达水平较低,而E钙黏蛋白表达水平较高(P<0.05)。与对照组比较,75 μmol·L-1组及100 μmol·L-1组β-连环蛋白、c-Myc基因、细胞周期蛋白D1蛋白表达水平较低(P<0.05)。结论:PUN可能通过抑制分泌型糖蛋白/β-连锁蛋白通路和EMT而抑制OS瘤细胞的增殖、侵袭、迁移。

Objective: To explore the effect of punica granaside(PUN) on the proliferation, migration and invasion of osteosarcoma(OS) cells and its possible mechanism. Methods: Osteosarcoma U2OS and MG63 cells were selected as the research objects. The two tumor cell lines were intervened with different concentrations of PUN(0, 50, 75, and 100 μmol·L-1). Crystal violet method was employed to detect cell proliferation, cell scratch healing test was used to detect cell migration, and Transwell chamber method was used to detect cell invasion and migration. Western blot was used to determine Wnt/β-catenin pathway related proteins(β-catenin, c-Myc, Cyclin D1) and epithelial-mesenchymal transition(EMT) related proteins[E-cadherin, N-cadherin, vimentin, zinc finger protein transcription factors(ZEB1, Snial, Twist)] expression levels. Results: Different doses of PUN could inhibit the proliferation, invasion and migration of U2OS and MG63 cells with a dose-dependent manner. The higher the dose was, the stronger the inhibitory effect(P<0.05). Compared with the control group and 50 μmol·L-1 group, the N-cadherin, vimentin, ZEB1, Snial, and Twist protein expression levels were lower in the 75 μmol·L-1 group and 100 μmol·L-1 group, while the E-cadherin protein expression level was higher(P<0.05). Compared with the control group, the expression levels of β-catenin, c-Myc and Cyclin D1 in the 75 μmol·L-1 group and 100 μmol·L-1 group were lower(P<0.05). Conclusion: PUN inhibits the proliferation, invasion and migration of OS tumor cells by inhibiting the Wnt/β-catenin pathway and EMT.

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