Objective: To explore the effect of punica granaside(PUN) on the proliferation, migration and invasion of osteosarcoma(OS) cells and its possible mechanism. Methods: Osteosarcoma U2OS and MG63 cells were selected as the research objects. The two tumor cell lines were intervened with different concentrations of PUN(0, 50, 75, and 100 μmol·L-1). Crystal violet method was employed to detect cell proliferation, cell scratch healing test was used to detect cell migration, and Transwell chamber method was used to detect cell invasion and migration. Western blot was used to determine Wnt/β-catenin pathway related proteins(β-catenin, c-Myc, Cyclin D1) and epithelial-mesenchymal transition(EMT) related proteins[E-cadherin, N-cadherin, vimentin, zinc finger protein transcription factors(ZEB1, Snial, Twist)] expression levels. Results: Different doses of PUN could inhibit the proliferation, invasion and migration of U2OS and MG63 cells with a dose-dependent manner. The higher the dose was, the stronger the inhibitory effect(P＜0.05). Compared with the control group and 50 μmol·L-1 group, the N-cadherin, vimentin, ZEB1, Snial, and Twist protein expression levels were lower in the 75 μmol·L-1 group and 100 μmol·L-1 group, while the E-cadherin protein expression level was higher(P＜0.05). Compared with the control group, the expression levels of β-catenin, c-Myc and Cyclin D1 in the 75 μmol·L-1 group and 100 μmol·L-1 group were lower(P＜0.05). Conclusion: PUN inhibits the proliferation, invasion and migration of OS tumor cells by inhibiting the Wnt/β-catenin pathway and EMT.
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