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细胞薄片组织工程及其临床转化的研究进展
作者:俞杭1  王经琳1 2  任昊桢1 2  施晓雷1 2 
单位:1. 南京中医药大学 中西医结合鼓楼临床医学院, 江苏 南京 210046;
2. 南京大学医学院附属鼓楼医院 普外科, 江苏 南京 210008
关键词:细胞薄片 组织工程 组织再生 细胞移植 温度响应 综述 
分类号:R459.9
出版年·卷·期(页码):2022·41·第一期(148-154)
摘要:

细胞移植是一种很有前景的治疗受损组织或器官并促进其再生的手段。常规的细胞疗法是直接将细胞注射至受损组织,但其将细胞输送到靶部位的效率有限。"细胞薄片组织工程"是一种新兴的无支架组织工程技术,利用温度敏感型聚合物制造出的组织状细胞单层(即"细胞薄片")可以被输送到受损部位,并且不需要支架,定植效果强大。目前,细胞薄片组织工程已经被应用于多种器官的组织再生。因此,本综述从细胞薄片的制备以及其在临床应用等多个方面进行系统的归纳和阐述。

参考文献:

[1] LANGER R, VACANTI J P.Tissue engineering[J].Science, 1993, 260(5110):920-926.
[2] GUO R, WAN F, MORIMATSU M, et al.Cell sheet formation enhances the therapeutic effects of human umbilical cord mesenchymal stem cells on myocardial infarction as a bioactive material[J].Bioactive Materials, 2021, 6(9):2999-3012.
[3] GUÉRIN L P, LE-BEL G, DESJARDINS P, et al.The human tissue-engineered cornea(hTEC):recent progress[J].International Journal of Molecular Sciences, 2021, 22(3):1291.
[4] COSSU G, BIRCHALL M, BROWN T, et al.Lancet commission:stem cells and regenerative medicine[J].The Lancet, 2018, 391(10123):883-910.
[5] KWON H, BROWN W E, LEE C A, et al.Surgical and tissue engineering strategies for articular cartilage and meniscus repair[J].Nature Reviews Rheumatology, 2019, 15(9):550-570.
[6] YANG J, YAMATO M, KOHNO C, et al.Cell sheet engineering:recreating tissues without biodegradable scaffolds[J].Biomaterials, 2005, 26(33):6415-6422.
[7] ASHTARI K, NAZARI H, KO H, et al.Electrically conductive nanomaterials for cardiac tissue engineering[J].Advanced Drug Delivery Reviews, 2019, 144:162-179.
[8] SJÖQVIST S, ISHIKAWA T, SHIMURA D, et al.Exosomes derived from clinical-grade oral mucosal epithelial cell sheets promote wound healing[J].Journal of Extracellular Vesicles, 2019, 8(1):1565264.
[9] KASAI Y, TAKAGI R, KOBAYASHI S, et al.A stable protocol for the fabrication of transplantable human oral mucosal epithelial cell sheets for clinical application[J].Regenerative Therapy, 2020, 14:87-94.
[10] IWATA T, YAMATO M, WASHIO K, et al.Periodontal regeneration with autologous periodontal ligament-derived cell sheets-a safety and efficacy study in ten patients[J].Regenerative Therapy, 2018, 9:38-44.
[11] VAQUETTE C, SAIFZADEH S, FARAG A, et al.Periodontal tissue engineering with a multiphasic construct and cell sheets[J].Journal of Dental Research, 2019, 98(6):673-681.
[12] GIULIANI C.The flavonoid quercetin induces AP-1 activation in FRTL-5 thyroid cells[J].Antioxidants, 2019, 8(5):112.
[13] MIRDAMADI E S, KALHORI D, ZAKERI N, et al.Liver tissue engineering as an emerging alternative for liver disease treatment[J].Tissue Engineering Part B:Reviews, 2020, 26(2):145-163.
[14] THEVENOT P, NAIR A, DEY J, et al.Method to analyze three-dimensional cell distribution and infiltration in degradable scaffolds[J].Tissue Engineering Part C:Methods, 2008, 14(4):319-331.
[15] DVIR-GINZBERG M, GAMLIELI-BONSHTEIN I, AGBARIA R, et al.Liver tissue engineering within alginate scaffolds:effects of cell-seeding density on hepatocyte viability, morphology, and function[J].Tissue Engineering, 2003, 9(4):757-766.
[16] ZHANG K, CHEN J.The regulation of integrin function by divalent cations[J].Cell Adhesion & Migration, 2012, 6(1):20-29.
[17] TAKEICHI M, ATSUMI T, YOSHIDA C, et al.Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites[J].Developmental Biology, 1981, 87(2):340-350.
[18] CHEN F, ZHOU Y, BARNABAS S T, et al.Engineering tubular bone constructs[J].Journal of Biomechanics, 2007, 40:S73-S79.
[19] RAYATPISHEH S, HEATH D E, SHAKOURI A, et al.Combining cell sheet technology and electrospun scaffolding for engineered tubular, aligned, and contractile blood vessels[J].Biomaterials, 2014, 35(9):2713-2719.
[20] ZURINA I M, PRESNIAKOVA V S, BUTNARU D V, et al.Tissue engineering using a combined cell sheet technology and scaffolding approach[J].Acta Biomaterialia, 2020, 113:63-83.
[21] OKANO T, YAMADA N, SAKAI H, et al.A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropylacrylamide)[J].Journal of Biomedical Materials Research, 1993, 27(10):1243-1251.
[22] AKIYAMA Y, KIKUCHI A, YAMATO M, et al.Accelerated cell-sheet recovery from a surface successively grafted with polyacrylamide and poly(N-isopropylacrylamide)[J].Acta Biomaterialia, 2014, 10(8):3398-3408.
[23] NAGAMOTO Y, TAKAYAMA K, OHASHI K, et al.Transplantation of a human iPSC-derived hepatocyte sheet increases survival in mice with acute liver failure[J].Journal of Hepatology, 2016, 64(5):1068-1075.
[24] FORGHANI A, KRIEGH L, HOGAN K, et al.Fabrication and characterization of cell sheets using methylcellulose and PNIPAAm thermoresponsive polymers:a comparison study:cell sheets using mc and pnipaam thermoresponsive polymers[J].Journal of Biomedical Materials Research Part A, 2017, 105(5):1346-1354.
[25] BENCHAPRATHANPHORN K, SAKULAUE P, SIRIWATWECHAKUL W, et al.Preparation and characterization of human keratinocyte-fibroblast cell sheets constructed using PNIAM-co-AM grafted surfaces for burn wound healing[J].Journal of Materials Science:Materials in Medicine, 2020, 31(12):1609-1614.
[26] IDE T, NISHIDA K, YAMATO M, et al.Structural characterization of bioengineered human corneal endothelial cell sheets fabricated on temperature-responsive culture dishes[J].Biomaterials, 2006, 27(4):607-614.
[27] TATSUMI K, OKANO T.Hepatocyte transplantation:cell sheet technology for liver cell transplantation[J].Current Transplantation Reports, 2017, 4(3):184-192.
[28] OHASHI K, MATSUBARA Y, TATSUMI K, et al.Cell therapy using adipose-derived stem cells for chronic liver injury in mice[J].Cell Medicine, 2012, 3(1-3):113-119.
[29] ITABA N, NODA I, OKA H, et al.Hepatic cell sheets engineered from human mesenchymal stem cells with a single small molecule compound IC-2 ameliorate acute liver injury in mice[J].Regenerative Therapy, 2018, 9:45-57.
[30] FIORETTA E S, MOTTA S E, LINTAS V, et al.Next-generation tissue-engineered heart valves with repair, remodelling and regeneration capacity[J].Nature Reviews Cardiology, 2021, 18(2):92-116.
[31] HARAGUCHI Y, SHIMIZU T, YAMATO M, et al.Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation[J].Biomaterials, 2006, 27(27):4765-4774.
[32] LEVENBERG S, ROUWKEMA J, MACDONALD M, et al.Engineering vascularized skeletal muscle tissue[J].Nature Biotechnology, 2005, 23(7):879-884.
[33] TAKAHASHI H, OKANO T.Thermally-triggered fabrication of cell sheets for tissue engineering and regenerative medicine[J].Advanced Drug Delivery Reviews, 2019, 138:276-292.
[34] TSAI R J, LI L M, CHEN J K.Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells[J].The New England Journal of Medicine, 2000, 343(2):86-93.
[35] NISHIDA K, YAMATO M, HAYASHIDA Y, et al.Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface[J].Transplantation, 2004, 77(3):379-385.
[36] NISHIDA K, YAMATO M, HAYASHIDA Y, et al.Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium[J].New England Journal of Medicine, 2004, 351(12):1187-1196.
[37] CUI Z, LEE B H, PAUKEN C, et al.Degradation, cytotoxicity, and biocompatibility of NIPAAm-based thermosensitive, injectable, and bioresorbable polymer hydrogels[J].Journal of Biomedical Materials Research Part A, 2011, 98A(2):159-166.
[38] OHKI T, YAMATO M, OTA M, et al.Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets[J].Gastroenterology, 2012, 143(3):582-588.e2.
[39] YAMAGUCHI N, ISOMOTO H, KOBAYASHI S, et al.Oral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation[J].Scientific Reports, 2017, 7(1):17460.
[40] WASHIO K, IWATA T, MIZUTANI M, et al.Assessment of cell sheets derived from human periodontal ligament cells:a pre-clinical study[J].Cell and Tissue Research, 2010, 341(3):397-404.
[41] OKA M, SEKIYA S, SAKIYAMA R, et al.Hepatocyte growth factor-secreting mesothelial cell sheets suppress progressive fibrosis in a rat model of CKD[J].Journal of the American Society of Nephrology, 2019, 30(2):261-276.
[42] IMAFUKU A, OKA M, MIYABE Y, et al.Rat mesenchymal stromal cell sheets suppress renal fibrosis via microvascular protection[J].Stem Cells Translational Medicine, 2019, 8(12):1330-1341.
[43] HAYASHIDA Y, NISHIDA K, YAMATO M, et al.Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated Ex Vivo on a temperature-responsive culture surface[J].Investigative Opthalmology & Visual Science, 2005, 46(5):1632-1639.
[44] MANDAI M, WATANABE A, KURIMOTO Y, et al.Autologous induced stem-cell-derived retinal cells for macular degeneration[J].New England Journal of Medicine, 2017, 376(11):1038-1046.
[45] ASAKAWA N, SHIMIZU T, TSUDA Y, et al.Pre-vascularization of in vitro three-dimensional tissues created by cell sheet engineering[J].Biomaterials, 2010, 31(14):3903-3909.
[46] SAKAI Y, YAMANOUCHI K, OHASHI K, et al.Vascularized subcutaneous human liver tissue from engineered hepatocyte/fibroblast sheets in mice[J].Biomaterials, 2015, 65:66-75.
[47] SHIMIZU T, SEKINE H, YANG J, et al.Polysurgery of cell sheet grafts overcomes diffusion limits to produce thick, vascularized myocardial tissues[J].The FASEB Journal, 2006, 20(6):708-710.
[48] AYALA P, CAVES J, DAI E, et al.Engineered composite fascia for stem cell therapy in tissue repair applications[J].Acta Biomaterialia, 2015, 26:1-12.
[49] CHUAH Y J, TAN J R, WU Y, et al.Scaffold-Free tissue engineering with aligned bone marrow stromal cell sheets to recapitulate the microstructural and biochemical composition of annulus fibrosus[J].Acta Biomaterialia, 2020, 107:129-137.

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