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急性冠脉综合征患者大颗粒HDL结合miR-33介导HDL趋炎化转变
作者:赵晓静  刘树理  张琳 
单位:保定市第二医院 老年病科, 河北 保定 071000
关键词:急性冠脉综合征 失功能高密度脂蛋白 微小RNA-33 急性炎症 动脉粥样硬化 
分类号:R543.3;R446.1
出版年·卷·期(页码):2022·41·第一期(10-15)
摘要:

目的:探讨急性冠脉综合征(ACS)患者高密度脂蛋白(HDL)结合miR-33水平是否影响HDL功能。方法:纳入48例ACS患者为ACS组,52例健康体检者为对照组。分离和纯化HDL大中小颗粒亚组分后,实时荧光定量PCR法检测各亚组分及血浆miR-33表达。体外实验分组如下:阴性对照组、rHDL组、HDL大颗粒(L-HDL)组、HDL中颗粒组、HDL小颗粒组和L-HDL+anti-miR33组。3H标记胆固醇完成细胞内胆固醇流出实验,Luminex 200试剂盒检测炎症因子在THP-1泡沫细胞中的表达。结果:miR-33在ACS患者血浆及HDL各亚组分中均明显增加,特别是L-HDL。ACS患者HDL各亚组分的细胞胆固醇流出能力及抗炎能力明显受损。抑制miR-33和HDL的结合后,L-HDL的胆固醇流出能力及抗炎能力均有所恢复。结论:ACS急性炎症状态下,L-HDL结合miR-33水平上调,介导HDL抗动脉粥样硬化功能受损。

Objective: To investigate whether the combination of HDL and miR-33 in patients with acute coronary syndromes(ACS) could impair function of HDL. Methods: 48 ACS patients were enrolled as the ACS group, and 52 healthy subjects as the control group. HDL was separated and purified as large, medium and small particles from the two groups. Real-time fluorescent quantitative PCR was performed to detect the expression levels of each sub-fraction and plasma miR-33. In vitro, experiment groups included:negative control group, rHDL group, large HDL particle(L-HDL) group, medium HDL particle group, small HDL particle group and L-HDL+anti-miR33 group. 3H labeled cholesterol was used to detect intracellular cholesterol efflux and the Luminex 200 kit was used to detect the expression of inflammatory factors in THP-1 foam cells. Results: The study showed that the levels of miR-33 in the plasma and each sub-fraction of HDL of ACS patients were overall increased significantly, especially in L-HDL. Compared with the control group, the cellular cholesterol efflux capacity and anti-inflammatory capacity of each sub-fraction of HDL in ACS patients were significantly impaired. After inhibiting the combination between miR-33 and HDL, the cholesterol efflux ability and anti-inflammatory ability of L-HDL were partly restored. Conclusion: In the acute inflammatory state of ACS, the level of miR-33 combined with HDL was up-regulated, leading to the dysfunctional HDL which antiatherogenic function has been impaired.

参考文献:

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