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支气管哮喘患儿肺泡灌洗液miR-98-5p及靶蛋白STAT3的表达与气道炎症的相关性分析
作者:杜明红1  周云1  刘成军2 
单位:1. 重庆市涪陵区妇幼保健院 儿科, 重庆 408000;
2. 重庆医科大学附属儿童医院 重症医学科, 重庆 400000
关键词:支气管哮喘 miR-98-5p 信号转导及转录激活因子3 气道炎症 肺功能 
分类号:R725.6
出版年·卷·期(页码):2021·40·第五期(599-605)
摘要:

目的:探讨支气管哮喘患儿肺泡灌洗液(BALF)中miR-98-5p及靶蛋白STAT3表达水平与气道炎症的相关性。方法:选取2017年1月至2020年5月于重庆市涪陵区妇幼保健院就诊的120例支气管哮喘患儿作为哮喘组,包含72例急性发作期患儿(BA-E组)和48例缓解期患儿(BA-R组);纳入20例无支气管哮喘患儿为对照组。收集BALF,用实时荧光定量PCR法检测其中miR-98-5p相对表达量,采用酶联免疫吸附法检测STAT3和气道炎症因子(IL-6、IL-10、IL-13、TNF-α、总IgE),另外检测哮喘患儿肺功能和FeNO含量。结果:与对照组相比,哮喘患儿BALF中miR-98-5p相对表达量降低,STAT3水平升高,且BA-E组患儿miR-98-5p和STAT3水平变化更明显(P<0.05)。经TargetScan在线软件和双荧光素酶报告基因分析显示,miR-98-5p存在与STAT3 mRNA相结合的互补序列。与BA-R组相比,BA-E组FEV1、FEV1/FVC以及BALF中IL-10水平降低(P<0.001),同时FeNO和BALF中IL-6、IL-13、TNF-α、IgE水平均增高(均P<0.05)。经Pearson分析,哮喘患儿BALF中miR-98-5p与STAT3、IL-6、IL-13、TNF-α、IgE呈负相关(均r<0,P<0.001),与IL-10水平则呈正相关(r=0.288,P=0.001);而STAT3水平与IL-6、IL-13、TNF-α、IgE均呈正相关(均r>0,P<0.001),与IL-10水平则呈负相关(r=-0.285,P<0.001)。结论:支气管哮喘患儿BALF中miR-98-5p相对表达量降低,同时STAT3蛋白水平升高,且两者与气道炎症有关。

Objective: To explore the correlation between the expression levels of miR-98-5p and the target protein STAT3 in alveolar lavage fluid of children with bronchial asthma and airway inflammation. Methods: A total of 120 children with bronchial asthma who were hospitalized or outpatient in our hospital from January 2017 to May 2020 were selected into asthma group, including 72 children in acute onset (BA-E group) and 48 children in remission (BA-R group), additional 20 children without bronchial asthma into control group. The bronchoalveolar lavage fluid samples (BALF) were collected and detected for miR-98-5p, STAT3 and airway inflammatory factors (IL-6, IL-10, IL-13, TNF-α, IgE). The lung function and the content of FeNO were also detected. Results: Compared with the control group, the relative expression of miR-98-5p in the BALF samples of children with asthma in the two sub-groups decreased, and the level of STAT3 increased, and the changes in the levels of miR-98-5p and STAT3 in the BA-E group were more obvious (P<0.05). TargetScan online software and dual luciferase reporter gene analysis proved that miR-98-5p has a complementary sequence binding to STAT3 mRNA. Compared with children in the BA-R group, the levels of IL-10 in the FEV1, FEV1/FVC and BALF samples in the BA-E group decreased, while the levels of IL-6, IL-13, TNF-α, and IgE in FeNO and BALF increased (all P<0.001). According to Pearson analysis, miR-98-5p was negatively correlated with STAT3, IL-6, IL-13, TNF-α, and IgE(all r<0,P<0.001). There was a positive correlation with IL-10 level (r=0.288,P=0.001); while the STAT3 level in BALF was positively correlated with IL-6, IL-13, TNF-α, and IgE(all r>0,P<0.001), and was negatively correlated with IL-10 level (r=-0.285, P<0.001). Conclusion: The relative expression of miR-98-5p in BALF of asthmatic children decreases, while the level of STAT3 protein increases, which is related to airway inflammation.

参考文献:

[1] 殷勇,卢燕鸣,乔荆,等.基层儿童支气管哮喘临床诊治策略——上海市浦东新区/奉贤区专家建议(附同行评议)[J].中国全科医学,2020,23(6):633-643,648.
[2] 唐文慧,张伟,李春梅.布地奈德福莫特罗联合孟鲁司特钠治疗支气管哮喘的疗效及对炎症因子水平的影响[J].医学综述,2020,26(12):2493-2497.
[3] 唐晋,周靖,陈雪梅,等.miR-98-5p通过靶向调控TRAF6表达促进肺泡巨噬细胞M2表型分化以保护脓毒症引起的急性肺损伤[J].免疫学杂志,2020,36(8):645-654.
[4] 刘慧琳,魏敏,王国霞.miR-98-5p通过降低STAT3水平促进A549细胞凋亡并抑制其侵袭和迁移[J].细胞与分子免疫学杂志,2018,34(6):522-527.
[5] CHONG L,LIU L,ZHU L L,et al.Expression levels of predominant adipokines and activations of STAT3,STAT6 in an experimental mice model of obese asthma[J].Iran J Allergy Asthma Immunol,2019,18(1):62-71.
[6] 中华医学会儿科学分会呼吸学组.儿童支气管哮喘诊断与防治指南(2016年版)[J].中华儿科杂志,2016,54(3):167-181.
[7] LICARI A,MANTI S,CASTAGNOLI R,et al.Measuring inflammation in paediatric severe asthma:biomarkers in clinical practice[J].Breathe(Sheff),2020,16(1):190301.
[8] 易拉,魏颖,崔洁,等.IL-33/ST2/ILC2s轴在哮喘发病中的作用及中医药干预研究进展[J].老年医学与保健,2019,25(6):860-864.
[9] 张文斌,王杰,王玮,等.健脾益肺汤抑制NF-κB/STAT3信号通路改善哮喘模型大鼠气道炎症及气道重塑作用研究[J].中国中医急症,2019,28(5):806-808,832.
[10] YANG Q,XU W,LONG Y,et al.STAT3 regulates cytokine expression in peripheral blood mononuclear cells from asthma patients[J].Cell Mol Biol(Noisy-le-grand),2017,63(9):71-74.
[11] 刘继红,冯斌.miR-98通过调控ox-LDL诱导血管内皮损伤抑制动脉粥样硬化形成的作用机制研究[J].中国循证心血管医学杂志,2018,11(8):970-974.
[12] 杨远舰,张桢,程哲,等.干扰STAT3基因对哮喘气道损伤的影响[J].中国病理生理杂志,2017,33(12):2269-2273.
[13] VALE K.Targeting the JAK-STAT pathway in the treatment of ‘Th2-high’ severe asthma[J].Future Med Chem,2016,8(4):405-419.
[14] LIU X,WEN Y,WANG D,et al.Synergistic activation of Src,ERK and STAT pathways in PBMCs for Staphylococcal enterotoxin A induced production of cytokines and chemokines[J].Asian Pac J Allergy Immunol,2020,38(1):52-63.
[15] YU Q N,GUO Y B,LI X,et al.ILC2 frequency and activity are inhibited by glucocorticoid treatment via STAT pathway in patients with asthma[J].Allergy,2018,73(9):1860-1870.

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