Objective: To explore the mechanism of propofol on regulating the growth of hepatocellular carcinoma(HCC) cells by mediating microRNA-637/signal transducer and activator of transcription 3(miR-637/STAT3). Methods: The human HCC cell line HepG2 was cultured normally and randomly divided into control group, low-dose, medium-dose and high-dose propofol(10, 25, 50 μg·ml-1) treatment groups. The proliferation, apoptosis, invasion and migration ability of the cells were respectively detected by CCK-8, flow cytometry, Transwell assay and scratch assay. The expressions of cleaved cysteinyl aspartate specific protease 3(cleaved caspase-3), B-cell lymphoma-2(Bcl-2), Bcl-associated X(Bax), matrix metalloproteinases(MMP)-2, MMP-9, STAT3 and phosphorylated STAT3(p-STAT3) proteins in cells were detected by Western blot. The expression level of miR-637 was determined by real-time quantitative polymerase chain reaction(qRT-PCR). Results: Compared with the control group, the rate of proliferation inhibition and cell apoptosis, expressions of cleaved caspase-3 and miR-637 were increased, while the number of invasion cells, migration rate, expressions of Bcl-2/Bax, MMP-2, MMP-9 and p-STAT3/STAT3 were decreased in the propofol treatment groups(P<0.05) in a dose-dependent manner. Conclusion: Propofol can inhibit the proliferation, invasion and migration of HCC cells, and induce their apoptosis by mediating miR-637/STAT3 axis. |
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