ATP6V1A在食管癌及食管上皮内瘤变组织中的表达及其临床意义-东南大学学报医学版

ATP6V1A在食管癌及食管上皮内瘤变组织中的表达及其临床意义

单位：1. 南通大学附属江阴医院消化内科, 江苏江阴 214400;
2. 徐州医科大学江阴临床学院消化内科, 江苏江阴 214400;
3. 南通大学附属江阴医院中心实验室, 江苏江阴 214400

分类号：R735.1;R571

出版年·卷·期（页码）：2021·40·第三期（366-370）

摘要：

目的：探讨空泡型质子泵的A亚基（ATP6V1A）在食管慢性炎、低级别上皮内瘤变、高级别上皮内瘤变、鳞癌组织中的表达情况及与食管癌进展的关系。方法：收集病理学诊断为慢性炎、低级别上皮内瘤变、高级别上皮内瘤变、鳞状细胞癌的食管病理蜡块标本，使用免疫组化法测定ATP6V1A在各组中的表达情况，分析其与性别、年龄、肿瘤部位、肿瘤直径、临床分期、淋巴结转移等临床参数之间的关系。结果：ATP6V1A在食管慢性炎、低级别上皮内瘤变、高级别上皮内瘤变、鳞癌组织中表达逐渐升高，差异有统计学意义（P<0.05）。ATP6V1A的表达与食管鳞癌患者临床分期、是否淋巴结转移显著相关。结论：ATP6V1A在食管癌的发展中可能起重要作用，检测其表达有助于食管癌的早期诊断及治疗方案的选择。

Objective: To investigate the expression of subunit A (ATP6V1A) of V-type proton ATPase in esophageal chronic inflammation, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia and squamous carcinoma tissues of esophagus, and its relationship with the progression of esophageal carcinoma. Methods: Pathological wax specimens of the esophagus pathologically diagnosed as chronic inflammation, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, and squamous carcinoma were collected for determining the expression of ATP6V1A in groups using immunohistochemical method, and analyzing its relationship with gender, age, tumor location, tumor size, clinical stage, lymph node metastasis and etc. Results: The expression levels of ATP6V1A in chronic inflammation, low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia, and squamous carcinoma tissues of esophagus were gradually increased, and the differences were statistically significant (P<0.05). The expression of ATP6V1A was significantly correlated with the clinical stage and lymph node metastasis for the patients with esophageal squamous carcinoma. Conclusion: ATP6V1A may play an important role in the development of esophageal carcinoma, and detecting its expression is conductive to the early diagnosis of esophageal carcinoma, and selection of relevant treatment regimens as well.

参考文献：

[1] 左婷婷, 郑荣寿, 曾红梅, 等. 中国食管癌发病状况与趋势分析[J]. 中华肿瘤杂志, 2016, 38(9):703-708.
[2] COTTER K, STRANSKY L, MCGUIRE C, et al. Recent insights into the structure, regulation, and function of the V-ATPases[J]. Trends Biochem Sci, 2015, 40(10):611-622.
[3] XU J, XIE R, LIU X, et al. Expression and functional role of vacuolar H(+)-ATPase in human hepatocellular carcinoma[J]. Carcinogenesis, 2012, 33(12):2432-2440.
[4] HINTON A, BOND S, FORGAC M.V-ATPase functions in normal and disease processes[J]. Pflug Arch Eur J Phy, 2008, 457(3):589-598.
[5] PÉREZ-SAYÁNS M, SOMOZA-MARTÍN M, BARROS-ANGUEIRA F, et al. Multidrug resistance in oral squamous cell carcinoma:the role of vacuolar ATPases[J]. Cancer Lett, 2010, 295(2):135-143.
[6] BRAY F, FERLAY J, SOERJOMATARAM I, et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J]. CA-Cancer J Clin, 2018, 68(6):394-424.
[7] JEFFERIES K C, CIPRIANO D J, FORGAC M.Function, structure and regulation of the vacuolar (H+)-ATPases[J]. Arch Biochem Biophys, 2008, 476(1):33-42.
[8] MCCONNELL M, FENG S, CHEN W, et al. Osteoclast proton pump regulator Atp6v1c1 enhances breast cancer growth by activating the mTORC1 pathway and bone metastasis by increasing V-ATPase activity[J]. Oncotarget, 2017, 8(29):47675-47690.
[9] WANG J, CHEN D, SONG W, et al. ATP6L promotes metastasis of colorectal cancer by inducing epithelial-mesenchymal transition[J]. Cancer Sci, 2020, 111(2):477-488.
[10] LU X, QIN W, LI J, et al. The growth and metastasis of human hepatocellular carcinoma xenografts are inhibited by small interfering RNA targeting to the subunit ATP6L of proton pump[J]. Cancer Res, 2005, 65(15):6843-6849.
[11] BARTEL K, WINZI M, ULRICH M, et al. V-ATPase inhibition increases cancer cell stiffness and blocks membrane related Ras signaling-a new option for HCC therapy[J]. Oncotarget, 2016, 8(6):9476-9487.
[12] OTERO-REY E M, SOMOZA-MARTÍN M, BARROS-ANGUEIRA F, et al. Intracellular pH regulation in oral squamous cell carcinoma is mediated by increased V-ATPase activity via over-expression of the ATP6V1C1 gene[J]. Oral Oncol, 2008, 44(2):193-199.
[13] LIU P, CHEN H, HAN L, et al. Expression and role of V1A subunit of V-ATPases in gastric cancer cells[J]. Int J Clin Oncol, 2015, 20(4):725-735.
[14] MARSHANSKY V, FUTAI M, GRVBER G.Eukaryotic V-ATPase and its super-complexes: from structure and function to disease and drug targeting, regulation of Ca²⁺-ATPases, V-ATPases and F-ATPases[M]. Cham, Switzerland: Springer International Publishing, 2016:301-335.

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