lncRNA LINC00339靶向miR-520a-3p对卵巢癌细胞增殖和侵袭的影响-东南大学学报医学版

lncRNA LINC00339靶向miR-520a-3p对卵巢癌细胞增殖和侵袭的影响

单位：沧州市中心医院妇产科, 河北沧州 061000

分类号：R737.9;R329.28

出版年·卷·期（页码）：2021·40·第二期（195-201）

摘要：

目的：探讨长链非编码RNA（lncRNA）LINC00339靶向调控miR-520a-3p对卵巢癌细胞增殖和侵袭的影响。方法：用逆转录-聚合酶链反应（RT-PCR）法检测卵巢癌细胞系（SKOV3、A2780、OVCAR3、HO-8910）和正常卵巢上皮细胞系（HOSEpiC）中LINC00339和miR-520a-3p的表达。分别将sh-NC（sh-NC组）和sh-LINC00339（sh-LINC00339组）转染入SKOV3细胞，克隆形成实验和MTT法检测细胞增殖，Transwell实验检测细胞侵袭。用双荧光素酶报告基因实验验证LINC00339与miR-520a-3p的靶向调控关系。为了进一步验证二者调控关系，将SKOV3细胞分为sh-NC+miR-NC组、sh-LINC00339+miR-NC组、sh-LINC00339+anti-miR-520a-3p组，比较3组细胞增殖和侵袭能力。结果：与HOSEpiC细胞比较，SKOV3、A2780、OVCAR3和HO-8910细胞中LINC00339相对表达量明显升高（均P<0.05），而miR-520a-3p相对表达量明显降低（均P<0.05）。与sh-NC组比较，sh-LINC00339组LINC00339相对表达量，克隆细胞数，转染24、48及72 h时细胞增殖活力、侵袭细胞数明显降低（均P<0.05）。双荧光素酶报告基因实验证实，LINC00339可以靶向作用于miR-520a-3p。与sh-NC+miR-NC组比较，sh-LINC00339+miR-NC组细胞增殖和侵袭力明显降低（均P<0.05）；与sh-LINC00339+miR-NC组比较，sh-LINC00339+anti-miR-520a-3p组细胞增殖和侵袭力明显升高（均P<0.05）。结论：LINC00339可能通过靶向调控miR-520a-3p促进卵巢癌细胞的增殖和侵袭。

Objective: To explore the effects of long-chain non-coding RNA(lncRNA) LINC00339 targeting miR-520a-3p on the proliferation and invasion of ovarian cancer cell. Methods: RT-PCR was used to detect the expression of LINC00339 and miR-520a-3p in ovarian cancer cell lines (SKOV3, A2780, OVCAR3, HO-8910) and normal ovarian epithelial cell lines (HOSEpiC). SKOV3 cells were transfected into sh-NC (sh-NC group) and sh-LINC00339 (LINC00339 group) respectively. Clone formation test and MTT method were used to detect cell proliferation; Transwell test was used to detect cell invasion. The dual luciferase reporter gene experiment was used to verify the targeted regulation relationship between LINC00339 and miR-520a-3p. In order to further verify the regulatory relationship between them, SKOV3 cells were divided into sh-NC+miR-NC group, sh-LINC00339+miR-NC group, sh-LINC00339+anti-miR-520a-3p group, and the cell proliferation and invasion of the three groups were compared. Results: Compared with HOSEpiC cells, the relative expression of LINC00339 in SKOV3, A2780, OVCAR3 and HO-8910 cells were significantly increased(all P<0.05), while the relative expression of miR-520a-3p was decreased (all P<0.05). Compared with the sh-NC group, the relative expression of LINC00339, the number of cloned cells, the cell proliferation activity and the number of invaded cells at 24, 48 and 72 h in the sh-LINC00339 group were decreased (P<0.05). The dual luciferase reporter gene experiment confirmed that LINC00339 can target miR-520a-3p. Compared with the sh-NC+miR-NC group, the sh-LINC00339+miR-NC group had lower cell proliferation and invasiveness (all P<0.05). Compared with the sh-LINC00339+miR-NC group, the sh-LINC00339+anti-miR-520a-3p group had higher cell proliferation and invasiveness (P<0.05). Conclusion: LINC00339 may promote the proliferation and invasion of ovarian cancer cells through targeting regulation of miR-520a-3p.

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