沉默JMJD2B通过诱导DNA损伤抑制人胃癌细胞的恶性表型-东南大学学报医学版

沉默JMJD2B通过诱导DNA损伤抑制人胃癌细胞的恶性表型

单位：中山大学附属惠州市中心人民医院消化内科, 广东惠州 516000

分类号：R735.2

出版年·卷·期（页码）：2021·40·第一期（69-74）

摘要：

目的：探讨人胃癌细胞中含Jumonji结构域的蛋白2B （JMJD2B）与DNA损伤反应之间的关系，及其在肿瘤恶性表型中的作用。方法：应用JMJD2B siRNA特异性抑制人胃癌细胞MGC803和SGC7901中JMJD2B的表达，采用Western blotting检测经处理后DNA损伤特异性标志物磷酸化H2AX （γH2AX）的表达，通过DNA损伤检测方法彗星实验测定DNA拖尾的情况，并分别采用CCK-8和流式细胞仪分析细胞增殖、细胞周期分布及细胞凋亡情况。结果：人胃癌细胞MGC803和SGC7901中，JMJD2B siRNA特异性抑制了JMJD2B的表达，γH2AX表达上调，同时彗星实验提示DNA拖尾百分比明显增加，肿瘤细胞发生了G₂/M或G₀/G₁期阻滞，细胞凋亡比例增加，细胞增殖显著受抑（P<0.05）。结论：沉默JMJD2B可诱导人胃癌细胞发生DNA损伤，进而抑制了癌细胞的恶性表型。

Objective: To explore the relationship between JMJD2B and DNA damage response in human gastric cancer cells, and their role in tumor malignant phenotype. Methods: JMJD2B siRNAs were transiently transfected into human gastric cancer cells MGC803 and SGC7901 for silencing JMJD2B expression. The expression of DNA damage specific marker-phosphorylation expression of H2AX(γH2AX) was assessed by Western blotting, and Comet assay was used to investigate DNA damage. Cell proliferation was detected using CCK-8 assay, while flow cytometry was performed to detect cell cycle distribution and apoptosis. Results: JMJD2B siRNA specifically inhibited the expression of JMJD2B in human gastric cancer cells, which up-regulated the γH2AX, at the same time, Comet assay revealed the percentage of DNA in tail increased sharply thus led to cell cycle arrest in G₂/M or G₀/G₁ phase, increased proportion of cell apoptosis and significantly inhibited cell proliferation (P<0.05). Conclusion: Silencing JMJD2B induces DNA damage response in human gastric cancer cells, which in turn inhibits the malignant phenotype of cancer cells.

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