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沉默JMJD2B通过诱导DNA损伤抑制人胃癌细胞的恶性表型
作者:陈丽莎  徐永成  曾书君  陈惠新 
单位:中山大学附属惠州市中心人民医院 消化内科, 广东 惠州 516000
关键词:组蛋白去甲基化酶JMJD2B DNA损伤反应 人胃癌细胞 
分类号:R735.2
出版年·卷·期(页码):2021·40·第一期(69-74)
摘要:

目的:探讨人胃癌细胞中含Jumonji结构域的蛋白2B (JMJD2B)与DNA损伤反应之间的关系,及其在肿瘤恶性表型中的作用。方法:应用JMJD2B siRNA特异性抑制人胃癌细胞MGC803和SGC7901中JMJD2B的表达,采用Western blotting检测经处理后DNA损伤特异性标志物磷酸化H2AX (γH2AX)的表达,通过DNA损伤检测方法彗星实验测定DNA拖尾的情况,并分别采用CCK-8和流式细胞仪分析细胞增殖、细胞周期分布及细胞凋亡情况。结果:人胃癌细胞MGC803和SGC7901中,JMJD2B siRNA特异性抑制了JMJD2B的表达,γH2AX表达上调,同时彗星实验提示DNA拖尾百分比明显增加,肿瘤细胞发生了G2/M或G0/G1期阻滞,细胞凋亡比例增加,细胞增殖显著受抑(P<0.05)。结论:沉默JMJD2B可诱导人胃癌细胞发生DNA损伤,进而抑制了癌细胞的恶性表型。

Objective: To explore the relationship between JMJD2B and DNA damage response in human gastric cancer cells, and their role in tumor malignant phenotype. Methods: JMJD2B siRNAs were transiently transfected into human gastric cancer cells MGC803 and SGC7901 for silencing JMJD2B expression. The expression of DNA damage specific marker-phosphorylation expression of H2AX(γH2AX) was assessed by Western blotting, and Comet assay was used to investigate DNA damage. Cell proliferation was detected using CCK-8 assay, while flow cytometry was performed to detect cell cycle distribution and apoptosis. Results: JMJD2B siRNA specifically inhibited the expression of JMJD2B in human gastric cancer cells, which up-regulated the γH2AX, at the same time, Comet assay revealed the percentage of DNA in tail increased sharply thus led to cell cycle arrest in G2/M or G0/G1 phase, increased proportion of cell apoptosis and significantly inhibited cell proliferation (P<0.05). Conclusion: Silencing JMJD2B induces DNA damage response in human gastric cancer cells, which in turn inhibits the malignant phenotype of cancer cells.

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