目的：探讨基质金属蛋白酶14（MMP14）在肝细胞癌（HCC）组织中的表达及其与患者预后的关系。方法：收集2008年6月至2013年5月本院手术切除的248例HCC癌组织标本及其配对的癌旁组织标本（距离癌组织边缘≥5 cm）。随机选取6例癌组织及其配对的癌旁组织，用Western blotting检测组织中MMP14蛋白相对表达量。采用免疫组织化学染色法检测所有组织中MMP14的表达水平，根据染色结果将HCC患者分为高表达组和低表达组。比较两组患者的临床病理特征和生存预后。结果：HCC癌组织中MMP14蛋白相对表达量为0.91±0.08，明显高于癌旁组织的0.24±0.08（P<0.05）。癌组织中MMP14高表达率为62.10%，明显高于癌旁组织的26.61%（P<0.001）。MMP14与HCC分化程度及TNM分期有关（P<0.05）。肿瘤低分化、高TNM分期和MMP14高表达是HCC患者总生存期和无瘤生存期的独立影响因素（P<0.05）。MMP14高表达患者的总体生存时间和无瘤生存时间明显低于低表达患者（P<0.05）。结论：HCC癌组织中MMP14表达水平明显高于癌旁组织。MMP14是HCC预后的独立影响因素，其表达水平越高，患者总生存期和无瘤生存期越短。

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