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血清中异常表达的微小RNA-324-5p在动脉粥样硬化诊断和预后评估中的价值分析
作者:刘海峰1  刘红伟1  马宝新2 
单位:1. 山东省广饶县人民医院 心血管内科, 山东 东营 257300;
2. 滨州医学院附属医院 心血管内科, 山东 滨州 256600
关键词:动脉粥样硬化 微小RNA-324-5p 诊断 预后 
分类号:R543.12
出版年·卷·期(页码):2020·39·第一期(48-52)
摘要:

目的:通过检测微小RNA-324-5p(miR-324-5p)在动脉粥样硬化(AS)患者血清中的表达水平,分析miR-324-5p在临床中的诊断和预后评估价值。方法:通过实时荧光定量PCR技术检测AS患者与健康对照人群血清中miR-324-5p的表达水平;采用Spearman相关系数分析miR-324-5p的表达与颈动脉内膜-中膜厚度(CIMT)的相关性;通过受试者工作特征曲线(ROC曲线)评估miR-324-5p在AS中的诊断价值;通过Kaplan-Meier生存曲线、单因素和多因素Cox回归分析检测miR-324-5p在AS中的预后评估价值。结果:AS患者血清中miR-324-5p的表达水平较健康对照人群显著下降(P<0.001),且miR-324-5p在CIMT值较低的患者中表达,两者为负相关性(r=-0.8043,P<0.001)。ROC曲线下面积为0.874,具有较高的敏感性(80%)和特异性(87.1%),证明miR-324-5p在AS患者中具有较高的诊断准确性。同时发现miR-324-5p可以作为AS的独立预后因子(HR=3.318,95% CI=1.219~9.031,P=0.019),低表达miR-324-5p患者发生心血管事件的概率较高(log-rank P=0.002)。结论:低表达miR-324-5p可以作为AS诊断的一种潜在标志物,并且与AS的不良预后相关。

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