目的：探究微小RNR-133（miR-133）对肝癌细胞增殖、侵袭和迁移的影响及其作用机制。方法：采用实时定量PCR检测miR-133在肝癌组织或肝癌细胞中的表达。采用Lipofectamine 2000试剂转染miR-133模拟物和miR-133抑制物，随后采用CCK-8和Transwell实验分析人肝癌细胞系HepG2细胞的增殖、侵袭和迁移能力变化。通过PicTar软件预测miR-133的靶基因，并进一步通过双荧光素酶报告实验验证其结合能力。最后利用蛋白质印迹法检测蛋白表达变化。结果：miR-133在肝癌组织和肝癌细胞中的表达显著降低。miR-133能够抑制HepG2细胞的增殖、迁移及侵袭能力。此外，miR-133能够靶向调控表皮生长因子受体（EGFR）的表达，敲低EGFR防止了miR-133下调对肿瘤细胞增殖、侵袭及迁移能力的促进作用。结论：miR-133通过影响EGFR表达而抑制肝癌细胞的增殖、侵袭及迁移。

[1] EL-SERAG H B.Hepatocellular carcinoma[J].N Engl J Med,2011,365(12):1118-1127.
[2] 蔡林,廖伯年.白花蛇舌草解毒健脾方对原发性肝癌晚期患者肿瘤相关指标及疼痛的影响[J].东南大学学报(医学版),2017,36(1):44-47.
[3] CHOI S S,DIEHL A M.Epithelial-to-mesenchymal transitions in the liver[J].Hepatology,2009,50(6):2007-2013.
[4] VAN ZIJL F,ZULEHNER G,PETZ M,et al.Epithelial-mesenchymal transition in hepatocellular carcinoma[J].Future Oncol,2009,5(8):1169-1179.
[5] FURUTA M,KOZAKI K,TANIMOTO K,et al.The tumor-suppressive miR-497-195 cluster targets multiple cell-cycle regulators in hepatocellular carcinoma[J].PLoS One,2013,8(3):e60155.
[6] HUMMEL R,HUSSEY D J,HAIER J.MicroRNAs:predictors and modifiers of chemo-and radiotherapy in different tumour types[J].Eur J Cancer,2010,46(2):298-311.
[7] DONG Z,ZHONG Z,YANG L,et al.MicroRNA-31 inhibits cisplatin-induced apoptosis in non-small cell lung cancer cells by regulating the drug transporter ABCB9[J].Cancer Lett,2014,343(2):249-257.
[8] NAMLOS H M,MEZA-ZEPEDA L A,BAROY T,et al.Modulation of the osteosarcoma expression phenotype by microRNAs[J].PLoS One,2012,7(10):e48086.
[9] ZHAO G,CAI C,YANG T,et al.MicroRNA-221 induces cell survival and cisplatin resistance through PI3K/Akt pathway in human osteosarcoma[J].PLoS One,2013,8(1):e53906.
[10] TRUINI A,COCO S,ALAMA A,et al.Role of microRNAs in malignant mesothelioma[J].Cell Mol Life Sci,2014,71(15):2865-2878.
[11] ZHAO-QI H,WEI X,JIN-LEI W,et al.MicroRNA-374a protects against myocardial ischemia-reperfusion injury in mice by targeting the MAPK6 pathway[J].Life Sci,2019,232:116619.
[12] YONGCHUN Z,LINWEI T,XICAI W,et al.MicroRNA-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB[J].Cancer Lett,2014,347(1):65-74.
[13] CHENG J,DENG H,XIAO B,et al.piR-823,a novel non-coding small RNA,demonstrates in vitro and in vivo tumor suppressive activity in human gastric cancer cells[J].Cancer Lett,2012,315(1):12-17.
[14] LIU G,JIANG C,LI D,et al.MiRNA-34a inhibits EGFR-signaling-dependent MMP7 activation in gastric cancer[J].Tumour Biol,2014,35(10):9801-9806.
[15] XIE Z,CAI L,LI R,et al. Down-regulation of miR-489 contributes into NSCLC cell invasion through targeting SUZ12[J].Tumour Biol,2015,36(8):6497-6505.
[16] 郭飞宇,杨军,熊书名,等.表皮生长因子受体和胸苷酸合成酶在原发性肝癌中的表达和意义[J].中华肝脏病杂志,2018,26(9):666-669.