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肝X受体激动后对人肝癌细胞SMMC-7721 Warburg效应和细胞侵袭力的影响
作者:郑翔宇  付宗强  李永伟 
单位:河南省中医院 检验科, 河南 郑州 450002
关键词:肝X受体 Warburg效应 侵袭 糖酵解 人肝癌细胞 
分类号:R36
出版年·卷·期(页码):2019·38·第六期(1038-1043)
摘要:

目的:研究肝X受体(LXRs)激动后对人肝癌细胞SMMC-7721 Warburg效应的调节作用及侵袭能力的影响。方法:体外培养人肝癌细胞SMMC-7721,分为对照组和实验组,实验组采用LXRs激动剂T0901317干预SMMC-7721细胞,对照组仅常规培养。分别在干预后12、24、48 h采用MTT法检测SMMC-7721细胞的活力,流式细胞术检测SMMC-7721细胞周期的改变,葡萄糖氧化酶法检测SMMC-7721细胞葡萄糖摄取水平,比色法检测细胞乳酸水平,Transwell小室法检测SMMC-7721细胞的侵袭能力,蛋白质印迹法检测己糖激酶(HKI)、磷酸果糖激酶(PFKP)、丙酮酸激酶(PK)、乳酸脱氢酶(LDH)、丙酮酸脱氢酶(PDH)水平。在干预后2、3、4周采用软琼脂集落形成实验检测克隆形成率评价细胞恶性转化。结果:MTT实验结果显示,SMMC-7721细胞活力实验组与对照组差异无统计学意义(P > 0.05)。SMMC-7721细胞周期实验组与对照组比较,差异无统计学意义(P > 0.05)。SMMC-7721细胞集落形成能力实验组显著低于对照组(P < 0.05)。SMMC-7721细胞葡萄糖的消耗水平和乳酸产生水平实验组均显著低于对照组(均P < 0.05)。SMMC-7721细胞的侵袭能力实验组著低于对照组(P < 0.05),SMMC-7721细胞中糖酵解关键酶HKI、PFKP、PK、LDH、PDH水平实验组均显著低于对照组(均P < 0.05)。结论:激动LXRs受体可以抑制肝癌细胞SMMC-7721的Warburg效应,抑制其侵袭。

Objective: To study the effect of hepatic X receptor (LXRs) after activation on the Warburg effect of human hepatoma cell SMMC-7721 and effect on invasion. Methods: Human hepatoma cell SMMC-7721 was cultured, and then the cells were divided into the control group and the experimental group. LXRs agonist T0901317 was used to intervene SMMC-7721 cells in the experimental group. The cells in the control group was routinely cultured. The SMMC-7721 cell viability was detected by MTT after intervention 12, 24 and 48 h respectively, and the cell cycle changes were detected by flow cytometry and cell glucose uptake was detected by glucose oxidase assay. Lactic acid level was detected by colorimetric assay and cell invasiveness was detected by Transwell chamber method. The levels of hexokinase (HKI), phosphoric acid fructose kinase (PFKP), pyruvate kinase (PK), lactate dehydrogenase (LDH) and pyruvate dehydrogenase (PDH) were detected by Western blotting. The formation rate of soft agar colony formation test was used to detect cell malignant transformation after intervention 2,3,4 week respectively. Results: MTT test showed that there was no significant difference in SMMC-7721 cells viability between the experimental group and the control group (P > 0.05). The cell cycle of the experimental group had no significant change compared to the control group (P > 0.05). The SMMC-7721 cells colony test showed that the colony formation ability of the experimental group was significantly lower than that of the control group (P < 0.05). The glucose consumption level and lactic acid production level of the experimental group were significantly lower than those of the control group (all P < 0.05). The invasive ability of SMMC-7721 cells in the experimental group was significantly lower than that in the control group (P < 0.05). The levels of glycolytic key enzymes in the experimental group were significantly lower than those of the control group (P < 0.05), such as HKI, PFKP, PK, LDH and PDH. Conclusion: Activated LXRs receptor can inhibit the Warburg effect and inhibit the invasion of hepatocellular carcinoma cells SMMC-7721.

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