Objective: To study the effect of hepatic X receptor (LXRs) after activation on the Warburg effect of human hepatoma cell SMMC-7721 and effect on invasion. Methods: Human hepatoma cell SMMC-7721 was cultured, and then the cells were divided into the control group and the experimental group. LXRs agonist T0901317 was used to intervene SMMC-7721 cells in the experimental group. The cells in the control group was routinely cultured. The SMMC-7721 cell viability was detected by MTT after intervention 12, 24 and 48 h respectively, and the cell cycle changes were detected by flow cytometry and cell glucose uptake was detected by glucose oxidase assay. Lactic acid level was detected by colorimetric assay and cell invasiveness was detected by Transwell chamber method. The levels of hexokinase (HKI), phosphoric acid fructose kinase (PFKP), pyruvate kinase (PK), lactate dehydrogenase (LDH) and pyruvate dehydrogenase (PDH) were detected by Western blotting. The formation rate of soft agar colony formation test was used to detect cell malignant transformation after intervention 2,3,4 week respectively. Results: MTT test showed that there was no significant difference in SMMC-7721 cells viability between the experimental group and the control group (P > 0.05). The cell cycle of the experimental group had no significant change compared to the control group (P > 0.05). The SMMC-7721 cells colony test showed that the colony formation ability of the experimental group was significantly lower than that of the control group (P < 0.05). The glucose consumption level and lactic acid production level of the experimental group were significantly lower than those of the control group (all P < 0.05). The invasive ability of SMMC-7721 cells in the experimental group was significantly lower than that in the control group (P < 0.05). The levels of glycolytic key enzymes in the experimental group were significantly lower than those of the control group (P < 0.05), such as HKI, PFKP, PK, LDH and PDH. Conclusion: Activated LXRs receptor can inhibit the Warburg effect and inhibit the invasion of hepatocellular carcinoma cells SMMC-7721. |
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