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麦冬多糖对糖尿病围绝经期大鼠血清SOD、GSH-Px、CAT、MDA水平的影响
作者:宋娜  苏东峰  刘晓燕  高宇  刘畅  山秀杰 
单位:承德医学院附属医院 内分泌科, 河北 承德 067000
关键词:2型糖尿病 围绝经期 麦冬多糖 氧化应激 雌激素 大鼠 
分类号:R562
出版年·卷·期(页码):2019·38·第六期(979-984)
摘要:

目的:观察麦冬多糖对2型糖尿病围绝经期模型大鼠血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和丙二醛(MDA)水平的影响,并探讨其作用机制。方法:大鼠予高糖、高脂饲料喂养8周后,采用链脲佐菌素诱导联合切除两侧卵巢的方法建立2型糖尿病围绝经期大鼠模型。选取50只建模成功的雌性大鼠随机分为模型组、阳性对照组(造模后第10天予二甲双胍0.25 g·kg-1)以及麦冬多糖低、中、高剂量组(造模后第10天分别予100、200、400 mg·kg-1麦冬多糖),每组10只。另取10只大鼠作为假手术组。比较治疗前后各组大鼠体质量、血糖水平、SOD、GSH-Px、CAT和MDA水平的差异。ELISA法检测各组大鼠血清雌二醇(E2)、促卵泡生成素(FSH)和促黄体生成素(LH)水平的差异。结果:与模型组比较,阳性对照组以及麦冬多糖低、中和高剂量组大鼠体质量明显增加,血糖水平明显降低(均P < 0.05),血清SOD、GSH-Px和CAT水平明显升高(均P < 0.05),血清MDA水平明显降低(P < 0.05),血清E2水平明显升高(P < 0.05),血清FSH和LH水平明显降低(均P < 0.05)。结论:麦冬多糖对2型糖尿病围绝经期有一定保护作用,其机制可能与提高抗氧化能力和调节血清雌激素水平有关。

Objective: To observe the effects of Ophiopogon japonicuson polysaccharide on serum superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT) and malondialdehyde (MDA) in diabetic peri-menopausal rats, and explore its mechanism of action. Methods: The type 2 diabetes premenopausal rats were established by combined removal of bilateral ovaries with streptozotocin induction after giving high sugar and high fat feed for 8 weeks. Fifty model rats were randomly divided into model group,positive control group(melbine 0.25 g·kg-1 was given 10 days after molding) and Ophiopogon japonicuson polysaccharide low, medium and high dose group (100, 200, 400 mg·kg-1 were given respectively 10 days after molding), 10 in each group. Another 10 rats were selected as the sham operation group. The levels of body weight, blood glucose, serum SOD, GSH-Px, CAT and MDA in each group were compared before and after treatment. Levels of serum estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in each group were detected by ELISA and compared each other. Results: Compared with the model group, the body weight of the rats in the positive control group and low,medium, high dose polysaccharide groups were increased and blood glucose levels were decreased significantly (all P < 0.05).The levels of serum SOD, GSH-Px and CAT were significantly increased (all P < 0.05), and serum MDA were significantly decreased (P < 0.05); serum E2 was significantly increased (P < 0.05), serum FSH and LH were significantly decreased (all P < 0.05). Conclusion: Ophiopogon japonicuson polysaccharide can protect type 2 diabetes perimenopenesis, and its mechanism may be related to improving antioxidant capacity and regulating estrogen levels.

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