Objective: To observe the effects of Ophiopogon japonicuson polysaccharide on serum superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), catalase(CAT) and malondialdehyde (MDA) in diabetic peri-menopausal rats, and explore its mechanism of action. Methods: The type 2 diabetes premenopausal rats were established by combined removal of bilateral ovaries with streptozotocin induction after giving high sugar and high fat feed for 8 weeks. Fifty model rats were randomly divided into model group,positive control group(melbine 0.25 g·kg-1 was given 10 days after molding) and Ophiopogon japonicuson polysaccharide low, medium and high dose group (100, 200, 400 mg·kg-1 were given respectively 10 days after molding), 10 in each group. Another 10 rats were selected as the sham operation group. The levels of body weight, blood glucose, serum SOD, GSH-Px, CAT and MDA in each group were compared before and after treatment. Levels of serum estradiol (E2), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in each group were detected by ELISA and compared each other. Results: Compared with the model group, the body weight of the rats in the positive control group and low,medium, high dose polysaccharide groups were increased and blood glucose levels were decreased significantly (all P < 0.05).The levels of serum SOD, GSH-Px and CAT were significantly increased (all P < 0.05), and serum MDA were significantly decreased (P < 0.05); serum E2 was significantly increased (P < 0.05), serum FSH and LH were significantly decreased (all P < 0.05). Conclusion: Ophiopogon japonicuson polysaccharide can protect type 2 diabetes perimenopenesis, and its mechanism may be related to improving antioxidant capacity and regulating estrogen levels.
 吴媛,何枝荣,林评樱.关于AMH预测卵巢早衰、围绝经期的研究[J].临床医学工程, 2018, 25(6):745-746.
 王靓,倪青.围绝经期患者糖尿病中医病机探析[J].北京中医药, 2017, 36(6):540-542.
 高伟聪,李丽,刘改欣,等.雌激素替代治疗对2型糖尿病围绝经期妇女血糖的影响[J].临床与病理杂志, 2017, 37(5):1019-1024.
 冯华,顾晓芳.中药内服外洗法用于肾阴虚型围绝经期综合征患者治疗的临床效果[J].现代医学, 2017,36(12):1779-1780.
 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社, 2015:155-156.
 FAN Y, MA X, MA L, et al. Antioxidative and immunological activities of Ophiopogon polysaccharide liposome from the root of Ophiopogon japonicus[J]. Carbohydr Polym, 2016, 135(5):110-120.
 CHEN X M, JIN J, TANG J, et al. Extraction, purification, characterization and hypoglycemic activity of a polysaccharide isolated from the root of ophiopogon japonicus[J]. Carbohydr Polym, 2011, 83(2):749-754.
 王慧,闫朝丽,苏秀兰,等.氯沙坦改善2型糖尿病大鼠胰岛素抵抗的机制研究[J].河北医药, 2018, 40(11):1605-1608.
 王睿,刘吉成,罗春娟,等.葛根素对围绝经期抑郁症模型小鼠的神经保护作用及机制研究[J].神经解剖学杂志, 2017, 33(2):190-196.
 ZHANG J, FAN, S, MAO, Y, et al. Cardiovascular protective effect of polysaccharide from Ophiopogon japonicus in diabetic rats[J]. Int J Biol Macromol, 2016, 82(10):505-513.
 钱江,梁金强,邱玉文,等.黄芪和麦冬复合多糖对TNBS诱导结肠炎大鼠的改善作用研究[J].食品工业科技, 2017, 38(6):354-356.
 张小平,孙润广,王小梅,等.化学修饰水提麦冬多糖WPOJ的抗肿瘤活性研究[J].食品与生物技术学报, 2014, 33(4):368-373.
 王君君,吕建新,陈晓明.麦冬多糖抗氧化应激及对炎症反应的保护作用[D].温州:温州医科大学, 2011.
 CHEN X, TANG J, XIE W, et al. Protective effect of the polysaccharide from Ophiopogon japonicus on streptozotocin-induced diabetic rats[J]. Carbohydr Polym, 2013, 94(1):378-385.
 TIWARI M K, JENA N R, MISHRA P C. Mechanisms of scavenging superoxide, hydroxy l, nitrogen dioxide and methoxy radicals by allicin:catalytic role of superoxide dismutase in scavenging superoxide radical[J]. J Chem Sci, 2018, 130(8):105-108.
 KASZNICKI J, SLIWINSKA A, KOSMALSKI M, et al. Genetic polymorphisms (Pro197Leu of Gpx1,+35A/C of SOD1,-262C/T of CAT), the level of antioxidant proteins (GPx1, SOD1, CAT) and the risk of distal symmetric polyneuropathy in Polish patients with type 2 diabetes mellitus[J]. Adv Med Sci, 2016, 61(1):123-129.
 BLAGOJEVIC D, BUZADZIC B, KORAC B, et al. Seasonal changes in the antioxidative defense in ground squirrels (Citelluscitellus):possible role of GSH-Px[J]. J Environ Pathol Toxicol Oncol, 1998, 17(3-4):241-250.
 段纬喆,赵湜,毛红. 2型糖尿病膝下动脉病变患者介入治疗前后缺血动脉内氧化应激标志物的变化及意义[J].中国动脉硬化杂志, 2018,26(2):176-180.
 JIANG X R, REN L, LI C R. Effect of electroacupuncture on hypothalamus pituitary ovary (HPO) axis in rats with perimenopausal depression[J]. Acupunct Res, 2017, 42(1):45-49.
 QIN X, XIAO Y, YE C, et al. Pituitary action of E2 in prepubertal grass carp:receptor specificity and signal transduction for luteinizing hormone and follicle-stimulating hormone regulation[J]. Front Endocrinol, 2018, 41(9):308-314.