荧光原位杂交技术检测SYT-SSX融合基因对滑膜肉瘤的诊断价值分析-东南大学学报医学版

荧光原位杂交技术检测SYT-SSX融合基因对滑膜肉瘤的诊断价值分析

单位：荆州市第二人民医院检验科, 湖北荆州 434000

分类号：R738.5;R730.43

出版年·卷·期（页码）：2019·38·第五期（869-873）

摘要：

目的：探讨荧光原位杂交技术（FISH）检测SYT-SSX融合基因对滑膜肉瘤的诊断价值。方法：选择2013年6月至2017年6月我院收治的96例经病理诊断为滑膜肉瘤患者为研究对象，所有患者经手术或活检取组织制作组织芯片，应用FISH技术检测SYT-SSX融合基因，免疫组化（IHC）检测常规抗体表达，对比两种检测方法的结果。结果：IHC结果显示，96例患者中Vimentin、CD99、AEl/AE3、EMA、CD34、Calponin、Ki-67的阳性表达率分别为100.00%、73.96%、90.63%、6.25%、29.17%、16.67%、60.42%。FISH检测结果显示，90例患者存在SYT基因易位，其中58例为考虑滑膜肉瘤，32例为可疑滑膜肉瘤，SYT-SSX阳性细胞百分率16.25%时诊断滑膜肉瘤的灵敏度、特异度、准确率分别为91.14%、100.00%、93.02%。FISH与IHC诊断滑膜肉瘤具有一致性（Kappa=0.43）。结论：FISH技术检测SYT-SSX融合基因灵敏度和特异度高，与IHC具有较高一致性，诊断滑膜肉瘤时可互相补充。

Objective: To investigate the diagnostic value of fluorescence in situ hybridization(FISH) for detecting SYT-SSX fusion gene in synovial sarcoma. Methods: 96 patients with synovial sarcoma diagnosed by pathology in our hospital from June 2013 to June 2017 were selected as the study subjects.Tissue biopsies were obtained from all patients by surgery or biopsy.SYT-SSX fusion gene was detected by FISH technique.The expression of routine antibody was detected by immunohistochemical (IHC) method.The results of the two methods were compared. Results: In the 96 patients, the positive rates of Vimentin, CD99, AEl/AE3, EMA, CD34, Calponin and Ki-67 were 100%, 73.96%, 90.63%, 6.25%, 29.17%, 16.67% and 60.42%, respectively. 90 patients were detected with translocation of SYT gene by FISH, including 58 cases of synovial sarcoma and 32 suspected synovial sarcoma.When the percentage of SYT-SSX positive cells was 16.25%, the sensitivity, specificity and accuracy of diagnosing synovial sarcoma were 91.14%, 100.00% and 93.02%, respectively.FISH and IHC were consistent in diagnosing synovial sarcoma (Kappa=0.43). Conclusion: FISH technology has high sensitivity and specificity to detect SYT-SSX fusion gene. FISH and IHC have high consistency and can complement each other in the diagnosis of synovial sarcoma.

参考文献：

[1] KEROUANTON A,JIMENEZ I,CELLIER C,et al.Synovial sarcoma in children and adolescents[J].J Pediatr Hematol Oneol,2014,36(4):257-262.
[2] FETCHER C D M,BRIDGE J A,HOGENDOOM P,et al.WHO classification of tumors of soft tissue and bone[M].4th Edition.Lyon:IARC Press,2013:213-215.
[3] KUBO T,SHIMOSE S,FAJIMORI J,et al.Prognostic value of-SSl8-SSX fusion type in synovial sarcoma:systematic review and meta-analysis[J].Springer plus,2015,4:375.
[4] AMARY M F,BERISHA F,BEMARDI FDEL C,et al.Detection of SSl8-SSX fusion transcripts in formalin-fixed paraffin-embedded neoplasms:analysis of conventional RTPCR,qRTPCR and dual color FISH ag diagnostic tools for synovial garconla[J].Mod Pathol,2007,20(4):482-496.
[5] TIAN C,DING P,YUAN Z,et al.A novel dual EGFR/HER2 inhibitor KU004 induces cell cycle arrest and apoptosis in HER2-overcxpressing cancer cells[J].Apootosis,2015,20(12):1599-1612.
[6] FURUKAWA T,YOSHIMURA A,SUMIZAWA T,et al.Angiogenic factor[J].Nature,1992,356(6371):668.
[7] OZKAN E E,MERTSOYLU H,OZARDALI H I.A case of renal synovial sarcoma treated with adjuvant if osfamide and doxorubicin[J].Intern Med,2011,50(15):1575-1580.
[8] 赵玲俊.肠系膜滑膜肉瘤伴肺转移1例报告并文献复习[J].中国现代医生,2015,53(17):136-137.
[9] 王小桐,倪皓,周晓军,等.荧光原位杂交在诊断软组织肿瘤中的应用[J].中华病理学杂志,2016,45(12):889-894.
[10] 岳文香,李瑞慧,谢宝松,等.前纵隔滑膜肉瘤1例并文献复习[J].解放军医学杂志,2014,39(12):987-990.
[11] 袁勤钊,吴月琴,许刚,等.SYT-SSX基因检测联合免疫组织化学技术对滑膜肉瘤的临床诊断价值[J].肿瘤研究与临床,2016,28(4):226-230.
[12] 夏琛,吴义启,王晖,等.腹腔单相纤维型滑膜肉瘤3例1临床病理观察[J].诊断病理学杂志,2015,22(1):41-44.
[13] 徐艳,沈小星,刘标,等.肺腺癌中PLK1的异常表达及其临床病理意义[J].东南大学学报(医学版),2017,36(5):804-810.
[14] 徐朱烽,靳激扬.8例外周性原始神经外胚层肿瘤的CT、MRI表现并文献复习[J].东南大学学报(医学版),2017,36(3):403-408.
[15] 吴国友,李碧丽,吴岩,等.免疫组织化学、荧光原位杂交和逆转录——多聚酶链反应在滑膜肉瘤诊断中的价值[J].中国组织化学与细胞化学杂志,2016,25(2):179-182.
[16] FRIEDRICHS N,KRIEGL L,POREMBA C,et al.Pitfalb in the detection of t(1l:22)ttiranslocaon by fluorescence in sire hybridization and RT-PCR:a single-blinded study[J].Diagn Mol Pathol,2006,15(2):83-89.

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