Objective: To investigate the level of MicroRNA-34a-5p in rat liver fibrosis and activated hepatic stellate cells,and to clarify its role in hepatic fibrosis. Methods: The rats model of hepatic fibrosis were established.The hepatic stellate cells were activated by transforming growth factor-β1(TGF-β1). Morphological changes of liver tissue were observed by hematoxylin-eosin (HE) staining.The levels of α-smooth muscle actin (α-SMA) and MicroRNA-34a-5p in rat liver tissue and activated hepatic stellate cells were determined by reverse transcription-polymerase chain reaction (RT-PCR).Liver fibrosis model rats were divided into blank control group, negative transfection control group(transfected with microRNA-34a-5p mimetic negative control) and MicroRNA-34a-5p group(transfected with microRNA-34a-5p simulated matter) according to random number method;The TGF-β1 activated HSC-T6 cells were divided into blank control group, negative transfection control group and MicroRNA-34a-5p group.The α-SMA, collagen I, silencing regulator 1(SIRT1), secreted glycoprotein-3α (Wnt-3α), secreted glycoprotein-5α (Wnt-5α) and β-catenin levels of rat liver tissue and hepatic stellate cells were determined by RT-PCR and Western blotting. Results: Compared with the control group, the levels of α-SMA in the liver fibrosis tissue and activated hepatic stellate cells were increased (P<0.05), and the levels of MicroRNA-34a-5p were decreased (P<0.05).Compared with the blank control group and negative transfection control group, the levels of MicroRNA-34a-5p in the liver tissue and activated hepatic stellate cells of the microRNA-34a-5p group were increased (P<0.05), the α-SMA, collagen I, SIRT1, Wnt-3α, Wnt-5α, β-catenin levels decreased (P<0.05).There was no significant difference in each indicators in the liver tissue and activated hepatic stellate cells between the blank control group and negative transfection control group (P>0.05). Conclusion: MicroRNA-34a-5p level is decreased in hepatic fibrosis and activated hepatic stellate cells. MicroRNA-34a-5p may inhibit Wnt/β-catenin signaling pathway by targeting SIRT1, thereby exerts anti-fibrosis effect. |
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