Objective: To investigate the association between susceptibility gene single nucleotide polymorphisms (SNP)and lung cancer, to establish the prediction models for lung cancer based on the epidemiology and susceptibility gene SNP.Methods: From January to October 2017, a case-control study was performed for 100 lung cancer patients diagnosed by pathology and 100 healthy control individuals who were selected from Center of Health Examination. Epidemiological data mainly included general characteristics (gender, age, the current place of residence), smoking status, previous pneumonia,previous malignant, asbestos exposure,family lung cancer, chronic obstructive pulmonary diseases (COPD), previous pulmonary tuberculosis,bronchial asthma. The genetic polymorphisms of CYP1A1 rs4646903, CYP2E1 rs3813867, CYP1B1 rs1056836, GSTP1 rs1695, ERCC2 rs13181, XRCC1 rs25487, TP53 rs1042522, NQO1 rs1800566, CCND1 rs603965, MTHFR rs1801133, EPHX1 rs1051740 were examined by real-time PCR. Logistic regression model was performed to identify risk factors for lung cancer and calculated odds ratios (OR)and 95% CI . The classification ability of the model was evaluated using the area under the receiver operating characteristic (ROC) curve. Results: Lung cancer risk factors included age, smoking status, asbestos exposure, family lung cancer. No relationship was found between gender, previous pneumonia, previous malignant, COPD, previous pulmonary tuberculosis, bronchial asthma and lung cancer. The individuals who carried with CYP1B1 rs1056836 and GSTP1 rs1695 mutant heterozygote and homozygote had a high risk of lung cancer(OR=3.8, 95% CI 1.89-7.63, P<0.05;OR=3.3, 95% CI 1.73-6.3, P<0.05). The area under the curve were 0.839 and 0.857 based on environmental factors and inherent factors without SNP and with SNP. Conclusion: Acccording to the multiple logistic regression, age, smoking status,asbestos exposure,family lung cancer, genetic polymorphisms of CYP1B1 rs1056836 and GSTP1 rs1695 were risk factors for lung cancer. The two prediction models by this thesis can be used to predict the risk of lung cancer preferably. |
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