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表皮生长因子受体4在胃癌组织中的表达及其对胃癌细胞增殖、侵袭和迁移的影响
作者:刘粉霞1  陈丽1  孙宁2  吴亚薇1 
单位:1. 河南省中医院 病理科, 河南 郑州 450002;
2. 河南中医药大学 基础医学院, 河南 郑州 450002
关键词:表皮生长因子受体4 胃癌 细胞 增殖 侵袭 迁移 
分类号:R735.2;Q279
出版年·卷·期(页码):2019·38·第五期(848-853)
摘要:

目的:探讨表皮生长因子受体4(ErbB4)在胃癌组织中的表达及其对胃癌细胞增殖、侵袭和迁移的影响。方法:收集河南省中医院2016年1月至2016年12月胃癌根治术患者胃癌组织标本30例及对应的癌旁组织标本30例,将胃癌细胞SGC-7901分为对照组(加入不含AG1478的培养基)、AG1478组1(加入AG1478浓度为10 μmol·L-1的培养基)、AG1478组2(加入AG1478浓度为20 μmol·L-1的培养基)、AG1478组3(加入AG1478浓度为50 μmol·L-1的培养基),采用免疫组化染色测定胃癌组织中ErbB4蛋白表达,采用蛋白质印迹法测定细胞中ErbB4蛋白水平,采用RT-PCR测定组织和细胞中ErbB4 mRNA水平,采用CCK8测定细胞增殖,采用Transwell实验测定细胞侵袭和迁移能力。结果:胃癌组织中ErbB4蛋白的阳性表达率高于癌旁组织(P<0.05),胃癌组织中ErbB4 mRNA水平高于癌旁组织(P<0.05)。SGC-7901细胞中ErbB4蛋白和ErbB4 mRNA水平高于GES-1细胞(P<0.05)。与对照组比较,AG1478组1、AG1478组2、AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力均降低(P<0.05);与AG1478组1比较,AG1478组2、AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力降低(P<0.05);与AG1478组2比较,AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力降低(P<0.05)。结论:ErbB4在胃癌组织中呈高表达,ErbB4可能通过促进细胞增殖和转移参与胃癌的发生发展。

Objective: To investigate the expression of epidermal growth factor receptor 4 (ErbB4) in gastric cancer and its effect on proliferation, invasion and migration of gastric cancer cells. Methods: 30 cases of gastric cancer tissue specimens and 30 cases of corresponding adjacent tissue specimens were collected from patients with radical gastrectomy in Henan Provincial Hospital of Traditional Chinese Medicine from January 2016 to December 2016.Gastric cancer cells SGC-7901 were divided into control group (added medium without AG1478), AG1478 group 1 (added medium with concentration of 10 μmol·L-1 AG1478), AG1478 group 2 (added medium with 20 μmol·L-1 AG1478), AG1478 group 3(added medium with 50 μmol·L-1AG1478).Immunohistochemical staining was used to determine ErbB4 protein expression in gastric cancer tissue.Western blotting was used to determine the level of ErbB4 protein in cells.ErbB4 mRNA levels in tissues and cells were determined by RT-PCR.The cell proliferation was measured by CCK8.The cell invasion and migration were measured by Transwell assay. Results: The positive expression rate of ErbB4 protein in gastric cancer tissues was higher than that in adjacent tissues (P<0.05).The level of ErbB4 mRNA in gastric cancer tissues was higher than that in adjacent tissues (P<0.05).The levels of ErbB4 protein and ErbB4 mRNA in SGC-7901 cells were higher than those in GES-1 cells (P<0.05).Compared with the control group, the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 1, AG1478 group 2 and AG1478 group 3 were decreased(P<0.05).Compared with AG1478 group 1,the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 2 and AG1478 group 3 cells were decreased(P<0.05).Compared with AG1478 group 2, the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 3 cells were decreased(P<0.05). Conclusion: ErbB4 is highly expressed in gastric cancer tissues, and ErbB4 may participate in the development of gastric cancer by promoting cell proliferation and metastasis.

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