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表皮生长因子受体4在胃癌组织中的表达及其对胃癌细胞增殖、侵袭和迁移的影响
作者:刘粉霞1  陈丽1  孙宁2  吴亚薇1 
单位:1. 河南省中医院 病理科, 河南 郑州 450002;
2. 河南中医药大学 基础医学院, 河南 郑州 450002
关键词:表皮生长因子受体4 胃癌 细胞 增殖 侵袭 迁移 
分类号:R735.2;Q279
出版年·卷·期(页码):2019·38·第五期(848-853)
摘要:

目的:探讨表皮生长因子受体4(ErbB4)在胃癌组织中的表达及其对胃癌细胞增殖、侵袭和迁移的影响。方法:收集河南省中医院2016年1月至2016年12月胃癌根治术患者胃癌组织标本30例及对应的癌旁组织标本30例,将胃癌细胞SGC-7901分为对照组(加入不含AG1478的培养基)、AG1478组1(加入AG1478浓度为10 μmol·L-1的培养基)、AG1478组2(加入AG1478浓度为20 μmol·L-1的培养基)、AG1478组3(加入AG1478浓度为50 μmol·L-1的培养基),采用免疫组化染色测定胃癌组织中ErbB4蛋白表达,采用蛋白质印迹法测定细胞中ErbB4蛋白水平,采用RT-PCR测定组织和细胞中ErbB4 mRNA水平,采用CCK8测定细胞增殖,采用Transwell实验测定细胞侵袭和迁移能力。结果:胃癌组织中ErbB4蛋白的阳性表达率高于癌旁组织(P<0.05),胃癌组织中ErbB4 mRNA水平高于癌旁组织(P<0.05)。SGC-7901细胞中ErbB4蛋白和ErbB4 mRNA水平高于GES-1细胞(P<0.05)。与对照组比较,AG1478组1、AG1478组2、AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力均降低(P<0.05);与AG1478组1比较,AG1478组2、AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力降低(P<0.05);与AG1478组2比较,AG1478组3细胞中ErbB4蛋白和mRNA水平、细胞吸光值、细胞侵袭和迁移能力降低(P<0.05)。结论:ErbB4在胃癌组织中呈高表达,ErbB4可能通过促进细胞增殖和转移参与胃癌的发生发展。

Objective: To investigate the expression of epidermal growth factor receptor 4 (ErbB4) in gastric cancer and its effect on proliferation, invasion and migration of gastric cancer cells. Methods: 30 cases of gastric cancer tissue specimens and 30 cases of corresponding adjacent tissue specimens were collected from patients with radical gastrectomy in Henan Provincial Hospital of Traditional Chinese Medicine from January 2016 to December 2016.Gastric cancer cells SGC-7901 were divided into control group (added medium without AG1478), AG1478 group 1 (added medium with concentration of 10 μmol·L-1 AG1478), AG1478 group 2 (added medium with 20 μmol·L-1 AG1478), AG1478 group 3(added medium with 50 μmol·L-1AG1478).Immunohistochemical staining was used to determine ErbB4 protein expression in gastric cancer tissue.Western blotting was used to determine the level of ErbB4 protein in cells.ErbB4 mRNA levels in tissues and cells were determined by RT-PCR.The cell proliferation was measured by CCK8.The cell invasion and migration were measured by Transwell assay. Results: The positive expression rate of ErbB4 protein in gastric cancer tissues was higher than that in adjacent tissues (P<0.05).The level of ErbB4 mRNA in gastric cancer tissues was higher than that in adjacent tissues (P<0.05).The levels of ErbB4 protein and ErbB4 mRNA in SGC-7901 cells were higher than those in GES-1 cells (P<0.05).Compared with the control group, the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 1, AG1478 group 2 and AG1478 group 3 were decreased(P<0.05).Compared with AG1478 group 1,the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 2 and AG1478 group 3 cells were decreased(P<0.05).Compared with AG1478 group 2, the ErbB4 protein and mRNA levels, cell absorbance, cell invasion and migration ability in AG1478 group 3 cells were decreased(P<0.05). Conclusion: ErbB4 is highly expressed in gastric cancer tissues, and ErbB4 may participate in the development of gastric cancer by promoting cell proliferation and metastasis.

参考文献:

[1] LAKS S,MEYERS M O,KIM H J.Surveillance for gastric cancer[J].Surg Clin North Am,2017,97(2):317-331.
[2] KATONA B W,RUSTGI A K.Gastric cancer genomics:advances and future dIrections[J].Cell Mol Gastroenterol Hepatol,2017,3(2):211-217.
[3] GÜLER TEZEL G,ŞENER E,AYDIN Ç,et al.Prevalence of epidermal growth factor receptor mutations in patients with non-small cell lung gancer in Turkish population[J].Balkan Med J,2017,34(6):567-571.
[4] SUN E G,LEE K H,KO Y S,et al.KITENIN functions as a fine regulator of ErbB4 expression level in colorectal cancer via protection of ErbB4 from E3-ligase Nrdp1-mediated degradation[J].Mol Carcinog,2017,56(3):1068-1081.
[5] CHO Y T,CHEN K L,SHEEN Y S,et al.Purpuric drug eruptions caused by epidermal growth factor receptor inhibitors for non-small cell lung cancer:a clinicopathologic study of 32 cases[J].JAMA Dermatol,2017,153(9):906-910.
[6] MENEZES S V,SAHNI S,KOVACEVIC Z,et al.Interplay of the iron-regulated metastasis suppressor NDRG1 with epidermal growth factor receptor (EGFR) and oncogenic signaling[J].J Biol Chem,2017,292(31):12772-12782.
[7] LIU Y,SONG L,NI H,et al.ERBB4 acts as a suppressor in the development of hepatocellular carcinoma[J].Carcinogenesis,2017,38(4):465-473.
[8] SAHU A,PATRA P K,YADAV M K,et al.Identification and characterization of ErbB4 kinase inhibitors for effective breast cancer therapy[J].J Recept Signal Transduct Res,2017,37(5):470-480.
[9] MA X,LI L,TIAN T,et al.Study of lung cancer regulatory network that involves erbB4 and tumor marker gene[J].Saudi J Biol Sci,2017,24(3):649-657.
[10] SAGLAM O,XIONG Y,MARCHION D C,et al.ERBB4 expression in ovarian serous carcinoma resistant to platinum-based therapy[J].Cancer Control,2017,24(1):89-95.
[11] OHASHI Y,KUMAGAI K,MIYATA Y,et al.Overexpression of ErbB4 is an independent marker for lymph node metastasis in Japanese patients with oral squamous cell carcinoma[J].Oral Surg Oral Med Oral Pathol Oral Radiol,2016,122(3):313-321.
[12] 崔曼莉,张超,张明鑫,等.HIF-1α与ERBB4在食管鳞癌中的表达及意义[J].山西医科大学学报,2016,47(9):830-833.
[13] 谭永辉,吕品,聂盛丹,等.肝内胆管癌中ErbB4的表达及临床意义[J].中国普通外科杂志,2015,24(2):180-184.
[14] ZHANG M,ZHANG L,CUI M,et al.miR-302b inhibits cancer-related inflammation by targeting ERBB4,IRF2 and CXCR4 in esophageal cancer[J].Oncotarget,2017,8(30):49053-49063.
[15] SONG G,ZHANG H,CHEN C,et al.miR-551b regulates epithelial-mesenchymal transition and metastasis of gastric cancer by inhibiting ERBB4 expression[J].Oncotarget,2017,8(28):45725-45735.
[16] 叶文广,姚青林,张明鑫,等.miR-520a调控ErbB4的表达并抑制食管鳞癌细胞的增殖与侵袭[J].南方医科大学学报,2014,34(2):164-168.
[17] 彭思远,曾杰宏,谢博文,等.阻断ErbB4受体对人胆管细胞癌QBC939细胞系增殖与转移的影响[J].湖南师范大学学报(医学版),2017,14(2):23-26.
[18] 杨锁萍,丁为民,李凯,等.EGFR突变阴性非小细胞肺癌二、三线治疗的荟萃分析[J].东南大学学报(医学版),2015,34(6):859-867.
[19] 郑亮,刘爽,亓倩,等.干扰Mig-6基因表达对非小细胞肺癌EGFR信号通路的影响[J].东南大学学报(医学版),2017,36(3):416-421.
[20] BOJKO A,CIERNIAK A,ADAMCZYK A,et al.Modulatory effects of curcumin and tyrphostins (AG494 and AG1478) on growth regulation and viability of LN229 human brain cancer cells[J].Nutr Cancer,2015,67(7):1170-1182.
[21] 马聪玲,殷一萍,毛婷婷,等.EGFR抑制剂AG1478对宫颈癌HeLa细胞凋亡的影响[J].实用医学杂志,2017,33(2):202-205.

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