Objective: To observe the effects of acupoint catgut-embedding on the therapeutic effect of obese rats and the expression of fat-related genes in adipose and liver tissue. Methods: 40 rats were divided into blank group (n=8) and high-fat group (n=32). The rats in the blank group were fed with normal diet for 16 weeks. The rats in the high-fat group were fed with high-fat diet for 12 weeks firstly, to establish a model of diet-induced obese rats, and then the successful molding obese model rats(n=16) were randomly divided into catgut-embedding group and model group, each group with eight rats. The differences in body weight, body fat, and blood lipids of the rats in each group were compared. Morphological differences of adipocytes of rats in each group were observed by HE staining. Serum leptin levels were measured by ELISA, q-PCR was used to detect the expressions of PPARα, PPARγ and UCP1 in adipose tissue and PPARα, PPARγ, CPT1 in liver tissue. Results: Compared with the model group,the body weight of the catgut-embedding group was significantly lower (P<0.01), fat content in the perirenal fat and epididymal fat was significantly reduced(P<0.01 or P<0.05), serum TC, TG and LDL-C were decreased significantly(all P<0.01), serum HDL-C was increased significantly(P<0.01), the serum leptin content decreased significantly(P<0.01). Compared with the model group,the expression of PPARα in adipose tissue and liver tissue was significantly increased in the catgut-embedding group(all P<0.01), and the expression of PPARγ in adipose tissue and liver tissue was significantly decreased(P<0.01 or P<0.05), the expression of CPT1 in liver tissue and the expression of UCP1 in adipose tissue were all significantly increased(all P<0.05). Conclusion: Acupoint catgut-embedding can effectively reduce body weight, reduce visceral fat, reduce blood lipid levels, reduce serum leptin content, down-regulate the expression of PPARγ in adipose and liver tissue, up-regulate the expression of PPARα, UCP1 in adipose tissue and the expression of PPARα, CPT1 in liver tissue of obese rats, so as to achieve the purpose of treating obesity.
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