艾司西酞普兰对强迫症大鼠血清GDNF、BDNF水平及脑组织GDNF、BDNF、5-HT含量的影响-东南大学学报医学版

艾司西酞普兰对强迫症大鼠血清GDNF、BDNF水平及脑组织GDNF、BDNF、5-HT含量的影响

单位：郑州大学第一附属医院精神医学科, 河南郑州 450052

关键词：艾司西酞普兰强迫症胶质细胞源性神经营养因子脑源性神经营养因子五羟色胺大鼠

分类号：R-33;R749.72

出版年·卷·期（页码）：2019·38·第四期（677-681）

摘要：

目的：探讨艾司西酞普兰对强迫症大鼠胶质细胞源性神经营养因子（glial cell-derived neurotrophic factor，GDNF）、脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）水平及脑组织五羟色胺（serotonin，5-HT）含量的影响。方法：将42只大鼠根据随机数字法分为对照组、模型组（建立强迫症模型）和艾司西酞普兰组（建立强迫症模型+艾司西酞普兰治疗），每组14只。采用大鼠旷场法测试大鼠行为，采用酶联免疫吸附实验（ELISA）法测定血清GDNF和BDNF水平，采用Western blotting法测定脑组织GDNF和BDNF含量，采用高效液相结合电化学法测定脑组织5-HT含量。结果：模型组和艾司西酞普兰组参观地点、旋转频率、不动持续时间均低于对照组（P<0.05），运动总距离、移动频率均高于对照组（P<0.05）；艾司西酞普兰组参观地点、旋转频率、不动持续时间均高于模型组（P<0.05），运动总距离、移动频率低于模型组（P<0.05）。3组中心区频率和中心区持续时间比较差异均无统计学意义（P>0.05）。血清、脑组织GDNF及BDNF水平模型组和艾司西酞普兰组均低于对照组（P<0.05），艾司西酞普兰组均高于模型组（P<0.05）。模型组和艾司西酞普兰组脑组织5-HT水平均低于对照组（P<0.05），艾司西酞普兰组脑组织5-HT水平高于模型组（P<0.05）。结论：艾司西酞普兰可升高强迫症大鼠血清GDNF、BDNF水平以及脑组织GDNF、BDNF、5-HT含量，从而发挥对强迫症的治疗作用。

Objective: To investigate the effect of escitalopram on the levels of glial cell-derived neurotrophic factor(GDNF), brain-derived neurotrophic factor(BDNF) and serotonin(5-HT) content in brain tissue of obsessive-compulsive disorder rats. Methods: Forty-two rats were divided into control group, model group(established obsessive-compulsive disorder model) and escitalopram group(established obsessive-compulsive disorder model + escitalopram treatment) according to the random table method, with 14 rats in each group. The rats' behaviors were tested by rat open field test. The serum GDNF and BDNF levels were measured by enzyme linked immunosorbent assay (ELISA). The levels of brain GDNF and BDNF protein were determined by Western blotting. The level of 5-HT in brain tissue was determined by HPLC using electrochemical method. Results: The site of visit, rotation frequency and immobility duration of the model group and the escitalopram group were lower than those of the control group (P<0.05), and the total distance and frequency of movement were higher than those of the control group (P<0.05).The site of visit, rotation frequency and immobility duration of the escitalopram group were higher than those of the model group (P<0.05), and the total distance and frequency of movement in the escitalopram group were lower than that in the model group (P<0.05).There were no significant differences in the frequency of the central area and the duration of the central area among the three groups (P>0.05).The levels of serum and brain tissue GDNF and BDNF in the model group and the escitalopram group were lower than those in the control group (P<0.05).The serum and brain tissue GDNF and BDNF levels in the escitalopram group were higher than that in the model group (P<0.05).The 5-HT levels in brain tissue of the model group and the escitalopram group were lower than those in the control group (P<0.05).The level of 5-HT in brain of the escitalopram group was higher than that of the model group (P<0.05).Conclusion: Escitalopram can increase serum and brain tissue GDNF and BDNF levels in obsessive-compulsive disorder rats, and increase the content of 5-HT in brain tissue to exert its therapeutic effect on obsessive-compulsive disorder.

参考文献：

[1] GOODMAN W K,GRICE D E,LAPIDUS K A,et al.Obsessive-compulsive disorder[J].Psychiatr Clin North Am,2014,37(3):257-267.
[2] PIZARRO M,FONTENELLE L F,PARAVIDINO D C,et al.An updated review of antidepressants with marked serotonergic effects in obsessive-compulsive disorder[J].Expert Opin Pharmacother,2014,15(10):1391-1401.
[3] HOU Y C,LAI C H.Rapid responses of high-dose combined therapy of escitalopram and aripiprazole in a case of severe obsessive compulsive disorder with delusion[J].J Neuropsychiatry Clin Neurosci,2014,26(2):E44-45.
[4] 赵琳琳.艾司西酞普兰与帕罗西汀治疗强迫症的疗效及不良反应差异分析[J].中国实用神经疾病杂志,2016,19(17):126-128.
[5] 陈茜,胡嘉波,周朝昀,等.难治性强迫症胶质细胞源性神经营养因子水平与认知功能[J].中国神经精神疾病杂志,2015,41(8):497-500.
[6] WANG X,MIN S,XIE F,et al.Glial cell-derived neurotrophic factor alleviates sepsis-induced neuromuscular dysfunction by decreasing the expression of γ-and α7-nicotinic acetylcholine receptors in an experimental rat model of neuromyopathy[J].Biochem Biophys Res Commun,2018,496(2):260-266.
[7] GUO Y,LUO C,TU G,et al.Rhynchophylline downregulates phosphorylated cAMP response element binding protein,nuclear receptor-related-1,and brain-derived neurotrophic factor expression in the hippocampus of ketamine-induced conditioned place preference rats[J].Pharmacogn Mag,2018,14(53):81-86.
[8] 王欣晨.汉族人群帕罗西汀血药浓度和BDNF、GDNF基因多态性与抗抑郁疗效的相关分析[D].合肥:安徽医科大学,2013.
[9] HO E V,THOMPSON S L,KATZKA W R,et al.Clinically effective OCD treatment prevents 5-HT1B receptor-induced repetitive behavior and striatal activation[J].Psychopharmacology (Berl),2016,233(1):57-70.
[10] MAK L,STREINER D L,STEINER M.Is serotonin transporter polymorphism (5-HTTLPR) allele status a predictor for obsessive-compulsive disorder? A meta-analysis[J].Arch Womens Ment Health,2015,18(3):435-445.
[11] 陈春暖,陈祥荣,蔡乾昆,等.GAPDH降解途径在鱼藤酮诱导多巴胺能神经元损伤中的作用[J].中国组织化学与细胞化学杂志,2018,27(4):321-326.
[12] RUVEN C,BADEA S R,WONG W M,et al.Combination treatment with exogenous GDNF and fetal spinal cord cells results in better motoneuron survival and functional recovery after avulsion injury with delayed root reimplantation[J].J Neuropathol Exp Neurol,2018,77(4):325-343.
[13] BAGHERI A,TALEI S,HASSANZADEH N,et al.The neuroprotective effects of flaxseed oil supplementation on functional motor recovery in a model of ischemic brain stroke:upregulation of BDNF and GDNF[J].Acta Med Iran,2017,55(12):785-792.
[14] 王申捷,孙凡,朱亮.选择性5-羟色胺再摄取抑制剂的临床应用进展[J].现代生物医学进展,2014,14(3):571-574.
[15] MORI A,NISHIOKA Y,YAMADA M,et al.Brain-derived neurotrophic factor induces angiogenin secretion and nuclear translocation in human umbilical vein endothelial cells[J].Pathol Res Pract,2018,214(4):521-526.
[16] MARIE C,PEDARD M,QUIRIÉ A,et al.Brain-derived neurotrophic factor secreted by the cerebral endothelium:a new actor of brainfunction?[J].J Cereb Blood Flow Metab,2018,38(6):935-949.
[17] STRYJER R,DAMBINSKY Y,TIMINSKY I,et al.Escitalopram in the treatment of patients with schizophrenia and obsessive-compulsive disorder:an open-label,prospective study[J].Int Clin Psychopharmacol,2013,28(2):96-98.
[18] BERGMAN J,PASHINIAN A,WEIZMAN A,et al.The beneficial effect of escitalopram on obsessive-compulsive-related musical hallucinations in elderly patients with hearing impairment:a case series[J].Int Clin Psychopharmacol,2014,29(5):263-265.
[19] LOCHNER C,SIMMONS C,KIDD M,et al.Differential effects of escitalopram challenge on disgust processing in obsessive-compulsive disorder[J].Behav Brain Res,2012,226(1):274-280.
[20] 晏鑫鹏,饶莹.艾司西酞普兰与帕罗西汀治疗强迫症的疗效比较[J].上海医药,2017,38(18):22-24.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录