复方丹参对糖尿病大鼠种植体骨结合及TGF-β1表达的影响-东南大学学报医学版

复方丹参对糖尿病大鼠种植体骨结合及TGF-β1表达的影响

单位：1. 南方医科大学口腔医院(广东省口腔医院)番禺院区种植修复科, 广东广州 510260;
2. 成都学院(成都大学)医学院口腔教研组, 四川成都 610106;
3. 核工业四一六医院口腔科, 四川成都 610051

分类号：R-33;R587.1

出版年·卷·期（页码）：2019·38·第四期（651-656）

摘要：

目的：探讨复方丹参对糖尿病大鼠种植体骨结合及转化生长因子β1（TGF-β1）表达的影响。方法：将45只大鼠按随机数字表法均分为对照组、模型组（建立糖尿病模型）、复方丹参组（建立糖尿病模型+复方丹参片灌胃），测定治疗前后血糖和胰岛素水平，CT扫描种植体周围股骨标本的骨小梁数目、骨小梁厚度、骨小梁间隙、骨体积分数，免疫组化法测定种植体周围TGF-β1蛋白表达，采用RT-PCR法测定种植体周围TGF-β1 mRNA水平。结果：治疗前，与对照组比较，模型组和复方丹参组大鼠血糖水平明显升高（P<0.05），而胰岛素水平明显降低（P<0.05）；治疗后，与模型组比较，复方丹参组血糖和胰岛素均降低（均P<0.05）。治疗后，模型组和复方丹参组大鼠骨小梁数目、骨小梁厚度、骨体积分数、种植体最大拔出力、种植体周围TGF-β1水平均低于对照组（均P<0.05），骨小梁间隙大于对照组（P<0.05）；复方丹参组骨小梁数目、骨小梁厚度、骨体积分数、种植体最大拔出力、种植体周围TGF-β1水平高于模型组（均P<0.05），骨小梁间隙小于模型组（P<0.05）。结论：复方丹参可增加糖尿病大鼠种植体周围TGF-β1表达，促进种植体骨结合。

Objective: To investigate the effect of compound Danshen on implanted bone binding and expression of transforming growth factor-β1(TGF-β1) in diabetic rats. Methods: 45 rats were randomly divided into control group, model group(established diabetes model), and compound danshen group(established diabetic model plus compound danshen tablet). The level of blood glucose and insulin before and after treatment were determined. The number of trabecular bone, trabecular thickness, trabecular space and bone volume fraction around the implant were measured by CT specimens, the expression of TGF-β1 protein around the implant was measured by immunohistochemistry, the level of TGF-β1 mRNA around the implant was measured by RT-PCR. Results: Before treatment, the blood sugar level of the model group and the compound Danshen group were significantly(P<0.05) higher than the control group,, while the insulin level was significantly(P<0.05) reduced. After treatment, compared with the model group, the blood glucose and insulin of the compound Danshen group significantly(P<0.05) decreased, and the number of trabecular bone, thickness of trabecular bone, bone volume fraction, maximum extraction force of implant and level of TGF-β1 around implant in model group and compound danshen group were significantly (P<0.05) reduced, but the trabecular space was increased (P<0.05). Furthermore, the number of trabecular bone, thickness of trabecular bone, bone volume fraction, maximum extraction force of implant, and level of TGF-β1 around implant in compound danshen group were higher than those in the model group (P<0.05), while the trabecular space was lower than that of model group (P<0.05).Conclusion: Compound danshen increases the expression of TGF-β1 around the implant in diabetic rats and promote implant bone cementation.

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