HPV18E7和p16蛋白在老年患者口腔癌中的表达-东南大学学报医学版

HPV18E7和p16蛋白在老年患者口腔癌中的表达

单位：华中科技大学同济医学院附属协和医院口腔科, 湖北武汉 430000

分类号：R349.52

出版年·卷·期（页码）：2019·38·第四期（633-637）

摘要：

目的：探讨HPV18E7、p16蛋白在口腔癌患者口腔中的表达及其与口腔癌的临床分期、病理分级的关系。方法：采用免疫组织化学方法检测口腔癌组（173例）、口腔癌癌前病变组（98例）及癌周正常组织组（79例）HPV18E7、p16蛋白的表达，并分析口腔癌不同临床分期和病理分级口腔癌中HPV18E7、p16蛋白阳性表达情况。结果：口腔癌组HPV18E7阳性表达率为81.5%（141/173），p16阳性表达率为23.7%（41/173）；口腔癌癌前病变组分别为39.8%（39/98）和57.1%（56/98）；口腔癌癌周正常组织组分别为2.5%（2/79）和89.9%（71/79）。3组HPV18E7、p16蛋白阳性表达率的差异均有统计学意义（P<0.05），HPV18E7在口腔癌中的阳性表达率最高，p16在口腔癌中的阳性表达率最低。随着口腔癌患者临床分期和病理分级的增加，HPV18E7阳性表达率增高，p16阳性表达率降低，差异有统计学意义（P<0.05）。结论：与口腔癌癌前病变组织以及癌周正常组织比，口腔癌组织的HPV18E7蛋白阳性表达率增高，p16蛋白阳性表达率降低，口腔癌的不同临床分期和病理分级的HPV18E7、p16蛋白的表达也有差异，HPV18E7、p16蛋白可能参与了口腔癌的发生与发展过程。

Objective: To investigate the expression of HPV18E7 and p16 proteins in oral cavity of oral cancer patients and its relationship with clinical stage and pathological grade of oral cancer. Methods: Immunohistochemistry was used to detect the expression of HPV18E7 and p16 in oral tissues of 173 oral cancer patients, 98 oral cancer precancerous lesions and 79 normal peritumoral tissues, and analyze the expression of HPV18E7 and p16 proteins with the oral clinical stages and pathological graded oral cavity. Results: The positive expression rate of HPV18E7 and p16 in oral cancer group were 81.5% (141/173) and 23.7% (41/173), 39.8% (39/98) and 57.1% (56/98) in the oral cancer precancerous lesion group, 2.5% (2/79) and 89.9% (71/79) in the normal tissue group. The positive expression rate of HPV18E7 and p16 were different in the three groups. HPV18E7 had the highest positive expression in oral cancer, and p16 had the lowest positive expression in oral cancer, the difference was statistically significant (P<0.05). With the increase of clinical stage and pathological grade of oral cancer patients, the positive expression of HPV18E7 increased, and the positive expression of p16 decreased, the difference was statistically significant (P<0.05). Conclusion: Compared with normal precancerous lesions and normal tissues of oral cancer, the positive expression of HPV18E7 protein was higher and p16 protein is lower in oral carcinoma tissues than that in oral precancerous tissues and normal tissues. There are significant differences in the expression of HPV18E7 protein and p16 protein in different clinical stages and pathological grades of oral carcinoma. HPV18E7 protein and p16 protein may be involved in the carcinogenesis and development of oral carcinoma.

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