>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
糖基化终产物对胚胎植入孕鼠子宫内膜功能和胚胎着床能力的影响
作者:程海玲  田君  王宁  霍会蚕  曹芹雪 
单位:河南大学淮河医院 妇产科, 河南 开封 475000
关键词:肥胖 糖基化终产物 蛋白 胚胎 小鼠 
分类号:R575.1
出版年·卷·期(页码):2019·38·第四期(606-611)
摘要:

目的:探讨肥胖相关的晚期糖基化终产物对胚胎植入孕鼠内膜功能和胚胎着床能力的影响。方法:选取30只雌性BABL/c小鼠,将其随机分为3组,即模型5 W组、模型10 W组及正常组,每组10只。采用高脂饲料喂养模型组小鼠,构建肥胖模型;正常组喂养与模型组等量的普通饲料。在造模后对小鼠进行植入胚胎。20 d后眼球取血检测各项指标,应用RT-PCR以及Western blotting检测基因和蛋白表达量。结果:在造模10周后,正常组小鼠的体质量基本不变;模型10 W组在第3、5和7周,模型5 W组在第5和7周与正常组相比体质量变化差异均具有统计学意义(P<0.05)。血浆胆固醇、三酰甘油、血糖、胰岛素含量模型5、10 W组与正常组相比差异均有统计学意义(P<0.05)。正常组胚胎存活率整体无太大差异,而模型10 W组由原来的存活93粒最终降低为25粒,在妊娠第10、20天模型组的胚胎存活率与正常组相比差异均有统计学意义(P<0.05)。与正常组比较,模型5、10W组组织中的RAGE基因表达量呈增高趋势,HOXA10 基因表达量呈下降趋势;RAGE蛋白表达量呈增高趋势,HOXA10、P/CAF、FKBP52、PTEN蛋白表达量呈下降趋势。结论:肥胖相关的晚期糖基化终产物是通过影响RAGE受体的表达,进而影响小鼠胚胎着床重要蛋白的表达,最终影响胎盘植入孕鼠内膜功能和胚胎着床能力。

Objective: To investigate the effects of obesity-related advanced glycosylated end-products on the placenta implantation and embryo implantation ability in pregnant mice. Methods: The adult female BABL/c mice were randomly divided into three groups, 10 mice in each group. The obesity model was established in mice who were fed with high-fat feed. The normal group was fed the same amount of ordinary feed, and the mice were implanted with embryos after the models were made. After 20 days, the mice were anesthetized, sacrificed and collected the blood from eyes, and then a series of indexes were detected, The gene and protein expression were detected by the method of RT-PCR and Western blotting. Results: Compared with the normal group, the weight of mice in the normal group remained basically unchanged, except for the 10W model group (P<0.05) in the 3th,5th and 7th week. The plasma cholesterol, triglyceride, blood glucose, and insulin contents in the 5 W model and the 10 W group were statistically significant compared with the normal group (P<0.05). The result of survival rate of embryos indicated that the live embryos reduced from 93 to 25 in the 10 W group, the survival rate of live embryos in two model groups had statistical significance (P<0.05) compared with the normal group. The RT-PCR results showed that, compared with the normal group, the expression levels of RAGE in the 5 W and 10 W model groups was increasing, while the expression levels of HOXA10 genes showed a decreasing trend. The Western blotting results showed that the expression levels of RAGE protein in the 5 W and 10 W model groups were increasing, the expression levels of HOXA10, P/CAF, FKBP52 and PTEN protein were decreasing. Conclusion: Obesity related advanced glycosylation end product influences the expression of important protein by influencing the RAGE receptor expression, ultimately affects the endothelial function and the ability of embryo and placenta implantation in pregnant mice.

参考文献:

[1] MAHABADI A A,MASSARO J M,ROSITO G A,et al.Association of pericardial fat,intrathoracic fat,and visceral abdominal fat with cardiovascular disease burden:the Framingham heart study[J].Eur Heart J,2009,30(4):850-856.
[2] BELLVER J,DE LOS SANTOS M J,ALAMÁ P,et al.Day-3 embryo metabolomics in the spent culture media is altered in obese women undergoing in vitro fertilization[J].Fertil Steril,2015,103(6):1407-1415.
[3] BONFRATE L,WANG D Q,GARRUTI G,et al.Portincasa obesity and the risk and prognosis of gallstone disease and pancreatitis[J].Best Pract Res Clin Gastroenterol,2014,28(6):623-635.
[4] TREMELLEN K L,SYEDI N,TAN S,et al.Metabolic endotoxaemia-a potential novel link between ovarian inflammation and impaired progesterone production[J].Gynecol Endocrinol,2015,31(3):309-312.
[5] RIGALLEAU V,COUGNARD-GREGOIRE A,NOV S,et al.Association of advanced glycation end products and chronic kidney disease with macroangiopathy in type 2 diabetes[J].J Diabetes Complications,2015,29(5):270-274.
[6] FABJAN J M,PANNEAU B,DUFFARD N,et al.In vitro production of small ruminant embryos:late improvements and further research[J].Theriogenology,2014,81(7):1149-1162.
[7] PARAMIO M T,IZQUIERDO D.Current status of in vitro embryo production in sheep and goats[J].Reprod Domest Anim,2014,49(4):37-48.
[8] PELLEGRINO C A,MOROTTI F,UNTURA R M,et al.Use of sexed sorted semen for fixed-time artificial insemination or fixed-time embryo transfer of in vitro-produced embryos in cattle[J].Theriogenology,2016,86(24):888-893.
[9] HAJIAN M,HOSSEINI S M,OSTADHOSSEINI S,et al.Comparative stepwise pattern of reactive oxygen species production during in vitro development of fertilized and nuclear transferred goat embryos[J].Int J Fertil Steril,2017,11(3):93-98.
[10] CHÉRET C L,GERVAIS A,LELLI A,et al.Neurotoxic activation of microglia is promoted by a nox1 dependent NADPH oxidase[J].J Neurosci,2008,28(46):12039-12051.
[11] KOMATSU K,KOYA T,WANG J,et al.Analysis of the effect of leukemia inhibitory factor on follicular growth in cultured murine ovarian tissue[J].Biol Reprod,2015,93(2):18.
[12] 邓凯贤,柳晓春,郑玉华,等.子宫内膜异位症内膜组织HOXA10基因异常DNA甲基化及意义[J].现代妇产科进展,2011,20(2):868-872.
[13] 陈爱兰,白洁,陈晶晶.子宫内膜异位症相关不孕症患者子宫内膜容受性及中药治疗的疗效评价[J].中国中医药信息杂志,2014,21(6):11-13.
[14] SOVERNIGO T C,ADONA P R,MONZANI P S,et al.Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production[J].Reprod Domest Anim,2017,52(4):561-569.
[15] PARAMIO M T,IZQUIERDO D.Recent advances in in vitro embryo production in small ruminants[J].Theriogenology,2016,86(9):152-159.
[16] ARSOV S,TRAJCESKA L,VAN OEVEREN W,et al.The influence of body mass index on the accumulation of advanced glycation end products in hemodialysis patients[J].Eur J Clin Nutr,2015,69(12):309-313.
[17] LORENZI R,PATTOU F,BEUSCART J B,et al.Anti-sRAGE autoimmunity in obesity:downturn after bariatric surgery is independent of previous diabetic status[J].Diabetes Metab,2014,40(24):356-362.
[18] RUSTANDI R R L,ANDERSON C,HAMM M.Application of capillary electrophoresis in glycoprotein analysis[J].Methods Mol Biol,2013,988(5):181-197.
[19] APPELTANT R,SOMFAI T,NAKAI M,et al.Interactions between oocytes and cumulus cells during in vitro maturation of porcine cumulus-oocyte complexes in a chemically defined medium:effect of enuded oocytes on cumulus expansion and oocyte maturation[J].Theriogenology,2015,83(6):567-576.
[20] CIOBANU D M,OLAR L E,STEFAN R,et al.Fluorophores advanced glycation end products (AGEs)-to-NADH ratio is predictor for diabetic chronic kidney and cardiovascular disease[J].J Diabetes Complications,2015,29(7):893-897.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 186620 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-87232481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364