>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
补气增液汤对慢传输型便秘大鼠肠道功能、肠神经递质及Cajal间质细胞的影响
作者:刘丽兵  邓秋林  汤国辉  邢佼涛  范超  阳瑶 
单位:南华大学附属南华医院 肛肠科, 湖南 衡阳 421002
关键词:补气增液汤 慢传输型便秘 神经递质 Cajal间质细胞 大鼠 
分类号:R-33;R256.35
出版年·卷·期(页码):2019·38·第三期(433-438)
摘要:

目的:探究补气增液汤对慢传输型便秘(STC)大鼠排便量、肠道功能、肠神经递质、Cajal间质细胞(ICC)及干细胞因子(SCF)/干细胞生长因子受体(c-kit)信号通路的影响。方法:选取50只雄性SD大鼠随机分为正常组、模型组、低剂量补气增液汤组、高剂量补气增液汤组和莫沙必利组,每组10只;采用复方地芬诺酯建立STC大鼠模型,检测造模前、造模期、治疗期大鼠粪便数量及含水率;检测各组大鼠肠神经递质一氧化氮(NO)、一氧化氮合酶(NOS)、P物质(SP)、血管活性肠肽(VIP)含量以及c-kit、SCF蛋白的表达。结果:与模型组相比,补气增液汤能够增加STC大鼠粪便数量及粪便含水率(P<0.05);与正常组比较,模型组大鼠结肠SP、VIP、NO及NOS含量升高(P<0.05);而与模型组比较,补气增液汤组神经递质VIP、NO和NOS水平下降(P<0.05),c-kit、SCF蛋白表达水平提高(P<0.05)。结论:补气增液汤能够改善便秘症状,其机制可能与减少结肠NO、NOS、VIP含量及调控SCF/c-kit信号通路,进而增强肠道平滑肌运动有关。

Objective:To explore the effects of Buqizengye decoction on defecation, intestinal function, intestinal neurotransmitters, Cajal interstitial cells(ICC) and SCF/c-kit signaling pathway in rats with slow transit constipation(STC). Methods:Fifty male SD rats were randomly divided into normal group, model group, low-dose group of Buqizengye decoction, high-dose group of Buqizengye decoction and Mosapride group with 10 rats in each group. The rat model of STC was established to detect the amount of feces and water content in rats before, during and after treatment.The intestinal neurotransmitters NO, NOS, SP and VIP, and the expression of c-kit and SCF protein were detected in each group. Results:Compared with the model group, Buqi zengye decoction could increase the fecal amount and water content of STC rats(P<0.05). Compared with the normal group, the levels of SP, VIP, NO and NOS in the colon of the model group increased(P<0.05). Compared with the model group, the levels of VIP, NO and NOS decreased in the Buqizengye decoction group(P<0.05), while the expression of c-kit and SCF protein was increased in the Buqizengye decoction group(P<0.05). Conclusion:Buqizengye decoction can improve the symptoms of constipation, and its mechanism may be related to the reduction of the content of NO, NOS, VIP in colon and the regulation SCF/c-kit signaling pathway, which can improve the smooth muscle movement of the intestine.

参考文献:

[1] 孟丽敏,李卫,刘伟,等.中老年功能性便秘患者症状与生活质量的相关性[J].中国老年医学杂志,2017,37(7):1754-1756.
[2] 师宏丽.西沙必利联合复方芦荟胶囊治疗老年功能性便秘[J].现代医学,2012,40(6):648-650.
[3] 陈萌,于永铎.化瘀通便汤对慢性传输型便秘大鼠结肠水通道蛋白1和水通道蛋白3的影响[J].辽宁中医药大学学报,2016,18(11):26-28.
[4] 李勇,王健,李毅平,等.芪榔合剂对慢性传输型便秘小鼠结肠SP、VIP的影响[J].世界华人消化杂志,2008,16(3):272-276.
[5] 谢振年,李东冰,贾小强,等.焦虑和抑郁在慢传输型便秘患者发病过程中的作用评估初探[J].世界中医药,2013,8(9):1033-1035.
[6] 贾蕊,邢海娇,张选平,等.不同刺灸法对功能性便秘大鼠血浆一氧化氮、一氧化氮合酶及结肠血管活性肠肽的影响[J].针刺研究,2017,42(1):50-55.
[7] 林仁敬,谭蕊.白术七物颗粒对气阴两虚型结肠慢传输型便秘大鼠结肠组织血管活性肠肽及P物质的影响[J].中国全科医学,2016,19(3):336-339.
[8] 孙建梅,顾立梅,李慧,等.运脾柔肝方对便秘型肠易激综合征大鼠5-HT、VIP及SP影响的实验研究[J].南京中医药大学学报,2017,33(5):524-528.
[9] 王凯悦,余芝,梁超,等.针刺调节结肠慢传输便秘ICC相关机制的研究进展[J].世界华人消化杂志,2015,23(5):748-753.
[10] 肖开春,童荣生.Cajal间质细胞与胃肠动力关系的研究进展[J].中国药房,2013,24(5):470-472.
[11] MITSUI R,KOMURO T.Direct and indirect innervation of smooth muscle cells of rat stomach with special reference to the interstitial cells of Cajal[J].Cell Tissue Res,2002,309(2):219-227
[12] 陈立军,张廷模,彭成.大黄不同炮制品对热结便秘模型大鼠结肠c-kit mRNA表达的影响[J].中药药理与临床,2009,25(4):37-40.
[13] CHAI Y,HUANG Y,TANG H,et al.Role of stem cell growth factor/c-Kit in the pathogenesis of irritable bowel syndrome[J].Exp Ther Med,2017,13(4):1187-1193.
[14] LIANG J,WU YL,CHEN BJ,et al.The C-kit receptor mediated signal transduction and tumor-related diseases[J].Int J Biol Sci,2013,9(5):435-443.
[15] 柴玉娜,唐洪梅,黄育生,等.腹泻型肠易激综合征大鼠SCF/C-kit信号的变化及其与免疫功能的关系[J].2018,34(1):68-72.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 175413 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-87232481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364