Objective:To explore the purine-rich element binding protein alpha(PURα)-mediated expression of p27kip-1and tumor necrosis factor alpha(TNF-α) in the pathogenesis of diabetic neuropathy(DN). Methods:DN rats were prepared by intraperitoneal injection of streptozotocin as the DN group, and healthy rats were used as the control group with 20 rats in each group. The pathological pain and thermal hyperalgesia were compared between the two groups within one week. The expression levels of PURα, p27kip-1 and TNF-α in dorsal root ganglion(DRG) neurons in the two groups were analyzed by Western blot. The mouse hippocampal neuron cell line HT-22 was transfected into 4 groups,i.e., the PURα overexpression group (transfected with PURα expression plasmid), the mock group(without transfection of any expression vector),the blank control group (transfected empty expression vector) and the PURα silencing group (transfected with PURα RNAi expression plasmid), and the expression of PURα, p27kip-1 and TNF-α in the four cell lines groups were determined. Results:Compared with the control group, the tactile allodynia and thermal hyperalgesia were increased,and the expression levels of PURα, p27kip-1 and TNF-α was up-regulated in DRG neurons of the DN group(P<0.05). PURα was significantly and positively correlated with the expression of p27kip-1 and TNF-α(P<0.05). Up-regulation of PURα expression in neuronal cell lines significantly increased the expression of p27kip-1 and TNF-α(P<0.05).As the expression of endogenous PURα was inhibited, the expression levels of p27kip-1 and TNF-α were significantly decreased(P<0.05). Conclusion:PURα can mediate the expression of p27kip-1 and TNF-α protein, which may be involved in the pathogenesis of DN.
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