槐耳清膏对乳腺癌MCF-7细胞裸鼠移植瘤的抑制作用及其与调控miR-126表达的关系-东南大学学报医学版

槐耳清膏对乳腺癌MCF-7细胞裸鼠移植瘤的抑制作用及其与调控miR-126表达的关系

单位：1. 三峡大学第一临床医学院肿瘤防治中心&宜昌市中心人民医院肿瘤科, 湖北宜昌 443000;
2. 武汉市中心医院武汉市肿瘤研究所, 湖北武汉 430061

分类号：R285.6;R-33;R737.9

出版年·卷·期（页码）：2019·38·第二期（328-332）

摘要：

目的：探讨槐耳清膏对乳腺癌MCF-7细胞裸鼠致瘤性生长抑制作用及其与调控miR-126表达的关系。方法：建立裸鼠乳腺癌MCF-7细胞皮下移植瘤模型，随机将裸鼠分为两组，实验组给予槐耳清膏（浓度150 mg·ml^-1）0.2 ml灌胃，对照组采用0.9%生理盐水0.2 ml灌胃，每天两次，共30 d，观察裸鼠瘤体积及瘤体重的变化；RT-PCR检测裸鼠移植瘤组织中miR-126的表达。结果：（1）裸鼠在接种MCF-7细胞3周后，实验组裸鼠移植瘤体重为（0.20±0.07）g，而对照组裸鼠移植瘤体重为（0.57±0.32）g，实验组裸鼠移植瘤体重明显低于对照组，差异具有统计学意义（P<0.01）。（2）实验组裸鼠移植瘤体积为（0.32±0.74）cm³，而对照组裸鼠移植瘤体积为（0.84±0.62）cm³，实验组移植瘤体积较对照组小，差异具有统计学意义（P<0.01）。（3）裸鼠移植瘤组织中miR-126表达实验组是对照组的2.406倍，差异具有统计学意义（P<0.05）。结论：槐耳清膏能抑制裸鼠乳腺癌MCF-7细胞的生长，其机制可能是通过上调miR-126的表达从而抑制乳腺癌的发生和发展。

Objective:To explore the inhibitive effect of huaier cream on xenografted human MCF-7 cells in nude mice and its role in the regulation of miR-126 expression. Methods:Tumor models were established by subcutaneously transplanting human MCF-7 cells into nude mice and then which were randomly divided into two groups. The experimental group received 0.2 ml Huaier cream (150 mg·ml^-1) lavage twice per day for a period of 30 days, while 0.2 ml of 0.9% saline was used in the control group. After sacrificing the mice, tumor volume and weight were accordingly measured and recorded. RT-PCR was used to measure the expression of miR-126. Results:(1) All nude mice developed tumors at the transplanted site 3 weeks after inoculation with MCF-7 cells. The transplanted tumors were significantly lighter in the experimental group[(0.20±0.07)] g when compared with those in the control group[(0.57±0.32)] g (P<0.01). (2) Tumor volumes were significantly lower in the experimental group than those in the control group[(0.32±0.74) cm³ vs (0.84±0.62) cm³, P<0.01]. (3) The expression of miR-126 in the experimental group was 2.406 times as high as in the control group, the difference was statistically significant (P<0.05). Conclusion:Huaier cream can significantly inhibit the growth of MCF-7 cells in nude mice,which might be through the upregulation of miR-126 expression, thereby inhibiting the occurrence and development of breast cancer.

参考文献：

[1] 邓运宗,于冬冬,郭寒冰.中医药院校肿瘤学科建设探索——"肿瘤舒适医疗"理念的建立与构架[J].中医药管理杂志,2017,25(11):8-11.
[2] FAN L,STRASSER-WEIPPL K,LI J J,et al.Breast cancer in Chian[J].Lancet Oncol,2014,15(7):e279-e289.
[3] 程万宏,杨争秒.愧耳清膏对人肺腺癌2A细胞生物学行为影响及相关机制研究[J].辽宁中医药大学学报,2016,18(3):29-31.
[4] 鲁明骞,卢宏达,许新华,等.槐耳清膏对人肺腺癌A549细胞增殖及凋亡的影响[J].中国老年学杂志,2017,37(11):2620-2621.
[5] 杨爱琳,胡仲冬,屠鹏飞.槐耳清膏抑制人前列腺癌PC3细胞增殖和侵袭作用及其机制研究[J].中国中药杂志,2016,41(14):2701-2705.
[6] 刘学军,杜娟.槐耳清膏对荷瘤小鼠瘤细胞Bcl-2、Bxa表达的影响[J].安徽中医学院学报,2010,29(2):60-61.
[7] 余安平,李雄英,李凌.槐耳颗粒联合沙利度胺抑制鼠肝癌H22细胞种植瘤的实验[J].肿瘤防治研究,2013,40(9):834-838.
[8] WANG X,QI W,LI Y,et al.Huaier extract induces autophagic cell death by inhibiting the mTOR/S6K pathway in breast cancer cells[J].PLoS One,2015,10(7):e131771.
[9] 鲁明骞,冯雪松,孔庆志,等.槐耳颗粒对乳腺癌术后化疗患者免疫功能及生活质量的影响[J].东南大学学报(医学版),2017,36(4):567-571.
[10] 毛思颖,曹海明,陈前军.槐耳颗粒治疗乳腺癌的研究进展[J].中成药,2016,38(6):1363-1367.
[11] ZOU Y,XIONG H,XIONG H,et al.A polysaccharide from mushroom Huaier retards human hepatocellular carcinoma growth,angiogenesis,and metastasis in nude mice[J].Tumour Biol,2015,36(4):2929-2936.
[12] 周大为.槐耳颗粒对乳腺癌动物模型Ki67、p53、CerbB-2蛋白表达的影响[J].新中医,2015,47(12):224-226.
[13] HAMMOND S M.An overview of micro RNAs[J].Adv Drug Deliv Rev,2015,87:3-14.
[14] PERGE P,NAGY Z,IGAZ I,et al.Suggested roles for micro RNA in tumors[J].Biomol Concepts,2015,6(2):149-155.
[15] SELLITTI D F,DOI S Q.Micro RNAs in renal cell carcinoma[J].Microrna,2015,4(1):26-35.
[16] IORIO M V,FERRACIN M,LIU G G,et al.MicroRNA gene expression deregllation in human breast cancer[J].Cancer Res,2005,65(16):7065-7070.
[17] EBRAHIMI F,GOPALAN V,SMITH R A,et al.miR-126 in human cancers:clinical roles and current perspectives[J].Exp Mol Pathol,2014,96(1):98-107.
[18] LU Y Y,SWEREDOSKI M J,HUSS D,et al.Prometastatic GPCR CD97 is a direct target of tumor suppressor microRNA-126[J].ACS Chem Biol,2014,9(2):334-338.
[19] VIMALRAJ S,MIRANDA P J,RAMYAKRISHNA B,et al.Regulation of breast cancer and bone metastasis by microRNAs[J].Dis Markers,2013,35(5):369-387.
[20] WANG C Z,YUAN P,LI Y.MiR-126 regulated breast cancer cell invasion by targeting ADAM9[J].Int J Clin Exp Pathol,2015,8(6):6547-6553.
[21] ZHANG Y,YANG P Y,SUN T,et al.miR-126 and miR-126*repress recruitment of mesenchymal stem cells and inflammatory monocytes to inhibit breast cancer metastasis[J].Nat Cell Biol,2013,15(3):284-294.
[22] TABAZOIE S F,ALARCON C,OSKARSSON T,et al.Endogenous human microRNAs tha suppress breast cancer carcinoma[J].Nature,2008,451(7175):147-152.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录