蒲公英甾醇对脂多糖诱导乳腺炎大鼠的抗炎作用及其机制-东南大学学报医学版

蒲公英甾醇对脂多糖诱导乳腺炎大鼠的抗炎作用及其机制

单位：河南中医药大学第一附属医院乳腺外科, 河南郑州 450000

分类号：R-33;R655.8

出版年·卷·期（页码）：2019·38·第二期（303-308）

摘要：

目的：探讨蒲公英甾醇对脂多糖（LPS）诱导乳腺炎大鼠的抗炎作用及其相关机制。方法：50只产后大鼠随机分为对照组、模型组、阳性对照组、蒲公英甾醇低剂量组和蒲公英甾醇高剂量组，乳导管内灌注LPS建立乳腺炎大鼠模型，分别以不同剂量蒲公英甾醇（5、10 mg·kg^-1）及地塞米松（5 mg·kg^-1）给药，24 h后取乳腺组织。进行组织切片观察乳腺组织的病理变化，采用ELISA法检测TNF-α、IL-1β和IL-6水平，采用RT-PCR检测乳腺组织iNOS、COX-2 mRNA表达水平，免疫印迹法检测乳腺组织NF-кB、MAPKS信号通道蛋白水平。结果：与模型组比较，蒲公英甾醇低剂量组和高剂量组大鼠乳腺组织TNF-α、IL-1β、IL-6、iNOS mRNA、COX-2 mRNA、磷酸化I-κB、NF-κB-p65、JNK、ERK、p38的表达水平显著下降（P<0.05）。结论：蒲公英甾醇可降低iNOS和COX-2 mRNA表达，抑制NF-κB、MAPKs信号通路和促炎细胞因子的产生，对乳腺炎发挥抗炎作用。

Objective:To investigate the anti-inflammatory effect and related mechanisms of taraxasterol on lipopolysaccharide (LPS)-induced mastitis rats. Methods:Fifty rats were randomly divided into normal group, model group, positive control group, taraxasterol low dose group and taraxasterol high group. Mastitis model rats were establish by LPS injected into mammary ducts. Mammary tissue was obtained after 24 h since administration of different doses of taraxasterol (5 mg·kg^-1, 10 mg·kg^-1) or dexamethasone(5 mg·kg^-1). The pathological changes of breast tissue were observed by tissue section. ELISA was used to determine the levels of TNF-α, IL-1β and IL-6. RT-PCR was applied to determine the expression of iNOS、COX-2 mRNA in mammary tissue. Western-blot was used to determine the protein levels of NF-кB, MAPKS signal pathways in mammary tissue. Results:Compared with model group, expression levels of TNF-α, IL-1β, IL-6, iNOS mRNA, COX-2 mRNA, phosphorylate I-κB, NF-κB-p65, JNK, ERK, p38 in taraxasterol low dose group and taraxasterol high group were significantly decreased(P<0.05). Conclusion:Taraxasterol plays anti-inflammatory effect by reducing the expression of iNOS and COX-2 mRNA, inhibiting NF-κB and MAPKs signal pathways, inhibiting the production of inflammatory cytokines.

参考文献：

[1] 吴青.哺乳期急性乳腺炎的强化护理与预防[J].中医临床研究,2017,9(19):137-138.
[2] 孙英红.蒲公英治疗急性乳腺炎[J].中国民间疗法,2016,24(11):72-73.
[3] 抗晶晶,王辉.蒲公英三萜类物质的抗炎作用研究进展[J].中国野生植物资源,2015,34(1):37-39.
[4] 陈玉时,朱金华,曾伟.细菌内毒素及其拮抗药物的研究概况[J].亚太传统医药,2011,7(9):162-165.
[5] 解颖颖,胡学权,崔京春,等.脂多糖诱导哺乳期SD大鼠乳腺炎模型的建立[J].动物医学进展,2017,38(2):46-50.
[6] 王晓晨,吉爱国.NF-κB信号通路与炎症反应[J].生理科学进展,2014,45(1):68-71.
[7] LI D,ZHANG N,CAO Y,et al.Emodin ameliorates lipopolysaccharide-induced mastitis in mice by inhibiting activation of NF-κB and MAPKs signal pathways[J].Eur J Pharmacol,2013,705(1-3):79-85.
[8] 陶颖娜,万华.哺乳期乳腺炎免疫发病机制的研究进展[J].医学综述,2017,23(12):2333-2338.
[9] 张轶,马祥君,高海凤.夏枯草口服液对慢性乳腺炎患者炎症水平、VAS评分和肿块大小的影响[J].现代医学,2018,46(8):911-915.
[10] 冯宁霞,丁文波,史发兰.52例浆细胞性乳腺炎患者超声图像特征分析[J].现代医学,2018,46(6):625-628.
[11] 赵慧朵,程旭锋,楚爱景.栀子苷对乳腺炎小鼠乳腺组织及乳腺上皮细胞中IL-6,TNF-α和IL-1β表达的影响[J].中国老年学,2017,37(19):4712-4714.
[12] 高君,沈洪兵.诱导型一氧化氮合酶与乳腺癌关系的研究进展[J].中国肿瘤,2007,16(12):991-993.
[13] 毛秋娴,龙启才.盐酸小檗碱对LPS诱导的小鼠RAW264.7细胞PGE2、COX-2表达的影响[J].现代医院,2008,8(12):7-9.
[14] 程瑶,郭泽,王平,等.蒲公英甾醇对LPS诱导的RAW264.7细胞iNOS和COX-2表达的影响及其机制研究[J].免疫学杂志,2015,31(10):860-863.
[15] 崔艳,曹小平.核转录因子-κB的研究进展[J].川北医学院学报,2014,29(5):510-513.
[16] 高黎,赵志英.MAPK信号转导通路及其意义[J].世界最新医学信息文摘,2015,15(53):25-27.

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录