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尼莫地平治疗大鼠颈动脉狭窄所致认知功能障碍的研究
作者:郑文婧1  郄晓娟2  郑文芳3  王忠义1  张同军1 
单位:1. 衡水市人民医院 麻醉科, 河北 衡水 053000;
2. 河北医科大学第三医院 麻醉科, 河北 石家庄 050000;
3. 衡水市妇幼保健院 麻醉科, 河北 衡水 053000
关键词:尼莫地平 颈动脉狭窄 术后认知功能障碍 大鼠 
分类号:R-33;R741;R543.4
出版年·卷·期(页码):2019·38·第二期(291-296)
摘要:

目的:探讨尼莫地平治疗大鼠颈动脉狭窄所致术后认知功能障碍(POCD)的效果。方法:选取54只SD大鼠采用随机数字表法分为假手术组、狭窄组及尼莫地平组(n=18)。尼莫地平组大鼠于造模成功2周后腹腔注射1 mg·kg-1尼莫地平,假手术组及狭窄组给予等量生理盐水,连续4周,每天1次。分别于治疗前、治疗后2周、治疗后4周检测大鼠空间学习记忆能力,海马神经递质含量,海马组织结构变化以及Bax,Bcl-2,裂解的含半胱氨酸的天冬氨酸蛋白水解酶3、9蛋白表达。结果:与狭窄组比较,尼莫地平组治疗后相应时间点狭窄率降低、逃避潜伏期缩短(P<0.01),平均平台象限游泳距离百分比增加(P<0.01);治疗4周后与狭窄组比较,尼莫地平组大鼠海马神经元形态较为一致,NE、DA及5-HT含量升高(P<0.01),Bax及含半胱氨酸的天冬氨酸蛋白水解酶3、9表达量下调(P<0.01),Bcl-2表达量上调(P<0.01)。结论:尼莫地平可通过抑制神经元凋亡改善颈动脉狭窄导致的大鼠POCD。

Objective:To explore effect of nimodipine on post-operative cognitive dysfunction (POCD) in rats with carotid artery stenosis. Methods:Fifty-four rats were randomly divided into sham operation group, stenosis group and nimodipine group with 18 rats in each group. After two weeks, the rats were injected with 1 mg·kg-1 nimodipine intraperitoneally in nimodipine group, and the rats in the sham operation group and the stenosis group were given equal amount of saline for 4 weeks. The spatial learning and memory ability, hippocampal neurotransmitter content, hippocampal tissue structure, expression of B lymphocyte tumor -2 (Bcl-2), Bcl-2 related X protein (Bax), cleaved aspartic acid protein hydrolase 3 (Caspase 3) and 9 protein were detected before treatment, 2 weeks and 4 weeks after treatment. Results:Compared with the stenosis group, the stenosis rate and escape latency were decreased(P<0.01), average platform quadrant swimming distance percentage was increased(P<0.01) in the nimodipine group before and after treatment at the same time. After 4 weeks treatment,compared with the stenosis group, morphology of hippocampal neurons was a more consistent. The contents of NE, DA and 5-HT were increased (P<0.01), and the expressions of Bax, cleaved caspase 3 and 9 were decreased (P<0.01), and the expression of Bcl-2 was increased in the nimodipine group (P<0.01). Conclusion:Nimodipine can improve POCD in rats with carotid artery stenosis via inhibition neurons apoptosis.

参考文献:

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