目的：探讨过氧化物酶体增殖剂激活受体（PPAR）γ激动剂罗格列酮对钙/钙调蛋白依赖性丝氨酸蛋白激酶（CASK）的影响，探索CASK是否参与罗格列酮对胰岛素分泌的调控作用。方法：用不同浓度罗格列酮处理胰岛β细胞系INS-1细胞检测氯化钾刺激胰岛素分泌（KSIS）；用不同浓度及时间罗格列酮处理INS-1细胞，检测CASK的mRNA、蛋白及定位变化；观察干扰CASK后罗格列酮对KSIS的影响情况。结果：罗格列酮处理后INS-1细胞的KSIS功能明显增强（P<0.05）；不同浓度及时间罗格列酮处理INS-1细胞后CASK的mRNA和蛋白水平呈剂量及时间依赖性增加（P<0.05）；干扰CASK后罗格列酮对KSIS的影响差异无统计学意义（P>0.05）。结论：PPARγ激动剂罗格列酮上调INS-1细胞CASK基因的表达，但CASK并不参与罗格列酮对胰岛分泌的调控作用。

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