Objective:To detect the expression of NLRP3, Caspase-1, IL-1β and IL-18 in the serum of preeclampsia patients and to explore its clinical relevance with preeclampsia. Methods:A total of 320 single pregnant women of Han nationality were included in the obstetrics department of Zhongda hospital affiliated to southeast university from January 2016 to January 2018. They were divided into two groups:preeclampsia group(160 cases)and control group(160 cases). The clinical data of the subjects were compared and the risk factors of PE were analyzed. 30 single pregnant women of Han nationality between July 2017 and December 2017 were selected, including 15 cases of early onset preeclampsia and 15 cases in control. The serum levels of NLRP3, Caspase-1, IL-1βand IL-18 were measured by ELISA. Results:1. There were statistically significant differences in age, partner age, education level, pre-pregnancy weight and body mass index(BMI), pre-childbirth weight and BMI and family history of hypertension between the two groups(P<0.05). 2. The serum levels of NLRP3, Caspase-1, IL-1 serum and IL-18 in pregnant women with early-onset PE were all increased compared with the control group, and the serum level of IL-1 was statistically significant(P<0.05). Conclusion:1. Low educational level, high pre-pregnancy BMI and family history of hypertension are the independent risk factors of PE. 2. There is an important relevance between pyroptosis and the pathogenesis of PE, which may be a new target for the treatment of PE. |
[1] 中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2015)[J].中华妇产科杂志,2015,50(10):721-728.
[2] 谢幸,苟文丽.妇产科学[M].8版,北京:人民卫生出版社,2014:64.
[3] 严欢,张殊,林其德.子痫前期相关miRNAs的研究进展[J].国际妇产科学杂志,2016(4):377-380.
[4] ADAM I,HAGGAZ A D,MIRGHANI O A,et al.Placenta previa and pre-eclampsia:analyses of 1645 cases at medani maternity hospital,Sudan[J].Front Physiol,2013,4:32.
[5] 鲜恩英,何春华,付振琳,等.西藏拉萨地区孕妇妊娠期高血压疾病相关危险因素研究[J].东南大学学报:医学版,2017,36(4):586-590.
[6] 田玲,马显显,朱立平,等.子痫前期初产妇妊娠结局比较及影响因素分析[J].宁夏医科大学学报,2017,39(11):1331-1334.
[7] MCDONALD S D,YUSUF S,WALSH M W,et al.Increased cardiovascular risk after pre-eclampsia in women with dysglycaemia[J].Diabet Med,2013,30(1):e1-e7.
[8] 侯磊,王欣,邹丽颖,等.肥胖对妊娠结局及分娩方式的影响[J].武警医学,2016,27(6):571-573.
[9] BOYD H A,TAHIR H,WOHLFAHRT J,et al.Associations of personal and family preeclampsia history with the risk of early-intermediate-and late-onset preeclampsia[J].Am J Epidemiol,2013,178(11):1611-1619.
[10] INGRID CW,MARIANA RV,MARIANA LM,et al.Increased expression of NLRP3 inflammasome in placentas from pregnant women with severepreeclampsia[J].J Reprod Immunol,2017,123:40-47.
[11] 段利利,刘子冬,王林,等.C1q和肿瘤坏死因子相关蛋白4(CTRP4)降低子痫前期大鼠滋养层细胞的Caspase-1和IL-1β水平[J].细胞与分子免疫学杂志,2016,32(11):1441-1445,1452.
[12] 崔天盆,郑承红,陈杰,等.细胞死亡方式及其清除[J].中国免疫学杂志,2014(6):843-846.
[13] KAYAGAKI N,STOWE I B,Lee B L,et al.Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling[J].Nature,2015,526(7575):666-671.
[14] BANDERA A,MASETTI M,FABBIANI M,et al.The NLRP3 inflammasome is upregulated in HIV-infected antiretroviral therapy-treated individuals with defective immune recovery[J].Front Immunol,2018,9(214):1-8.
[15] XU Y J,ZHENG L,HU Y W,et al.Pyroptosis and its relationship to atherosclerosis[J].Clin Chim Acta,2018,476:28-37.
[16] VANDANMAGSAR B,YOUM Y,RAVUSSIN A,et al.The NLRP3 inflammasome instigates obesity-induced inflammation and insulin resistance[J].Nat Med.2011,17(2):179-214.
[17] 罗蓓蓓.NLRP3炎症小体对糖尿病性心肌病的影响及其机制研究[D].济南:山东大学,2014.
[18] ChU Q,JIANG Y,ZHANG W,et al.Pyroptosis is involved in the pathogenesis of human hepatocellular carcinoma[J].Oncotarget,2016,7(51):84658-84665.
[19] 潘杨.NLRP3炎症小体在帕金森病神经炎症中的作用及治疗学意义研究[D].南京:南京医科大学,2016.
[20] MAGNA M,PISETSKY D S.The role of cell death in the pathogenesis of SLE:Is pyroptosis the missing link?[J].Scand J Immunol,2015,82(3):218-224. |