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早发型子痫前期患者血清细胞焦亡相关因子的表达及临床分析
作者:乔东艳1  孙白云2  虞天一1  史天一1  沈肖1  于红1 
单位:1. 东南大学附属中大医院 妇产科, 江苏 南京 210009;
2. 苏州大学附属第二医院 妇产科, 江苏 苏州 215004
关键词:子痫前期 早发型 NLRP3炎性小体 半胱氨酸天冬氨酸酶-1 白细胞介素18 白细胞介素1β 
分类号:R714.46
出版年·卷·期(页码):2019·38·第一期(145-149)
摘要:

目的:检测早发型子痫前期患者血清中NLRP3、Caspase-1、IL-1β、IL-18的表达并探索其临床意义。方法:选取2016年1月至2018年1月在东南大学附属中大医院产科住院分娩的汉族单胎孕妇320例,子痫前期及正常妊娠孕妇各160例。比较纳入对象的临床资料,采用多因素Logistic回归方法分析子痫前期发病的高危因素。选取其中2017年7月至2017年12月间住院分娩汉族单胎孕妇30例,早发型子痫前期及正常妊娠孕妇各15例。采用ELISA法检测两组孕妇血清中NLRP3、Caspase-1、IL-1β、IL-18的表达情况。结果:1.子痫前期组与正常妊娠组孕妇及伴侣年龄、文化程度、孕前及分娩前的体重、体重指数(BMI)、高血压家族史有统计学差异(P<0.05)。2.早发型子痫前期孕妇血清中NLRP3、Caspase-1、IL-1β、IL-18的表达水平较正常妊娠组均升高,其中IL-1β差异显著(P<0.05)。结论:(1)低文化程度、孕前高体重指数及高血压家族史为子痫前期独立危险因素。(2)细胞焦亡与子痫前期的发病机制有重要联系,或可成为治疗子痫前期的新靶点。

Objective:To detect the expression of NLRP3, Caspase-1, IL-1β and IL-18 in the serum of preeclampsia patients and to explore its clinical relevance with preeclampsia. Methods:A total of 320 single pregnant women of Han nationality were included in the obstetrics department of Zhongda hospital affiliated to southeast university from January 2016 to January 2018. They were divided into two groups:preeclampsia group(160 cases)and control group(160 cases). The clinical data of the subjects were compared and the risk factors of PE were analyzed. 30 single pregnant women of Han nationality between July 2017 and December 2017 were selected, including 15 cases of early onset preeclampsia and 15 cases in control. The serum levels of NLRP3, Caspase-1, IL-1βand IL-18 were measured by ELISA. Results:1. There were statistically significant differences in age, partner age, education level, pre-pregnancy weight and body mass index(BMI), pre-childbirth weight and BMI and family history of hypertension between the two groups(P<0.05). 2. The serum levels of NLRP3, Caspase-1, IL-1 serum and IL-18 in pregnant women with early-onset PE were all increased compared with the control group, and the serum level of IL-1 was statistically significant(P<0.05). Conclusion:1. Low educational level, high pre-pregnancy BMI and family history of hypertension are the independent risk factors of PE. 2. There is an important relevance between pyroptosis and the pathogenesis of PE, which may be a new target for the treatment of PE.

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