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烧伤患者肢体Ⅲ度创面组织恢复过程中EPO、TGFβ1及受体表达
作者:王乡宁  黄雷  邓利  杜佳 
单位:四川省成都市郫都区人民医院 第五外科, 四川 成都 611730
关键词:烧伤 肢体 Ⅲ度创面 促红细胞生成激素 转化生长因子-β1 受体 
分类号:R644
出版年·卷·期(页码):2019·38·第一期(118-125)
摘要:

目的:考察烧伤患者肢体Ⅲ度创面组织恢复过程中促红细胞生成激素(EPO)、转化生长因子-β1(TGF-β1)及受体表达。方法:选择2014年5月至2017年12月在我院收治的106例单个肢体Ⅲ度烧伤患者为研究对象。愈合时间为2.0~9.5周,中位数6.0周,以所有患者的愈合时间的中位数为界,分为2组,愈合快组和愈合慢组,各53例。结果:两组随着时间的延长均有不同程度地愈合(P<0.05),与愈合慢组比,愈合快组每周的愈合面积均较高(P<0.05)。除了愈合时间,两组患者的性别、年龄、烧伤原因、手术距离烧伤后的时间、患肢、高血压、高血脂、其他心脑血管疾病及0周时烧伤组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ表达差异均无统计学差异(P>0.05),与愈合慢组比,愈合快组的年龄、糖尿病例数、烧伤面积和手术面积较少,4周时烧伤组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ较高(P<0.05),以愈合快慢为因变量,以年龄、糖尿病例数、烧伤面积、手术面积、4周时烧伤组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ为自变量,进行Logistic回归分析,年龄、4周时烧伤组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ可影响肢体Ⅲ度创面组织愈合,年龄越小,4周时烧伤组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ表达越高,肢体Ⅲ度创面组织愈合越快(P<0.05)。两组创面组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ 0周的表达相当(P>0.05),到2周时,EPO、EPO受体、TGF-β1、TβRI及TβRⅡ均增高(P<0.05),到4周时,EPO、EPO受体、TGF-β1、TβRI及TβRⅡ均降低(P<0.05),与愈合慢组比,愈合快组2周和4周的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ均明显增高(P<0.05)。结论:肢体Ⅲ度创面组织的EPO、EPO受体、TGF-β1、TβRI及TβRⅡ表达与愈合明显相关,EPO、EPO受体、TGF-β1、TβRI及TβRⅡ表达较高,创伤愈合较快。

Objective:To investigate the expression of erythropoietin (EPO), transforming growth factor-β1 (TGF-β1) and their receptors during the restoration of wounds in third-degree burn patients. Methods:From May 2014 to December 2017 in our hospital, 106 cases of single limb third-degree burn patients were selected as the research objects. According to the median of all patients' healing time (2 weeks~9.5 weeks, the median was 6.0weeks), the patients were divided into fast healing group and slow healing group, 53 cases in each group. Results:Both groups healed to different extents (P<0.05). Compared with the slow healing group, the healed area of the healing group was bigger in the fast healing group (P<0.05). Except healing time, the sex, age, healing reasons, the healing limb, hypertension, hyperlipidemia and other cardiovascular diseases, the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue of 0 week in both groups had no differences (P>0.05). Compared with the slow healing group, the age, hyperlipidemia, the area of wound and surgery area were smaller (P<0.05), the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue of 4 weeks were higher in the fast healing group (P<0.05). The slow or the fast healing were as the dependent variables, the age, hyperlipidemia, the area of wound and surgery area, the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue of 4 weeks as the independent variables for logistical analyze. The age, the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue of 4 weeks could affect wound healing (P<0.05). The younger patients, the faster wound healing, and the higher expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue, the faster wound healing (P<0.05). At 0 week, the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ in wound tissue of 2 groups were comparable (P>0.05). At 2nd week, EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ had increased in both groups (P<0.05). At 4th weeks, EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ had decreased in both groups (P<0.05). Compared with the slow healing group, the expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ had increased in the fast healing group (P<0.05). Conclusion:The expression of EPO, EPO receptor, TGF-β1, TβRI and TβRⅡin wounds of the third-degree burns limbs were significantly correlated with the healing time, the higher EPO, EPO receptor, TGF-β1, TβRI and TβRⅡ are the faster the wound healing.

参考文献:

[1] BRASSOLATTI P,KIDO H W, BOSSINI P S, et al.Bacterial cellulose membrane used as biological dressings on third-degree burns in rats[J].Biomed Mater Eng,2018,29(1):29-42.
[2] MOHTASHAM A Z, TANIDEH N, SEDDIGHI A, et al.The Effect of lithospermum officinale, silver sulfadiazine and alpha ointments in healing of burn wound injuries in rat[J].World J Plast Surg,2017,6(3):313-318.
[3] 罗燕,徐崇明,段丽群.外源性信号素3A信号通路对转化生长因子-β1诱导肺癌细胞侵袭、增殖的影响[J].东南大学学报:医学版,2017,36(4):609-614.
[4] LOSKOTOVA A, LOSKOTOVA J.The use of acupuncture in first aid of burns-Clinical report[J].Burns,2017,43(8):1782-1791.
[5] GOKADZE S, BARBAKADZE V, MULKIJANYAN K, et al.Formulation and technology development of herbal phenolic biopolymer-containing films for burn treatment[J].Georgian Med News,2017,267(6):119-124.
[6] GERMANN G.Hand reconstruction after burn injury:Functional results[J].Clin Plast Surg, 2017, 44(4):833-844.
[7] GIRI P, EBERT S, BRAUMANN U D, et al.Skin regeneration in deep second-degree scald injuries either by infusion pumping or topical application of recombinant human erythropoietin gel[J].Drug Des Devel Ther,2015,9(4):2565-2579.
[8] TAN J, WU J.Current progress in understanding the molecular pathogenesis of burn scar contracture[J].Burns Trauma,2017,5(4):14.
[9] BURNS C M, KASZNIAK A W, CHEN K, et al. Longitudinal changes in serum glucose levels are associated with metabolic changes in Alzheimer's disease related brain regions[J].J Alzheimers Dis,2018,62(2):833-840.
[10] 蒋梅君,李泽,谢卫国.2133例电烧伤住院患者流行病学调查[J].中华烧伤杂志2017, 33(12):732-737.
[11] HAMED S, ULLMANN Y, EGOZI D, et al.Topical erythropoietin treatment accelerates the healing of cutaneous burn wounds in diabetic pigs through an aquaporin-3-dependent mechanism[J]. Diabetes,2017,66(8):2254-2265.
[12] ROCHA J, EDUARDO-FIGUEIRA M, BARATEIRO A, et al.Erythropoietin reduces acute lung injury and multiple organ failure/dysfunction associated to a scald-burn inflammatory injury in the rat[J].Inflammation,2015,38(1):312-326.
[13] HAMED S, BENNETT C L, DEMIOT C, et al.Erythropoietin, a novel repurposed drug:an innovative treatment for wound healing in patients with diabetes mellitus[J].Wound Repair Regen,2014,22(1):23-33.
[14] LIVNY E, LIVNAT T, YAKIMOV M, et al.Effect of erythropoietin on healing of corneal epithelial defects in rabbits[J].Ophthalmic Res,2013,50(2):129-133.
[15] GVNTER C I, BADER A, DORNSEIFER U, et al.A multi-center study on the regenerative effects of erythropoietin in burn and scalding injuries:study protocol for a randomized controlled trial[J].Trials,2013,14(4):124.
[16] KAMOLZ L P, SMOLLE-JUETTNER F, PARVIZI D.The use of erythropoietin in burns:sometimes good, sometimes not?[J]Crit Care Med,2013,41(4):1138-1139.
[17] BOHR S, PATEL S J, SHEN K, et al.Alternative erythropoietin-mediated signaling prevents secondary microvascular thrombosis and inflammation within cutaneous burns[J].Proc Natl Acad Sci U S A,2013,110(9):3513-3518.
[18] HORMOZI M, ASSAEI R, BOROUJENI M B.The effect of aloe vera on the expression of wound healing factors (TGFβ1 and bFGF) in mouse embryonic fibroblast cell:In vitro study[J].Biomed Pharmacother,2017,88(4):610-616.
[19] LI H, YAO Z, HE W, et al.P311 induces the transdifferentiation of epidermal stem cells to myofibroblast-like cells by stimulating transforming growth factor β1 expression[J].Stem Cell Res Ther,2016,7(1):175.
[20] 聂婵,王涛,施尚鹏,等.烧伤儿童住院时间及与烧伤面积关系的系统评价[J].东南大学学报:医学版,2017,36(2):246-253.
[21] BAI G, YAN G, WANG G, et al.Anti-hepatic fibrosis effects of a novel turtle shell decoction by inhibiting hepatic stellate cell proliferation and blocking TGF-β1/Smad signaling pathway in rats[J].Oncol Rep,2016,36(5):2902-2910.
[22] HAHN J M, MCFARLAND K L, COMBS K A, et al.Partial epithelial-mesenchymal transition in keloid scars:regulation of keloid keratinocyte gene expression by transforming growth factor-β1[J].Burns Trauma,2016,4(1):30.
[23] 李明,方勇,姚敏,等.转化生长因子β1受体抑制剂SD-208对人增生性瘢痕的作用[J].中华烧伤杂志,2016,32(7):389-395.
[24] TUUMINEN R, LOUKOVAARA S.Statin medication in patients with epiretinal membrane is associated with low intravitreal EPO, TGF-beta-1, and VEGF levels[J].Clin Ophthalmol, 2016,10(4):921-928.
[25] LUAN Y, ZHANG L, CHAO S, et al.Mesenchymal stem cells in combination with erythropoietin repair hyperoxia-induced alveoli dysplasia injury in neonatal mice via inhibition of TGF-β1 signaling[J].Oncotarget,2016,7(30):47082-47094.

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