Objective:A modified multiplex real-time fluorescence quantitative PCR method was established to detect the methylation of tumor suppressor genes MGMR, p16, CDH13 and RASSF1A, and to explore its application in early diagnosis of lung cancer. Methods:Thirty patients with lung cancer were confirmed by pathology and selected as lung cancer group and 30 patients with benign disease confirmed by pathology as control group. Establish a modified real-time fluorescence quantitative PCR system. Methylation of MGMT, p16, CDH13, and RASSF1A genes was detected in the subjects, and the positive rate of methylation was compared between the two groups. The sensitivity and specificity of the gene methylation alone and in combination for the early diagnosis of lung cancer were calculated. Results:Serum MGMT, p16, CDH13, and RASSF1A levels were significantly higher in lung cancer group than in the control group (P<0.05). The sensitivity and specificity of MGMT, p16, CDH13, and RASSF1A genes for the early diagnosis of lung cancer were significantly higher than those diagnosed separately (P<0.05). Conclusion:The established multiplex real-time fluorescence quantitative PCR method has been used to detect the methylation of MGMT, p16, CDH13 and RASSF1A genes. Which has high sensitivity and specificity for the early diagnosis of lung cancer.
 FITZMAURICE C,DICKER D,PAIN A.The Global burden of cancer 2013[J].JAMA Oncol,2015,1(4):505-527.
 GBD 2013 MORTALITY AND CAUSES OF DEATH COLLABORATORS.Global,regional,and national age-sex specific all-cause and cause-specific mortality for 240 causes of death,1990-2013:A systematic analysis for the global burden of disease study 2013[J].Lancet,2015,385(9963):117-171.
 YUAN S,YU Z,LIU Q,et al.GPC5,a novel epigenetically silenced tumor suppressor,inhibits tumor growth by suppressing Wnt/β-catenin signaling in lung adenocarcinoma[J].Oncogene,2016,35(47):6120-6131.
 CHUNG J H,LEE H J,KIM B H,et al.DNA methylation profile during multistage progression of pulmonary adenocarcinomas[J].Virchows Arch,2011,459(2):201-211.
 ZHANG Y,WANG R,SONG H,et al.Methylation of multiple genes as a candidate biomarker in non-small cell lung cancer[J].Cancer Lett,2011,303(1):21-28.
 RAJABI H,TAGDE A,ALAM M,et al.DNA methylation by DNMT1 and DNMT3b methyltransferases is driven by the MUC1-Concoprotein in human carcinoma cells[J].Oncogene,2016,35(50):6439-6445.
 BAYLIN S B, JONES P A.Epigenetic determinants of cancer[J].Cold Spring Harb Perspect Biol,2016,8(9):18.
 邸红芹, 杨永辉, 朱桂云,等. 改良多重实时荧光定量PCR检测流感A型和B型病毒[J]. 河北医药, 2016, 38(23):3529-3532.