α-硫辛酸抗兔动脉粥样硬化的作用及其机制探讨-东南大学学报医学版

α-硫辛酸抗兔动脉粥样硬化的作用及其机制探讨

单位：武汉市第一医院老年病科, 湖北武汉 430022

分类号：R363

出版年·卷·期（页码）：2019·38·第一期（28-33）

摘要：

目的：探讨α-硫辛酸（ALA）对兔动脉粥样硬化（AS）的作用及可能作用机制。方法：采用液氮冻伤术联合高脂饲养诱导兔发生AS病变，依据不同处理分为正常组、模型组和模型+ALA组。观测动脉组织病理情况和血脂变化，检测动脉组织中黏附分子（ICAM-1、VACM-1和P-选择素）和NF-κB的表达。结果：与正常组比较，模型组动脉内膜增厚，可见脂质斑块，兔血脂水平升高，动脉组织中黏附分子（ICAM-1、VACM-1和P-选择素）和NF-κB的表达显著增加（P<0.05）；与模型组比较，模型+ALA组脂质斑块面积减少，血脂降低，动脉组织中黏附分子和NF-κB表达降低（P<0.05）。结论：ALA可能通过影响NF-κB的表达抑制黏附分子的表达，抑制血管炎症反应，这可能是ALA改善AS病理进程的作用机制之一。

Objective:To investigate the effects and mechanisms of α-lipoic acid (ALA) in atherosclerosis (AS) rabbits. Methods:AS lesions were induced in rabbits receiving liquid nitrogen treatment and high fat diet. According to different treatment, rabbits were divided into normal group, model group and model+ALA group. Pathological conditions of arterial tissues and the changes of lipids were detected. The expression of cell adhesion molecules (ICAM-1, VACM-1, P-selection) and NF-κB protein were measured in arterial tissues. Results:Compared with the normal group, the thickness of arterial intima and the levels of serum lipids increased and plaques were observed in the model group, meanwhile, the expression of cell adhesion molecules (ICAM-1, VACM-1, P-selection) and NF-κB in arterial tissues significantly increased in the model group. Compared with the model group, the area of plaques and the levels of serum lipids reduced in the model+ALA group; and simultaneously the expression of cell adhesion molecules and NF-κB also decreased in the model+ALA group. Conclusion:α-lipoic acid may inhibit the expression of cell adhesion molecules by suppressing the expression of NF-κB, leading to the inhibition of vascular inflammation, which may be one of the mechanisms of ALA on improving pathology of AS.

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